Experiment: PUT 8 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_5497 and PP_2026 are separated by 164 nucleotides PP_2026 and PP_2027 are separated by 438 nucleotides PP_2027 and PP_2028 overlap by 4 nucleotides
PP_5497: PP_5497 - conserved protein of unknown function with rhs repeat-associated core domain, at 2,301,687 to 2,302,340
_5497
PP_2026: PP_2026 - conserved protein of unknown function, at 2,302,505 to 2,302,771
_2026
PP_2027: PP_2027 - conserved exported protein of unknown function, at 2,303,210 to 2,304,841
_2027
PP_2028: PP_2028 - conserved protein of unknown function containing tetratricopeptide (TPR) domain repeat, at 2,304,838 to 2,306,556
_2028
Position (kb)
2303
2304
2305 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3 at 2302.338 kb on + strand at 2302.539 kb on - strand, within PP_2026 at 2302.632 kb on + strand, within PP_2026 at 2302.632 kb on + strand, within PP_2026 at 2302.633 kb on - strand, within PP_2026 at 2302.633 kb on - strand, within PP_2026 at 2302.633 kb on - strand, within PP_2026 at 2302.719 kb on + strand, within PP_2026 at 2302.719 kb on + strand, within PP_2026 at 2302.719 kb on + strand, within PP_2026 at 2302.720 kb on - strand, within PP_2026 at 2302.720 kb on - strand, within PP_2026 at 2302.776 kb on + strand at 2302.867 kb on + strand at 2302.867 kb on + strand at 2302.903 kb on + strand at 2302.904 kb on - strand at 2302.915 kb on + strand at 2302.928 kb on + strand at 2302.929 kb on - strand at 2303.011 kb on + strand at 2303.011 kb on + strand at 2303.012 kb on - strand at 2303.012 kb on - strand at 2303.082 kb on + strand at 2303.083 kb on - strand at 2303.087 kb on + strand at 2303.087 kb on + strand at 2303.088 kb on - strand at 2303.088 kb on - strand at 2303.088 kb on - strand at 2303.096 kb on - strand at 2303.211 kb on + strand at 2303.211 kb on + strand at 2303.211 kb on + strand at 2303.212 kb on - strand at 2303.284 kb on + strand at 2303.284 kb on + strand at 2303.319 kb on + strand at 2303.319 kb on + strand at 2303.319 kb on + strand at 2303.337 kb on + strand at 2303.338 kb on - strand at 2303.985 kb on + strand, within PP_2027 at 2303.985 kb on - strand, within PP_2027 at 2304.234 kb on + strand, within PP_2027 at 2304.234 kb on + strand, within PP_2027 at 2304.235 kb on - strand, within PP_2027 at 2304.363 kb on + strand, within PP_2027 at 2304.364 kb on - strand, within PP_2027 at 2304.382 kb on - strand, within PP_2027 at 2304.418 kb on - strand, within PP_2027 at 2304.418 kb on - strand, within PP_2027 at 2304.418 kb on - strand, within PP_2027 at 2304.452 kb on + strand, within PP_2027 at 2304.452 kb on + strand, within PP_2027 at 2304.453 kb on - strand, within PP_2027 at 2304.453 kb on - strand, within PP_2027 at 2304.453 kb on - strand, within PP_2027 at 2304.577 kb on + strand, within PP_2027 at 2304.577 kb on + strand, within PP_2027 at 2304.872 kb on + strand at 2305.280 kb on + strand, within PP_2028 at 2305.281 kb on - strand, within PP_2028 at 2305.338 kb on - strand, within PP_2028 at 2305.389 kb on - strand, within PP_2028 at 2305.832 kb on + strand at 2305.833 kb on - strand, within PP_2028
Per-strain Table
Position Strand Gene LocusTag Fraction PUT 8 MOI 1 remove 2,302,338 + -0.1 2,302,539 - PP_2026 0.13 -1.4 2,302,632 + PP_2026 0.48 -0.9 2,302,632 + PP_2026 0.48 -1.3 2,302,633 - PP_2026 0.48 +1.7 2,302,633 - PP_2026 0.48 -1.5 2,302,633 - PP_2026 0.48 -4.9 2,302,719 + PP_2026 0.80 -0.1 2,302,719 + PP_2026 0.80 -4.6 2,302,719 + PP_2026 0.80 -2.0 2,302,720 - PP_2026 0.81 -1.4 2,302,720 - PP_2026 0.81 +0.8 2,302,776 + -0.0 2,302,867 + +0.8 2,302,867 + -0.3 2,302,903 + +2.0 2,302,904 - -4.1 2,302,915 + -4.1 2,302,928 + -4.3 2,302,929 - +0.7 2,303,011 + +0.5 2,303,011 + +1.8 2,303,012 - +1.3 2,303,012 - +1.8 2,303,082 + +0.4 2,303,083 - -0.7 2,303,087 + +1.7 2,303,087 + -0.4 2,303,088 - +0.8 2,303,088 - -0.7 2,303,088 - -0.7 2,303,096 - -0.4 2,303,211 + +0.1 2,303,211 + -1.4 2,303,211 + -0.7 2,303,212 - -0.4 2,303,284 + -4.4 2,303,284 + -3.5 2,303,319 + -3.8 2,303,319 + -0.2 2,303,319 + -3.2 2,303,337 + -2.9 2,303,338 - +0.9 2,303,985 + PP_2027 0.47 -4.5 2,303,985 - PP_2027 0.47 -0.4 2,304,234 + PP_2027 0.63 -4.7 2,304,234 + PP_2027 0.63 -3.6 2,304,235 - PP_2027 0.63 -4.1 2,304,363 + PP_2027 0.71 -3.4 2,304,364 - PP_2027 0.71 -2.8 2,304,382 - PP_2027 0.72 +0.0 2,304,418 - PP_2027 0.74 -3.6 2,304,418 - PP_2027 0.74 +1.0 2,304,418 - PP_2027 0.74 -2.6 2,304,452 + PP_2027 0.76 +0.1 2,304,452 + PP_2027 0.76 -2.9 2,304,453 - PP_2027 0.76 -1.6 2,304,453 - PP_2027 0.76 -4.5 2,304,453 - PP_2027 0.76 -2.0 2,304,577 + PP_2027 0.84 -3.9 2,304,577 + PP_2027 0.84 -3.9 2,304,872 + -3.7 2,305,280 + PP_2028 0.26 -4.2 2,305,281 - PP_2028 0.26 -3.1 2,305,338 - PP_2028 0.29 -3.4 2,305,389 - PP_2028 0.32 +2.9 2,305,832 + +1.8 2,305,833 - PP_2028 0.58 -2.1
Or see this region's nucleotide sequence