Experiment: PUT 8 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_3988 and PP_3989 are separated by 11 nucleotides PP_3989 and PP_t60 are separated by 254 nucleotides PP_t60 and PP_3990 are separated by 133 nucleotides
PP_3988: PP_3988 - conserved protein of unknown function, at 4,497,955 to 4,500,003
_3988
PP_3989: PP_3989 - DNA-cytosine methyltransferase, at 4,500,015 to 4,501,061
_3989
PP_t60: PP_t60 - tRNA-Ser, at 4,501,316 to 4,501,403
_t60
PP_3990: PP_3990 - substrate or modulator of FtsH-mediated proteolysis, at 4,501,537 to 4,502,208
_3990
Position (kb)
4500
4501
4502 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 4499.015 kb on + strand, within PP_3988 at 4499.015 kb on + strand, within PP_3988 at 4499.037 kb on + strand, within PP_3988 at 4499.121 kb on + strand, within PP_3988 at 4499.121 kb on + strand, within PP_3988 at 4499.128 kb on - strand, within PP_3988 at 4499.213 kb on - strand, within PP_3988 at 4499.674 kb on + strand, within PP_3988 at 4499.721 kb on + strand, within PP_3988 at 4499.908 kb on + strand at 4500.005 kb on + strand at 4500.119 kb on + strand at 4500.173 kb on + strand, within PP_3989 at 4500.302 kb on + strand, within PP_3989 at 4500.679 kb on + strand, within PP_3989 at 4500.725 kb on + strand at 4500.762 kb on + strand, within PP_3989 at 4500.814 kb on + strand, within PP_3989 at 4500.814 kb on + strand, within PP_3989 at 4500.814 kb on + strand, within PP_3989 at 4500.837 kb on + strand, within PP_3989 at 4500.837 kb on + strand, within PP_3989 at 4500.883 kb on + strand, within PP_3989 at 4501.097 kb on - strand at 4501.102 kb on - strand at 4501.269 kb on - strand at 4501.492 kb on - strand at 4501.492 kb on - strand at 4501.492 kb on - strand at 4501.520 kb on + strand at 4501.520 kb on + strand at 4501.521 kb on - strand at 4501.552 kb on + strand at 4501.553 kb on - strand at 4501.615 kb on + strand, within PP_3990 at 4501.615 kb on + strand, within PP_3990 at 4501.616 kb on - strand, within PP_3990 at 4501.616 kb on - strand, within PP_3990 at 4501.632 kb on + strand, within PP_3990 at 4501.710 kb on - strand, within PP_3990 at 4501.720 kb on + strand, within PP_3990 at 4501.720 kb on + strand, within PP_3990 at 4501.721 kb on - strand, within PP_3990 at 4501.738 kb on - strand, within PP_3990 at 4501.859 kb on + strand, within PP_3990 at 4501.860 kb on - strand, within PP_3990 at 4501.912 kb on + strand, within PP_3990 at 4501.912 kb on + strand, within PP_3990 at 4501.912 kb on + strand, within PP_3990 at 4501.913 kb on - strand, within PP_3990 at 4501.913 kb on - strand, within PP_3990 at 4501.913 kb on - strand, within PP_3990 at 4502.035 kb on - strand, within PP_3990
Per-strain Table
Position Strand Gene LocusTag Fraction PUT 8 MOI 1 remove 4,499,015 + PP_3988 0.52 +0.6 4,499,015 + PP_3988 0.52 +0.1 4,499,037 + PP_3988 0.53 +0.4 4,499,121 + PP_3988 0.57 -1.2 4,499,121 + PP_3988 0.57 -3.8 4,499,128 - PP_3988 0.57 +1.1 4,499,213 - PP_3988 0.61 -2.8 4,499,674 + PP_3988 0.84 -1.2 4,499,721 + PP_3988 0.86 +1.2 4,499,908 + +2.2 4,500,005 + -3.9 4,500,119 + -3.3 4,500,173 + PP_3989 0.15 -3.2 4,500,302 + PP_3989 0.27 -0.8 4,500,679 + PP_3989 0.63 -3.0 4,500,725 + -1.0 4,500,762 + PP_3989 0.71 -3.7 4,500,814 + PP_3989 0.76 +1.7 4,500,814 + PP_3989 0.76 -0.0 4,500,814 + PP_3989 0.76 -4.0 4,500,837 + PP_3989 0.79 +0.2 4,500,837 + PP_3989 0.79 -4.4 4,500,883 + PP_3989 0.83 -1.5 4,501,097 - +0.3 4,501,102 - -3.3 4,501,269 - -3.5 4,501,492 - -2.5 4,501,492 - +1.7 4,501,492 - -0.2 4,501,520 + -3.6 4,501,520 + -3.3 4,501,521 - -0.3 4,501,552 + -4.7 4,501,553 - -3.2 4,501,615 + PP_3990 0.12 -3.9 4,501,615 + PP_3990 0.12 -4.3 4,501,616 - PP_3990 0.12 +0.4 4,501,616 - PP_3990 0.12 -1.1 4,501,632 + PP_3990 0.14 -2.6 4,501,710 - PP_3990 0.26 +1.0 4,501,720 + PP_3990 0.27 +0.0 4,501,720 + PP_3990 0.27 +1.5 4,501,721 - PP_3990 0.27 +1.5 4,501,738 - PP_3990 0.30 +0.3 4,501,859 + PP_3990 0.48 -0.5 4,501,860 - PP_3990 0.48 +0.3 4,501,912 + PP_3990 0.56 +1.3 4,501,912 + PP_3990 0.56 +0.4 4,501,912 + PP_3990 0.56 -3.7 4,501,913 - PP_3990 0.56 -3.1 4,501,913 - PP_3990 0.56 -2.2 4,501,913 - PP_3990 0.56 +0.5 4,502,035 - PP_3990 0.74 +1.4
Or see this region's nucleotide sequence