Experiment: PUT 8 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_1868 and PP_1869 are separated by 90 nucleotides PP_1869 and PP_1870 are separated by 74 nucleotides PP_1870 and PP_1871 are separated by 97 nucleotides
PP_1868: PP_1868 - ATP-dependent DEAD-box RNA helicase DeaD, at 2,090,675 to 2,092,354
_1868
PP_1869: PP_1869 - 4,5-DOPA-dioxygenase, at 2,092,445 to 2,093,239
_1869
PP_1870: PP_1870 - putative thiopurine S-methyltransferase, at 2,093,314 to 2,093,964
_1870
PP_1871: PP_1871 - Protease HtpX, at 2,094,062 to 2,094,949
_1871
Position (kb)
2092
2093
2094 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 2092.458 kb on + strand at 2092.458 kb on + strand at 2092.459 kb on - strand at 2092.459 kb on - strand at 2092.570 kb on + strand, within PP_1869 at 2092.570 kb on + strand, within PP_1869 at 2092.723 kb on + strand, within PP_1869 at 2092.739 kb on + strand, within PP_1869 at 2092.739 kb on + strand, within PP_1869 at 2092.740 kb on - strand, within PP_1869 at 2092.740 kb on - strand, within PP_1869 at 2092.740 kb on - strand, within PP_1869 at 2092.742 kb on + strand, within PP_1869 at 2092.742 kb on + strand, within PP_1869 at 2092.748 kb on + strand, within PP_1869 at 2092.748 kb on + strand, within PP_1869 at 2092.748 kb on + strand, within PP_1869 at 2092.748 kb on + strand, within PP_1869 at 2092.748 kb on + strand, within PP_1869 at 2092.749 kb on - strand, within PP_1869 at 2092.749 kb on - strand, within PP_1869 at 2092.749 kb on - strand, within PP_1869 at 2092.749 kb on - strand, within PP_1869 at 2092.749 kb on - strand, within PP_1869 at 2092.749 kb on - strand, within PP_1869 at 2092.749 kb on - strand, within PP_1869 at 2092.857 kb on + strand at 2092.998 kb on + strand, within PP_1869 at 2092.998 kb on + strand, within PP_1869 at 2092.999 kb on - strand, within PP_1869 at 2093.020 kb on - strand, within PP_1869 at 2093.230 kb on + strand at 2093.230 kb on + strand at 2093.251 kb on + strand at 2093.256 kb on - strand at 2093.256 kb on - strand at 2093.386 kb on + strand, within PP_1870 at 2093.387 kb on - strand, within PP_1870 at 2093.398 kb on + strand, within PP_1870 at 2093.398 kb on + strand, within PP_1870 at 2093.399 kb on - strand, within PP_1870 at 2093.498 kb on + strand, within PP_1870 at 2093.498 kb on + strand, within PP_1870 at 2093.666 kb on + strand, within PP_1870 at 2093.674 kb on + strand, within PP_1870 at 2093.674 kb on + strand, within PP_1870 at 2093.675 kb on - strand, within PP_1870 at 2093.722 kb on + strand, within PP_1870 at 2093.722 kb on + strand, within PP_1870 at 2093.722 kb on + strand, within PP_1870 at 2093.723 kb on - strand at 2093.723 kb on - strand, within PP_1870 at 2093.723 kb on - strand, within PP_1870 at 2093.724 kb on + strand, within PP_1870 at 2093.724 kb on + strand, within PP_1870 at 2093.725 kb on - strand, within PP_1870 at 2093.725 kb on - strand, within PP_1870 at 2093.947 kb on + strand at 2093.948 kb on - strand at 2093.948 kb on - strand at 2093.970 kb on + strand at 2093.971 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction PUT 8 MOI 1 remove 2,092,458 + -2.6 2,092,458 + -2.3 2,092,459 - -2.2 2,092,459 - -4.3 2,092,570 + PP_1869 0.16 -0.1 2,092,570 + PP_1869 0.16 +1.6 2,092,723 + PP_1869 0.35 -0.1 2,092,739 + PP_1869 0.37 +0.2 2,092,739 + PP_1869 0.37 +1.0 2,092,740 - PP_1869 0.37 +0.6 2,092,740 - PP_1869 0.37 -3.6 2,092,740 - PP_1869 0.37 -0.3 2,092,742 + PP_1869 0.37 -3.9 2,092,742 + PP_1869 0.37 +0.4 2,092,748 + PP_1869 0.38 -4.2 2,092,748 + PP_1869 0.38 -4.4 2,092,748 + PP_1869 0.38 +0.3 2,092,748 + PP_1869 0.38 -0.5 2,092,748 + PP_1869 0.38 +0.4 2,092,749 - PP_1869 0.38 -0.2 2,092,749 - PP_1869 0.38 +0.5 2,092,749 - PP_1869 0.38 -0.4 2,092,749 - PP_1869 0.38 +0.7 2,092,749 - PP_1869 0.38 +0.5 2,092,749 - PP_1869 0.38 +0.5 2,092,749 - PP_1869 0.38 +1.6 2,092,857 + -0.2 2,092,998 + PP_1869 0.70 +0.9 2,092,998 + PP_1869 0.70 +1.4 2,092,999 - PP_1869 0.70 -1.5 2,093,020 - PP_1869 0.72 +0.4 2,093,230 + +1.0 2,093,230 + +0.8 2,093,251 + -3.6 2,093,256 - +0.6 2,093,256 - -0.5 2,093,386 + PP_1870 0.11 -0.1 2,093,387 - PP_1870 0.11 +0.4 2,093,398 + PP_1870 0.13 -4.3 2,093,398 + PP_1870 0.13 +0.1 2,093,399 - PP_1870 0.13 -1.8 2,093,498 + PP_1870 0.28 -0.7 2,093,498 + PP_1870 0.28 +0.1 2,093,666 + PP_1870 0.54 +0.1 2,093,674 + PP_1870 0.55 -3.6 2,093,674 + PP_1870 0.55 +1.4 2,093,675 - PP_1870 0.55 +0.9 2,093,722 + PP_1870 0.63 -3.9 2,093,722 + PP_1870 0.63 -3.6 2,093,722 + PP_1870 0.63 +1.1 2,093,723 - -0.2 2,093,723 - PP_1870 0.63 +3.3 2,093,723 - PP_1870 0.63 +1.0 2,093,724 + PP_1870 0.63 -0.1 2,093,724 + PP_1870 0.63 -3.9 2,093,725 - PP_1870 0.63 -1.8 2,093,725 - PP_1870 0.63 +1.5 2,093,947 + -1.0 2,093,948 - +0.2 2,093,948 - +1.7 2,093,970 + -0.1 2,093,971 - +2.1
Or see this region's nucleotide sequence