Strain Fitness in Pseudomonas putida KT2440 around PP_5040

Experiment: PUT 8 MOI 1

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	PUT 8 MOI 1
5,741,233	+		PP_5038	0.62	+2.6
5,741,253	+		PP_5038	0.70	+0.3
5,741,253	+		PP_5038	0.70	-3.4
5,741,353	+				+0.6
5,741,353	+				-0.1
5,741,354	-				-0.4
5,741,354	-				+0.5
5,741,419	+				+0.7
5,741,420	-				+2.4
5,741,420	-				-0.7
5,741,420	-				-1.4
5,741,420	-				+1.1
5,741,427	+				+0.5
5,741,427	+				-4.3
5,741,490	+				+0.7
5,741,834	+		PP_5039	0.46	-3.4
5,741,915	+		PP_5039	0.60	+0.3
5,741,915	+		PP_5039	0.60	-4.1
5,742,050	+		PP_5039	0.83	-3.6
5,742,159	-				-4.2
5,742,159	-				-0.7
5,742,159	-				-4.2
5,742,159	-				-4.4
5,742,159	-				-4.6
5,743,272	+				-4.1
5,743,272	+				-2.3
5,743,273	-				-3.6
5,743,280	+				-3.6
5,743,348	+				-0.1
5,743,348	+				+0.0
5,743,349	-				-0.6
5,743,349	-				-2.5
5,743,445	+				+0.8
5,743,445	+				-3.1
5,743,565	+				-0.0
5,743,565	+				-0.0
5,743,565	+				+2.0
5,743,565	+				-3.3
5,743,566	-				+1.1
5,743,601	+				-3.7
5,743,632	-				+0.4
5,743,910	-		PP_5041	0.20	+0.9
5,743,964	+		PP_5041	0.22	-0.3
5,743,965	-		PP_5041	0.22	+0.3
5,744,070	-		PP_5041	0.27	+1.4
5,744,137	+				-0.9
5,744,137	+		PP_5041	0.29	-3.8
5,744,138	-		PP_5041	0.30	+0.2
5,744,147	+		PP_5041	0.30	-0.5
5,744,147	+		PP_5041	0.30	-4.3
5,744,148	-		PP_5041	0.30	+1.0
5,744,148	-		PP_5041	0.30	-3.9