Experiment: PUT8 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0788 and PP_0789 are separated by 88 nucleotides PP_0789 and PP_0790 overlap by 4 nucleotides PP_0790 and PP_0791 are separated by 44 nucleotides
PP_0788: PP_0788 - conserved protein of unknown function, at 903,742 to 905,901
_0788
PP_0789: PP_0789 - N-acetyl-anhydromuramyl-L-alanine-amidase, at 905,990 to 906,562
_0789
PP_0790: PP_0790 - Inner membrane protein AmpE, at 906,559 to 907,389
_0790
PP_0791: PP_0791 - putative hydrolase, at 907,434 to 908,210
_0791
Position (kb)
905
906
907 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 904.998 kb on - strand, within PP_0788 at 904.998 kb on - strand, within PP_0788 at 905.004 kb on + strand, within PP_0788 at 905.104 kb on - strand, within PP_0788 at 905.261 kb on + strand, within PP_0788 at 905.261 kb on + strand, within PP_0788 at 905.261 kb on + strand, within PP_0788 at 905.429 kb on + strand, within PP_0788 at 905.430 kb on - strand, within PP_0788 at 905.430 kb on - strand, within PP_0788 at 905.430 kb on - strand, within PP_0788 at 905.430 kb on - strand, within PP_0788 at 905.502 kb on + strand, within PP_0788 at 905.503 kb on - strand, within PP_0788 at 905.553 kb on - strand, within PP_0788 at 905.868 kb on - strand at 905.900 kb on - strand at 905.900 kb on - strand at 905.912 kb on + strand at 905.912 kb on + strand at 905.912 kb on + strand at 905.913 kb on - strand at 905.913 kb on - strand at 905.913 kb on - strand at 905.913 kb on - strand at 905.951 kb on + strand at 906.002 kb on + strand at 906.002 kb on + strand at 906.002 kb on + strand at 906.003 kb on - strand at 906.188 kb on + strand, within PP_0789 at 906.188 kb on + strand, within PP_0789 at 906.325 kb on + strand, within PP_0789 at 906.326 kb on - strand, within PP_0789 at 906.374 kb on + strand, within PP_0789 at 906.376 kb on + strand, within PP_0789 at 906.376 kb on + strand, within PP_0789 at 906.377 kb on - strand, within PP_0789 at 906.377 kb on - strand, within PP_0789 at 906.643 kb on - strand, within PP_0790 at 906.643 kb on - strand, within PP_0790 at 906.766 kb on + strand, within PP_0790 at 906.766 kb on + strand, within PP_0790 at 906.767 kb on - strand, within PP_0790 at 906.767 kb on - strand, within PP_0790 at 906.809 kb on - strand, within PP_0790 at 906.960 kb on + strand, within PP_0790 at 906.961 kb on - strand, within PP_0790 at 906.961 kb on - strand, within PP_0790 at 907.063 kb on - strand, within PP_0790 at 907.063 kb on - strand, within PP_0790 at 907.554 kb on + strand, within PP_0791
Per-strain Table
Position Strand Gene LocusTag Fraction PUT8 MOI 0.1 remove 904,998 - PP_0788 0.58 +1.1 904,998 - PP_0788 0.58 -0.4 905,004 + PP_0788 0.58 -0.1 905,104 - PP_0788 0.63 +0.6 905,261 + PP_0788 0.70 -1.0 905,261 + PP_0788 0.70 +0.1 905,261 + PP_0788 0.70 -1.5 905,429 + PP_0788 0.78 -0.8 905,430 - PP_0788 0.78 -0.1 905,430 - PP_0788 0.78 +0.8 905,430 - PP_0788 0.78 -1.5 905,430 - PP_0788 0.78 -0.1 905,502 + PP_0788 0.81 -2.4 905,503 - PP_0788 0.82 +0.7 905,553 - PP_0788 0.84 -0.2 905,868 - +0.4 905,900 - +0.4 905,900 - -0.2 905,912 + +1.0 905,912 + -0.2 905,912 + -0.0 905,913 - +0.4 905,913 - -1.9 905,913 - -1.5 905,913 - -0.9 905,951 + +1.8 906,002 + -4.2 906,002 + -5.0 906,002 + -4.8 906,003 - -4.6 906,188 + PP_0789 0.35 -4.3 906,188 + PP_0789 0.35 -3.7 906,325 + PP_0789 0.58 -1.8 906,326 - PP_0789 0.59 -5.7 906,374 + PP_0789 0.67 -1.5 906,376 + PP_0789 0.67 -5.5 906,376 + PP_0789 0.67 -4.9 906,377 - PP_0789 0.68 -1.2 906,377 - PP_0789 0.68 -5.6 906,643 - PP_0790 0.10 -0.2 906,643 - PP_0790 0.10 +1.1 906,766 + PP_0790 0.25 +0.3 906,766 + PP_0790 0.25 -0.2 906,767 - PP_0790 0.25 +1.0 906,767 - PP_0790 0.25 +0.4 906,809 - PP_0790 0.30 +0.7 906,960 + PP_0790 0.48 -0.7 906,961 - PP_0790 0.48 +0.2 906,961 - PP_0790 0.48 -0.1 907,063 - PP_0790 0.61 +0.0 907,063 - PP_0790 0.61 +0.7 907,554 + PP_0791 0.15 -0.1
Or see this region's nucleotide sequence