Strain Fitness in Pseudomonas putida KT2440 around PP_1750

Experiment: PUT 8 MOI 1

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	PUT 8 MOI 1
1,948,829	-		PP_1749	0.53	-3.5
1,948,936	+		PP_1749	0.59	+0.3
1,948,985	-		PP_1749	0.62	+1.0
1,948,985	-		PP_1749	0.62	-3.5
1,949,183	-		PP_1749	0.73	+1.3
1,949,210	+		PP_1749	0.75	+0.2
1,949,252	-		PP_1749	0.77	+0.0
1,949,316	+				+1.1
1,949,425	+		PP_1749	0.87	-0.9
1,949,426	-		PP_1749	0.87	+0.1
1,949,426	-		PP_1749	0.87	-0.4
1,949,426	-		PP_1749	0.87	-0.3
1,949,506	-				+0.9
1,949,506	-				-3.9
1,949,632	+				-0.4
1,949,872	+		PP_1750	0.12	+0.7
1,949,872	+		PP_1750	0.12	+0.5
1,949,873	-		PP_1750	0.12	+0.0
1,949,873	-		PP_1750	0.12	-0.9
1,949,999	-		PP_1750	0.19	-3.6
1,950,208	+		PP_1750	0.31	-0.9
1,950,254	-		PP_1750	0.33	+1.5
1,950,254	-		PP_1750	0.33	+0.4
1,950,254	-		PP_1750	0.33	-0.5
1,950,254	-		PP_1750	0.33	+0.7
1,950,330	+		PP_1750	0.38	+0.8
1,950,331	-		PP_1750	0.38	-0.4
1,950,370	+		PP_1750	0.40	+1.2
1,950,370	+		PP_1750	0.40	-2.5
1,950,370	+		PP_1750	0.40	+0.1
1,950,371	-		PP_1750	0.40	+1.9
1,950,371	-		PP_1750	0.40	+0.8
1,950,371	-		PP_1750	0.40	+0.3
1,950,484	+		PP_1750	0.46	+1.4
1,950,484	+		PP_1750	0.46	-3.5
1,950,484	+		PP_1750	0.46	-2.8
1,950,751	+		PP_1750	0.61	+0.7
1,950,884	-		PP_1750	0.69	+0.2
1,951,153	+		PP_1750	0.84	+1.2
1,951,154	-		PP_1750	0.84	+2.8
1,951,154	-		PP_1750	0.84	-3.7
1,951,196	-		PP_1750	0.86	+1.5
1,951,196	-		PP_1750	0.86	+1.1
1,951,196	-		PP_1750	0.86	-3.2
1,951,232	+				+0.3
1,951,233	-		PP_1750	0.88	+0.2
1,951,233	-		PP_1750	0.88	+0.9
1,951,317	-				+1.0
1,951,422	+				-4.1
1,951,422	+				+1.2
1,951,422	+				+0.6
1,951,422	+				-4.1
1,951,434	+				-0.3
1,951,445	+				-3.4
1,951,445	+				-0.6
1,951,445	+				+2.1
1,951,445	+				-2.5
1,951,445	+				+0.6
1,951,445	+				+0.0
1,951,446	-				-1.2
1,951,446	-				+1.5
1,951,446	-				+0.5
1,951,446	-				-0.6
1,951,446	-				+1.9
1,951,548	-				+0.1
1,951,548	-				+2.0
1,951,666	+				+0.1
1,951,666	+				+0.3
1,951,739	+				+1.7
1,951,739	+				-1.8
1,951,739	+				+0.4
1,951,739	+				-1.9
1,951,740	-				-2.8
1,951,740	-				-3.6
1,951,740	-				+1.0
1,951,740	-				-3.1
1,951,740	-				-2.9
1,951,740	-				+0.2
1,951,740	-				-3.3
1,951,774	+		PP_1751	0.10	+1.2
1,951,775	-		PP_1751	0.10	-0.3
1,951,799	-		PP_1751	0.11	-4.2
1,951,805	+		PP_1751	0.12	-2.7
1,951,805	+		PP_1751	0.12	-0.1
1,951,806	-		PP_1751	0.12	-2.9
1,951,806	-		PP_1751	0.12	+0.3
1,951,806	-		PP_1751	0.12	+0.9
1,951,806	-		PP_1751	0.12	+0.5
1,951,806	-		PP_1751	0.12	+2.1
1,952,037	+		PP_1751	0.23	-0.5
1,952,037	+		PP_1751	0.23	+1.2
1,952,037	+		PP_1751	0.23	-3.6
1,952,037	+		PP_1751	0.23	+1.5
1,952,038	-		PP_1751	0.24	-2.0
1,952,066	+		PP_1751	0.25	-3.6
1,952,066	+		PP_1751	0.25	-4.1
1,952,066	+		PP_1751	0.25	+0.7
1,952,066	+		PP_1751	0.25	+1.8
1,952,066	+		PP_1751	0.25	+0.3
1,952,067	-		PP_1751	0.25	-2.9
1,952,067	-		PP_1751	0.25	-0.2
1,952,067	-		PP_1751	0.25	-0.1
1,952,155	+		PP_1751	0.29	+0.6
1,952,155	+		PP_1751	0.29	+1.8
1,952,225	-		PP_1751	0.33	-0.1
1,952,307	+		PP_1751	0.37	-0.0
1,952,370	-		PP_1751	0.40	+0.4