Experiment: PUT 8 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_4868 and PP_4869 are separated by 3 nucleotides PP_4869 and PP_4870 are separated by 102 nucleotides PP_4870 and PP_4871 are separated by 347 nucleotides
PP_4868: PP_4868 - Nicotinate phosphoribosyltransferase, at 5,534,984 to 5,536,192
_4868
PP_4869: PP_4869 - NH(3)-dependent NAD(+) synthetase, at 5,536,196 to 5,537,023
_4869
PP_4870: PP_4870 - Azurin, at 5,537,126 to 5,537,575
_4870
PP_4871: PP_4871 - putative lysine decarboxylase family protein, at 5,537,923 to 5,538,510
_4871
Position (kb)
5537
5538 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 5536.154 kb on + strand at 5536.167 kb on + strand at 5536.167 kb on + strand at 5537.024 kb on + strand at 5537.024 kb on + strand at 5537.025 kb on - strand at 5537.025 kb on - strand at 5537.025 kb on - strand at 5537.025 kb on - strand at 5537.025 kb on - strand at 5537.057 kb on + strand at 5537.127 kb on + strand at 5537.127 kb on + strand at 5537.191 kb on - strand, within PP_4870 at 5537.191 kb on - strand, within PP_4870 at 5537.301 kb on + strand, within PP_4870 at 5537.302 kb on - strand, within PP_4870 at 5537.472 kb on + strand, within PP_4870 at 5537.473 kb on - strand, within PP_4870 at 5537.558 kb on + strand at 5537.599 kb on + strand at 5537.599 kb on + strand at 5537.642 kb on + strand at 5537.643 kb on - strand at 5537.697 kb on + strand at 5537.697 kb on + strand at 5537.765 kb on + strand at 5537.765 kb on + strand at 5537.795 kb on - strand at 5537.931 kb on - strand at 5537.981 kb on - strand at 5538.040 kb on + strand, within PP_4871 at 5538.041 kb on - strand, within PP_4871 at 5538.041 kb on - strand, within PP_4871 at 5538.256 kb on - strand, within PP_4871 at 5538.343 kb on + strand, within PP_4871 at 5538.372 kb on + strand, within PP_4871 at 5538.372 kb on + strand, within PP_4871 at 5538.483 kb on + strand at 5538.484 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction PUT 8 MOI 1 remove 5,536,154 + +2.2 5,536,167 + +1.7 5,536,167 + -3.5 5,537,024 + +0.9 5,537,024 + +0.2 5,537,025 - -2.0 5,537,025 - +0.3 5,537,025 - +1.0 5,537,025 - +0.3 5,537,025 - -0.1 5,537,057 + +0.2 5,537,127 + +1.5 5,537,127 + +1.3 5,537,191 - PP_4870 0.14 +0.9 5,537,191 - PP_4870 0.14 -0.2 5,537,301 + PP_4870 0.39 -3.5 5,537,302 - PP_4870 0.39 +0.4 5,537,472 + PP_4870 0.77 +1.0 5,537,473 - PP_4870 0.77 -2.9 5,537,558 + -4.1 5,537,599 + +0.9 5,537,599 + +0.7 5,537,642 + +0.7 5,537,643 - +0.0 5,537,697 + -5.0 5,537,697 + +0.7 5,537,765 + -1.1 5,537,765 + -4.6 5,537,795 - +1.8 5,537,931 - -2.2 5,537,981 - +0.5 5,538,040 + PP_4871 0.20 +0.6 5,538,041 - PP_4871 0.20 +2.2 5,538,041 - PP_4871 0.20 -2.1 5,538,256 - PP_4871 0.57 +2.2 5,538,343 + PP_4871 0.71 +0.8 5,538,372 + PP_4871 0.76 +1.5 5,538,372 + PP_4871 0.76 +0.8 5,538,483 + -2.8 5,538,484 - -2.5
Or see this region's nucleotide sequence