Experiment: PUT 8 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_3187 and PP_3188 are separated by 44 nucleotides PP_3188 and PP_3189 are separated by 4 nucleotides
PP_3187: PP_3187 - Cytosine transporter, at 3,615,666 to 3,616,931
_3187
PP_3188: PP_3188 - conserved protein of unknown function, at 3,616,976 to 3,618,145
_3188
PP_3189: PP_3189 - cytosine deaminase / isoguanine deaminase, at 3,618,150 to 3,619,391
_3189
Position (kb)
3616
3617
3618
3619 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 3615.990 kb on + strand, within PP_3187 at 3615.991 kb on - strand, within PP_3187 at 3616.059 kb on - strand, within PP_3187 at 3616.097 kb on + strand, within PP_3187 at 3616.098 kb on - strand, within PP_3187 at 3616.182 kb on + strand, within PP_3187 at 3616.495 kb on + strand, within PP_3187 at 3616.527 kb on + strand, within PP_3187 at 3616.528 kb on - strand, within PP_3187 at 3616.732 kb on - strand, within PP_3187 at 3616.765 kb on - strand, within PP_3187 at 3616.765 kb on - strand, within PP_3187 at 3616.804 kb on + strand, within PP_3187 at 3616.812 kb on + strand at 3616.867 kb on - strand at 3616.951 kb on - strand at 3616.973 kb on + strand at 3617.027 kb on + strand at 3617.027 kb on + strand at 3617.028 kb on - strand at 3617.028 kb on - strand at 3617.079 kb on + strand at 3617.325 kb on + strand, within PP_3188 at 3617.637 kb on + strand, within PP_3188 at 3617.637 kb on + strand, within PP_3188 at 3617.638 kb on - strand, within PP_3188 at 3617.699 kb on + strand, within PP_3188 at 3617.699 kb on + strand, within PP_3188 at 3617.699 kb on + strand, within PP_3188 at 3617.700 kb on - strand, within PP_3188 at 3617.700 kb on - strand, within PP_3188 at 3617.700 kb on - strand, within PP_3188 at 3617.808 kb on - strand, within PP_3188 at 3617.833 kb on - strand, within PP_3188 at 3617.833 kb on - strand, within PP_3188 at 3617.833 kb on - strand, within PP_3188 at 3617.852 kb on - strand, within PP_3188 at 3617.944 kb on + strand, within PP_3188 at 3617.945 kb on - strand, within PP_3188 at 3618.111 kb on + strand at 3618.112 kb on - strand at 3618.143 kb on + strand at 3618.143 kb on + strand at 3618.144 kb on - strand at 3618.144 kb on - strand at 3618.365 kb on + strand, within PP_3189 at 3618.366 kb on - strand, within PP_3189 at 3618.622 kb on - strand, within PP_3189 at 3618.894 kb on + strand, within PP_3189 at 3618.894 kb on + strand, within PP_3189 at 3618.912 kb on + strand, within PP_3189 at 3618.913 kb on - strand, within PP_3189 at 3619.002 kb on + strand, within PP_3189 at 3619.095 kb on + strand, within PP_3189 at 3619.116 kb on - strand, within PP_3189 at 3619.116 kb on - strand, within PP_3189
Per-strain Table
Position Strand Gene LocusTag Fraction PUT 8 MOI 1 remove 3,615,990 + PP_3187 0.26 -4.0 3,615,991 - PP_3187 0.26 -3.3 3,616,059 - PP_3187 0.31 -0.9 3,616,097 + PP_3187 0.34 -3.9 3,616,098 - PP_3187 0.34 +0.5 3,616,182 + PP_3187 0.41 +0.4 3,616,495 + PP_3187 0.65 +1.1 3,616,527 + PP_3187 0.68 +0.1 3,616,528 - PP_3187 0.68 -3.5 3,616,732 - PP_3187 0.84 +2.6 3,616,765 - PP_3187 0.87 -4.4 3,616,765 - PP_3187 0.87 -0.9 3,616,804 + PP_3187 0.90 -0.6 3,616,812 + -0.3 3,616,867 - +1.0 3,616,951 - +1.1 3,616,973 + -4.8 3,617,027 + -3.6 3,617,027 + +0.2 3,617,028 - -2.8 3,617,028 - -4.1 3,617,079 + -0.5 3,617,325 + PP_3188 0.30 -4.1 3,617,637 + PP_3188 0.56 +0.9 3,617,637 + PP_3188 0.56 -3.3 3,617,638 - PP_3188 0.57 -0.5 3,617,699 + PP_3188 0.62 -1.9 3,617,699 + PP_3188 0.62 -0.7 3,617,699 + PP_3188 0.62 -2.7 3,617,700 - PP_3188 0.62 -4.2 3,617,700 - PP_3188 0.62 -1.1 3,617,700 - PP_3188 0.62 -1.6 3,617,808 - PP_3188 0.71 +0.2 3,617,833 - PP_3188 0.73 -0.0 3,617,833 - PP_3188 0.73 -4.1 3,617,833 - PP_3188 0.73 +0.4 3,617,852 - PP_3188 0.75 -3.6 3,617,944 + PP_3188 0.83 -1.1 3,617,945 - PP_3188 0.83 +0.5 3,618,111 + +0.9 3,618,112 - -1.0 3,618,143 + -0.1 3,618,143 + +0.4 3,618,144 - -0.7 3,618,144 - +0.2 3,618,365 + PP_3189 0.17 -0.0 3,618,366 - PP_3189 0.17 -4.5 3,618,622 - PP_3189 0.38 +0.2 3,618,894 + PP_3189 0.60 +0.2 3,618,894 + PP_3189 0.60 +0.2 3,618,912 + PP_3189 0.61 -1.2 3,618,913 - PP_3189 0.61 -3.8 3,619,002 + PP_3189 0.69 -0.1 3,619,095 + PP_3189 0.76 -0.0 3,619,116 - PP_3189 0.78 +0.8 3,619,116 - PP_3189 0.78 +0.7
Or see this region's nucleotide sequence