Strain Fitness in Pseudomonas putida KT2440 around PP_t32

Experiment: PUT 8 MOI 1

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	PUT 8 MOI 1
2,270,805	+		PP_2002	0.52	-1.3
2,270,806	-		PP_2002	0.52	+2.6
2,270,847	-		PP_2002	0.57	-3.7
2,270,884	-		PP_2002	0.62	+2.2
2,270,884	-		PP_2002	0.62	-5.6
2,271,171	+				-4.2
2,271,171	+				-0.3
2,271,171	+				+0.6
2,271,171	+				-0.2
2,271,171	+				+0.5
2,271,171	+				-3.9
2,271,171	+				+0.9
2,271,171	+				+0.7
2,271,172	-				-0.4
2,271,172	-				+0.2
2,271,172	-				-2.9
2,271,172	-				+1.4
2,271,172	-				+0.5
2,271,172	-				-2.1
2,271,172	-				-3.6
2,271,172	-				-4.0
2,271,173	+				-0.4
2,271,173	+				-1.4
2,271,174	-				+1.7
2,271,227	+				+1.2
2,271,228	-				+0.6
2,271,236	+				-1.4
2,271,249	+				-3.4
2,271,249	+				+1.4
2,271,250	-				+1.4
2,271,724	-		PP_t33	0.10	+0.2
2,271,811	+				-1.4
2,271,812	-				-1.6
2,271,812	-				-2.1
2,271,896	-				-1.4
2,271,896	-				-3.7
2,271,942	-		PP_t34	0.10	-3.1
2,272,179	+				+0.6
2,272,180	-				+1.3
2,272,180	-				-1.1
2,272,180	-				+0.9
2,272,328	-		PP_2003	0.25	+0.4
2,272,403	+		PP_2003	0.33	+0.3
2,272,403	+		PP_2003	0.33	-2.9
2,272,404	-		PP_2003	0.33	-3.4