Experiment: PUT 8 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_2185 and PP_2186 overlap by 4 nucleotides PP_2186 and PP_2187 are separated by 113 nucleotides
PP_2185: PP_2185 - putative Formate dehydrogenase, alpha subunit, at 2,490,578 to 2,493,460
_2185
PP_2186: PP_2186 - putative Formate dehydrogenase, delta subunit, at 2,493,457 to 2,493,660
_2186
PP_2187: PP_2187 - Universal stress protein family, at 2,493,774 to 2,494,637
_2187
Position (kb)
2493
2494 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2492.752 kb on + strand, within PP_2185 at 2492.752 kb on + strand, within PP_2185 at 2492.793 kb on - strand, within PP_2185 at 2492.852 kb on - strand, within PP_2185 at 2492.852 kb on - strand, within PP_2185 at 2492.858 kb on + strand, within PP_2185 at 2492.858 kb on + strand, within PP_2185 at 2492.858 kb on + strand, within PP_2185 at 2492.859 kb on - strand, within PP_2185 at 2493.054 kb on - strand, within PP_2185 at 2493.242 kb on + strand at 2493.242 kb on + strand at 2493.242 kb on + strand at 2493.243 kb on - strand at 2493.243 kb on - strand at 2493.502 kb on + strand, within PP_2186 at 2493.502 kb on + strand, within PP_2186 at 2493.503 kb on - strand at 2493.503 kb on - strand, within PP_2186 at 2493.503 kb on - strand, within PP_2186 at 2493.659 kb on - strand at 2493.749 kb on + strand at 2493.750 kb on - strand at 2493.764 kb on + strand at 2493.765 kb on - strand at 2493.772 kb on - strand at 2493.855 kb on + strand at 2493.855 kb on + strand at 2493.856 kb on - strand at 2493.872 kb on + strand, within PP_2187 at 2493.950 kb on + strand, within PP_2187 at 2494.012 kb on + strand, within PP_2187 at 2494.392 kb on + strand, within PP_2187 at 2494.392 kb on + strand, within PP_2187 at 2494.393 kb on - strand, within PP_2187 at 2494.393 kb on - strand, within PP_2187 at 2494.393 kb on - strand, within PP_2187 at 2494.393 kb on - strand, within PP_2187 at 2494.575 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction PUT 8 MOI 1 remove 2,492,752 + PP_2185 0.75 +1.1 2,492,752 + PP_2185 0.75 +0.2 2,492,793 - PP_2185 0.77 -0.8 2,492,852 - PP_2185 0.79 +0.9 2,492,852 - PP_2185 0.79 +0.2 2,492,858 + PP_2185 0.79 +2.6 2,492,858 + PP_2185 0.79 +1.0 2,492,858 + PP_2185 0.79 -1.9 2,492,859 - PP_2185 0.79 -1.2 2,493,054 - PP_2185 0.86 -0.2 2,493,242 + -4.6 2,493,242 + +0.7 2,493,242 + +0.2 2,493,243 - -2.6 2,493,243 - +0.5 2,493,502 + PP_2186 0.22 -1.0 2,493,502 + PP_2186 0.22 -2.9 2,493,503 - +0.8 2,493,503 - PP_2186 0.23 -2.0 2,493,503 - PP_2186 0.23 -0.5 2,493,659 - -3.7 2,493,749 + +0.5 2,493,750 - +0.6 2,493,764 + +1.0 2,493,765 - -0.1 2,493,772 - +2.3 2,493,855 + -3.2 2,493,855 + -2.8 2,493,856 - -0.6 2,493,872 + PP_2187 0.11 -1.8 2,493,950 + PP_2187 0.20 -3.2 2,494,012 + PP_2187 0.28 +0.6 2,494,392 + PP_2187 0.72 -0.6 2,494,392 + PP_2187 0.72 +0.7 2,494,393 - PP_2187 0.72 +0.8 2,494,393 - PP_2187 0.72 +0.6 2,494,393 - PP_2187 0.72 -2.8 2,494,393 - PP_2187 0.72 +1.5 2,494,575 - -2.8
Or see this region's nucleotide sequence