Strain Fitness in Pseudomonas putida KT2440 around PP_2456

Experiment: PUT 8 MOI 1

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	PUT 8 MOI 1
2,802,235	+		PP_2455	0.39	+0.8
2,802,236	-		PP_2455	0.39	+0.3
2,802,236	-		PP_2455	0.39	-2.5
2,802,236	-		PP_2455	0.39	-0.3
2,802,237	+		PP_2455	0.39	+1.1
2,802,238	-		PP_2455	0.39	+1.1
2,802,332	-		PP_2455	0.45	+0.8
2,802,332	-				-0.7
2,802,384	+		PP_2455	0.49	+1.3
2,802,384	+		PP_2455	0.49	-2.3
2,802,385	-		PP_2455	0.49	-0.4
2,802,547	+		PP_2455	0.59	+1.3
2,802,548	-		PP_2455	0.59	+0.3
2,802,548	-		PP_2455	0.59	+1.8
2,802,958	+		PP_2455	0.85	+1.2
2,802,958	+		PP_2455	0.85	+1.4
2,802,959	-		PP_2455	0.85	+5.2
2,802,959	-		PP_2455	0.85	+1.3
2,803,246	-				+1.5
2,803,263	+				-2.4
2,803,264	-				+1.2
2,803,264	-				-0.1
2,803,335	+		PP_2456	0.15	+0.5
2,803,336	-		PP_2456	0.15	+0.4
2,803,636	-		PP_2456	0.45	+0.9
2,803,662	+		PP_2456	0.48	-3.5
2,803,662	+		PP_2456	0.48	-2.8
2,803,662	+		PP_2456	0.48	+1.3
2,803,663	-		PP_2456	0.48	+1.3
2,803,663	-		PP_2456	0.48	+1.3
2,803,762	+		PP_2456	0.58	+2.9
2,803,851	+		PP_2456	0.67	-3.1
2,803,852	-		PP_2456	0.67	+3.5
2,804,152	-				-0.5
2,804,156	+				-3.0
2,804,230	+				-3.8
2,804,230	+				+2.6
2,804,249	+				+1.2
2,804,249	+				-0.3
2,804,249	+				-0.5
2,804,250	-				+0.5
2,804,250	-				-0.2
2,804,250	-				+0.0
2,804,250	-				-2.0
2,804,456	-		PP_2457	0.26	-3.7
2,804,507	-		PP_2457	0.31	-1.1
2,804,546	+		PP_2457	0.35	-1.3
2,804,680	+		PP_2457	0.48	+1.8
2,804,681	-		PP_2457	0.48	+0.4
2,804,681	-		PP_2457	0.48	-0.0
2,804,728	+		PP_2457	0.53	+0.1
2,804,729	-		PP_2457	0.53	-0.5
2,804,730	+		PP_2457	0.53	+0.4
2,804,730	-		PP_2457	0.53	+1.2
2,804,828	+		PP_2457	0.63	+0.7
2,804,828	+		PP_2457	0.63	-0.7
2,804,829	-				-0.5
2,804,972	+		PP_2457	0.77	-4.7
2,805,022	+		PP_2457	0.82	+0.3
2,805,023	-		PP_2457	0.82	-3.8
2,805,023	-		PP_2457	0.82	-5.1