Strain Fitness in Pseudomonas putida KT2440 around PP_2532

Experiment: PUT 8 MOI 1

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	PUT 8 MOI 1
2,876,550	+		PP_2531	0.17	+1.4
2,876,570	+		PP_2531	0.19	+2.1
2,876,608	-		PP_2531	0.23	+0.5
2,876,695	+		PP_2531	0.32	+0.9
2,876,696	-		PP_2531	0.32	+0.3
2,876,696	-		PP_2531	0.32	-0.7
2,876,926	+		PP_2531	0.55	-0.4
2,876,926	+		PP_2531	0.55	+1.2
2,876,927	-		PP_2531	0.55	-0.7
2,876,927	-		PP_2531	0.55	+0.0
2,876,927	-		PP_2531	0.55	+2.1
2,876,927	-		PP_2531	0.55	+1.1
2,876,937	-		PP_2531	0.56	+1.3
2,876,943	+		PP_2531	0.57	+0.7
2,876,959	+		PP_2531	0.58	-2.0
2,876,960	-		PP_2531	0.58	-2.6
2,876,960	-		PP_2531	0.58	-0.9
2,877,034	+		PP_2531	0.66	-2.5
2,877,041	-		PP_2531	0.66	+1.1
2,877,088	+		PP_2531	0.71	+0.5
2,877,292	+				+0.2
2,877,409	+				-0.8
2,877,410	-				-3.0
2,877,410	-				+0.4
2,877,410	-				+1.4
2,877,478	-				-1.6
2,877,500	+				-0.3
2,877,527	+				+0.3
2,877,528	-				-0.8
2,877,528	-				-3.8
2,877,681	+		PP_2532	0.34	-4.8
2,877,682	-		PP_2532	0.34	+1.6
2,877,682	-		PP_2532	0.34	+1.2
2,877,682	-		PP_2532	0.34	+0.1
2,877,682	-		PP_2532	0.34	-4.5
2,877,888	+		PP_2532	0.76	+1.7
2,877,889	-		PP_2532	0.77	+1.1
2,877,889	-		PP_2532	0.77	+1.0
2,878,001	-				+0.2
2,878,119	+		PP_2533	0.11	-2.7
2,878,128	+		PP_2533	0.12	-0.7
2,878,147	-		PP_2533	0.14	+1.5
2,878,162	+		PP_2533	0.15	+0.2
2,878,169	+		PP_2533	0.16	-4.2
2,878,169	+		PP_2533	0.16	-2.0
2,878,274	+		PP_2533	0.27	-1.3
2,878,275	-		PP_2533	0.27	+0.7
2,878,406	-		PP_2533	0.40	-2.0
2,878,970	+				-4.9