Experiment: PUT 8 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_2029 and PP_2030 overlap by 8 nucleotides PP_2030 and PP_2031 overlap by 4 nucleotides PP_2031 and PP_2032 overlap by 8 nucleotides
PP_2029: PP_2029 - von Willebrand factor type A domain protein, at 2,306,553 to 2,307,629
_2029
PP_2030: PP_2030 - conserved protein of unknown function, at 2,307,622 to 2,308,116
_2030
PP_2031: PP_2031 - conserved protein of unknown function, at 2,308,113 to 2,309,069
_2031
PP_2032: PP_2032 - putative ATPase, at 2,309,062 to 2,310,021
_2032
Position (kb)
2308
2309
2310 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3 at 2307.193 kb on + strand at 2307.193 kb on + strand, within PP_2029 at 2307.704 kb on + strand, within PP_2030 at 2307.705 kb on - strand, within PP_2030 at 2307.715 kb on + strand, within PP_2030 at 2307.715 kb on + strand, within PP_2030 at 2307.715 kb on + strand, within PP_2030 at 2307.715 kb on + strand, within PP_2030 at 2307.715 kb on + strand, within PP_2030 at 2307.716 kb on - strand, within PP_2030 at 2307.716 kb on - strand at 2307.722 kb on - strand, within PP_2030 at 2307.722 kb on - strand, within PP_2030 at 2307.887 kb on + strand, within PP_2030 at 2308.827 kb on - strand, within PP_2031 at 2308.877 kb on - strand, within PP_2031 at 2308.877 kb on - strand, within PP_2031 at 2309.348 kb on + strand, within PP_2032 at 2309.348 kb on + strand, within PP_2032 at 2309.372 kb on + strand, within PP_2032 at 2309.372 kb on + strand, within PP_2032 at 2309.372 kb on + strand, within PP_2032 at 2309.373 kb on - strand, within PP_2032 at 2309.373 kb on - strand, within PP_2032 at 2309.488 kb on + strand, within PP_2032 at 2309.516 kb on - strand at 2309.558 kb on + strand, within PP_2032 at 2309.559 kb on - strand, within PP_2032 at 2309.621 kb on + strand, within PP_2032 at 2309.622 kb on - strand, within PP_2032 at 2309.622 kb on - strand, within PP_2032 at 2309.622 kb on - strand, within PP_2032 at 2309.725 kb on - strand, within PP_2032 at 2309.733 kb on + strand, within PP_2032 at 2309.733 kb on + strand, within PP_2032 at 2309.734 kb on - strand, within PP_2032 at 2309.965 kb on + strand at 2309.965 kb on + strand at 2309.965 kb on + strand at 2309.977 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction PUT 8 MOI 1 remove 2,307,193 + +0.8 2,307,193 + PP_2029 0.59 -3.3 2,307,704 + PP_2030 0.17 -0.2 2,307,705 - PP_2030 0.17 -3.6 2,307,715 + PP_2030 0.19 -3.1 2,307,715 + PP_2030 0.19 -2.5 2,307,715 + PP_2030 0.19 -3.8 2,307,715 + PP_2030 0.19 -3.8 2,307,715 + PP_2030 0.19 -2.6 2,307,716 - PP_2030 0.19 -4.6 2,307,716 - -1.6 2,307,722 - PP_2030 0.20 -1.0 2,307,722 - PP_2030 0.20 -3.9 2,307,887 + PP_2030 0.54 -0.1 2,308,827 - PP_2031 0.75 -4.0 2,308,877 - PP_2031 0.80 -3.8 2,308,877 - PP_2031 0.80 -3.5 2,309,348 + PP_2032 0.30 -2.9 2,309,348 + PP_2032 0.30 -4.3 2,309,372 + PP_2032 0.32 -4.0 2,309,372 + PP_2032 0.32 -3.6 2,309,372 + PP_2032 0.32 -3.3 2,309,373 - PP_2032 0.32 -4.0 2,309,373 - PP_2032 0.32 -3.5 2,309,488 + PP_2032 0.44 -4.9 2,309,516 - +3.7 2,309,558 + PP_2032 0.52 -0.6 2,309,559 - PP_2032 0.52 -4.3 2,309,621 + PP_2032 0.58 -2.5 2,309,622 - PP_2032 0.58 -2.3 2,309,622 - PP_2032 0.58 -1.9 2,309,622 - PP_2032 0.58 -3.3 2,309,725 - PP_2032 0.69 -2.7 2,309,733 + PP_2032 0.70 +0.2 2,309,733 + PP_2032 0.70 +0.6 2,309,734 - PP_2032 0.70 -1.0 2,309,965 + -4.1 2,309,965 + -4.1 2,309,965 + -3.5 2,309,977 - -3.1
Or see this region's nucleotide sequence