Experiment: PUT 8 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_2777 and PP_2778 are separated by 8 nucleotides PP_2778 and PP_2779 are separated by 1 nucleotides
PP_2777: PP_2777 - putative Acyl carrier protein, at 3,162,633 to 3,163,202
_2777
PP_2778: PP_2778 - 3-oxoacyl-(Acyl-carrier-protein) synthase II, at 3,163,211 to 3,164,488
_2778
PP_2779: PP_2779 - putative Beta-ketoacyl synthase, at 3,164,490 to 3,165,572
_2779
Position (kb)
3163
3164
3165 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3 at 3162.290 kb on - strand at 3162.295 kb on - strand at 3162.295 kb on - strand at 3162.389 kb on + strand at 3162.425 kb on - strand at 3162.539 kb on - strand at 3162.556 kb on + strand at 3162.597 kb on + strand at 3162.598 kb on - strand at 3162.619 kb on + strand at 3162.671 kb on + strand at 3162.671 kb on + strand at 3162.672 kb on - strand at 3162.672 kb on - strand at 3162.681 kb on - strand at 3162.708 kb on - strand, within PP_2777 at 3162.734 kb on + strand, within PP_2777 at 3162.735 kb on - strand, within PP_2777 at 3162.801 kb on + strand, within PP_2777 at 3162.933 kb on + strand at 3162.934 kb on - strand, within PP_2777 at 3162.998 kb on + strand, within PP_2777 at 3162.999 kb on - strand, within PP_2777 at 3163.032 kb on + strand, within PP_2777 at 3163.032 kb on + strand, within PP_2777 at 3163.372 kb on + strand, within PP_2778 at 3163.458 kb on - strand, within PP_2778 at 3163.458 kb on - strand, within PP_2778 at 3163.548 kb on - strand, within PP_2778 at 3163.584 kb on - strand, within PP_2778 at 3163.704 kb on + strand, within PP_2778 at 3163.705 kb on - strand, within PP_2778 at 3163.714 kb on + strand, within PP_2778 at 3163.715 kb on - strand, within PP_2778 at 3163.844 kb on + strand, within PP_2778 at 3163.847 kb on + strand, within PP_2778 at 3163.848 kb on - strand, within PP_2778 at 3164.087 kb on + strand, within PP_2778 at 3164.087 kb on + strand, within PP_2778 at 3164.164 kb on + strand, within PP_2778 at 3164.165 kb on - strand, within PP_2778 at 3164.517 kb on + strand at 3164.517 kb on + strand at 3164.518 kb on - strand at 3164.661 kb on + strand, within PP_2779 at 3164.662 kb on - strand, within PP_2779 at 3164.662 kb on - strand, within PP_2779 at 3165.063 kb on - strand, within PP_2779 at 3165.091 kb on - strand, within PP_2779 at 3165.206 kb on + strand, within PP_2779 at 3165.207 kb on - strand, within PP_2779 at 3165.365 kb on + strand, within PP_2779 at 3165.365 kb on + strand, within PP_2779 at 3165.406 kb on + strand, within PP_2779 at 3165.407 kb on - strand, within PP_2779
Per-strain Table
Position Strand Gene LocusTag Fraction PUT 8 MOI 1 remove 3,162,290 - -2.5 3,162,295 - +0.2 3,162,295 - +0.9 3,162,389 + +0.2 3,162,425 - +0.7 3,162,539 - +1.7 3,162,556 + +3.0 3,162,597 + +2.6 3,162,598 - +0.0 3,162,619 + -4.3 3,162,671 + -0.5 3,162,671 + +0.2 3,162,672 - -0.9 3,162,672 - -2.9 3,162,681 - +2.0 3,162,708 - PP_2777 0.13 -2.3 3,162,734 + PP_2777 0.18 +0.7 3,162,735 - PP_2777 0.18 -1.3 3,162,801 + PP_2777 0.29 -0.9 3,162,933 + -0.4 3,162,934 - PP_2777 0.53 +0.3 3,162,998 + PP_2777 0.64 -0.1 3,162,999 - PP_2777 0.64 +0.5 3,163,032 + PP_2777 0.70 +1.5 3,163,032 + PP_2777 0.70 +0.6 3,163,372 + PP_2778 0.13 -1.8 3,163,458 - PP_2778 0.19 -4.7 3,163,458 - PP_2778 0.19 -3.7 3,163,548 - PP_2778 0.26 +0.7 3,163,584 - PP_2778 0.29 -2.2 3,163,704 + PP_2778 0.39 +0.7 3,163,705 - PP_2778 0.39 -4.2 3,163,714 + PP_2778 0.39 +2.0 3,163,715 - PP_2778 0.39 +0.2 3,163,844 + PP_2778 0.50 -0.1 3,163,847 + PP_2778 0.50 -4.0 3,163,848 - PP_2778 0.50 -0.7 3,164,087 + PP_2778 0.69 -4.8 3,164,087 + PP_2778 0.69 -0.6 3,164,164 + PP_2778 0.75 +0.3 3,164,165 - PP_2778 0.75 -0.1 3,164,517 + -0.1 3,164,517 + +0.1 3,164,518 - +0.4 3,164,661 + PP_2779 0.16 -5.2 3,164,662 - PP_2779 0.16 -4.1 3,164,662 - PP_2779 0.16 +0.7 3,165,063 - PP_2779 0.53 -3.4 3,165,091 - PP_2779 0.55 +0.5 3,165,206 + PP_2779 0.66 +1.6 3,165,207 - PP_2779 0.66 -0.8 3,165,365 + PP_2779 0.81 +0.4 3,165,365 + PP_2779 0.81 +0.4 3,165,406 + PP_2779 0.85 -0.3 3,165,407 - PP_2779 0.85 -4.6
Or see this region's nucleotide sequence