Experiment: PUT 8 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_1182 and PP_1183 are separated by 3 nucleotides PP_1183 and PP_1184 are separated by 81 nucleotides PP_1184 and PP_1185 are separated by 123 nucleotides PP_1185 and PP_1186 are separated by 196 nucleotides
PP_1182: PP_1182 - Sensor histidine kinase, at 1,356,820 to 1,358,427
_1182
PP_1183: PP_1183 - putative Siderophore biosynthesis protein, at 1,358,431 to 1,359,132
_1183
PP_1184: PP_1184 - Dienelactone hydrolase family protein, at 1,359,214 to 1,360,011
_1184
PP_1185: PP_1185 - Outer membrane protein H1, at 1,360,135 to 1,360,740
_1185
PP_1186: PP_1186 - two component system DNA-binding transcriptional dual regulator, at 1,360,937 to 1,361,614
_1186
Position (kb)
1359
1360
1361 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3 at 1358.278 kb on - strand at 1358.278 kb on - strand at 1358.333 kb on - strand at 1358.425 kb on + strand at 1358.426 kb on - strand at 1359.140 kb on - strand at 1359.215 kb on + strand at 1359.246 kb on + strand at 1359.246 kb on + strand at 1359.246 kb on + strand at 1359.247 kb on - strand at 1359.247 kb on - strand at 1359.247 kb on - strand at 1359.279 kb on + strand at 1359.280 kb on - strand at 1359.280 kb on - strand at 1359.281 kb on + strand at 1359.282 kb on - strand at 1359.363 kb on + strand, within PP_1184 at 1359.377 kb on + strand, within PP_1184 at 1359.378 kb on - strand, within PP_1184 at 1359.378 kb on - strand, within PP_1184 at 1359.408 kb on + strand, within PP_1184 at 1359.409 kb on - strand, within PP_1184 at 1359.752 kb on + strand, within PP_1184 at 1359.752 kb on + strand, within PP_1184 at 1359.753 kb on - strand, within PP_1184 at 1359.753 kb on - strand, within PP_1184 at 1359.753 kb on - strand, within PP_1184 at 1359.753 kb on - strand, within PP_1184 at 1359.753 kb on - strand, within PP_1184 at 1359.885 kb on - strand, within PP_1184 at 1359.885 kb on - strand, within PP_1184 at 1359.890 kb on + strand at 1359.947 kb on - strand at 1360.086 kb on + strand at 1360.086 kb on + strand at 1360.087 kb on - strand at 1360.091 kb on + strand at 1360.092 kb on - strand at 1360.092 kb on - strand at 1360.127 kb on - strand at 1360.231 kb on + strand, within PP_1185 at 1360.231 kb on + strand, within PP_1185 at 1360.232 kb on - strand, within PP_1185 at 1360.304 kb on + strand, within PP_1185 at 1360.306 kb on - strand, within PP_1185 at 1360.306 kb on - strand, within PP_1185 at 1360.324 kb on - strand, within PP_1185 at 1360.369 kb on + strand, within PP_1185 at 1360.369 kb on + strand, within PP_1185 at 1360.369 kb on + strand, within PP_1185 at 1360.378 kb on + strand, within PP_1185 at 1360.378 kb on + strand, within PP_1185 at 1360.378 kb on + strand, within PP_1185 at 1360.379 kb on - strand, within PP_1185 at 1360.379 kb on - strand, within PP_1185 at 1360.379 kb on - strand, within PP_1185 at 1360.379 kb on - strand at 1360.467 kb on + strand, within PP_1185 at 1360.550 kb on - strand, within PP_1185 at 1360.562 kb on - strand, within PP_1185 at 1360.621 kb on + strand, within PP_1185 at 1360.628 kb on - strand, within PP_1185 at 1360.714 kb on + strand at 1360.714 kb on + strand at 1360.729 kb on + strand at 1360.729 kb on + strand at 1360.730 kb on - strand at 1360.730 kb on - strand at 1360.762 kb on + strand at 1360.848 kb on + strand at 1360.848 kb on + strand at 1360.849 kb on - strand at 1360.849 kb on - strand at 1360.874 kb on + strand at 1360.875 kb on - strand at 1360.918 kb on + strand at 1360.918 kb on + strand at 1360.918 kb on + strand at 1360.918 kb on + strand at 1360.918 kb on + strand at 1360.918 kb on + strand at 1360.918 kb on + strand at 1360.919 kb on - strand at 1360.919 kb on - strand at 1360.924 kb on + strand at 1360.924 kb on + strand at 1360.924 kb on + strand at 1360.925 kb on - strand at 1360.925 kb on - strand at 1360.925 kb on - strand at 1360.925 kb on - strand at 1360.986 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction PUT 8 MOI 1 remove 1,358,278 - +0.7 1,358,278 - -3.5 1,358,333 - -0.9 1,358,425 + -3.3 1,358,426 - -0.3 1,359,140 - -3.3 1,359,215 + +1.6 1,359,246 + -0.6 1,359,246 + -0.1 1,359,246 + +1.3 1,359,247 - -2.9 1,359,247 - -3.5 1,359,247 - +0.3 1,359,279 + -0.3 1,359,280 - -4.1 1,359,280 - -0.6 1,359,281 + +1.8 1,359,282 - +1.0 1,359,363 + PP_1184 0.19 -0.5 1,359,377 + PP_1184 0.20 -0.3 1,359,378 - PP_1184 0.21 -1.2 1,359,378 - PP_1184 0.21 -3.8 1,359,408 + PP_1184 0.24 -4.3 1,359,409 - PP_1184 0.24 -2.0 1,359,752 + PP_1184 0.67 -0.9 1,359,752 + PP_1184 0.67 -0.3 1,359,753 - PP_1184 0.68 -0.6 1,359,753 - PP_1184 0.68 +1.1 1,359,753 - PP_1184 0.68 -5.3 1,359,753 - PP_1184 0.68 +1.9 1,359,753 - PP_1184 0.68 +1.9 1,359,885 - PP_1184 0.84 -4.4 1,359,885 - PP_1184 0.84 +0.0 1,359,890 + +0.3 1,359,947 - +0.5 1,360,086 + +1.3 1,360,086 + +1.2 1,360,087 - -1.9 1,360,091 + +0.1 1,360,092 - -0.0 1,360,092 - +2.4 1,360,127 - +1.4 1,360,231 + PP_1185 0.16 +1.6 1,360,231 + PP_1185 0.16 -4.4 1,360,232 - PP_1185 0.16 -2.8 1,360,304 + PP_1185 0.28 +1.6 1,360,306 - PP_1185 0.28 +1.8 1,360,306 - PP_1185 0.28 -2.6 1,360,324 - PP_1185 0.31 +3.1 1,360,369 + PP_1185 0.39 +0.4 1,360,369 + PP_1185 0.39 +0.6 1,360,369 + PP_1185 0.39 +1.0 1,360,378 + PP_1185 0.40 +1.2 1,360,378 + PP_1185 0.40 +0.4 1,360,378 + PP_1185 0.40 -2.4 1,360,379 - PP_1185 0.40 -2.8 1,360,379 - PP_1185 0.40 +1.9 1,360,379 - PP_1185 0.40 -1.7 1,360,379 - -0.1 1,360,467 + PP_1185 0.55 -3.5 1,360,550 - PP_1185 0.68 +1.4 1,360,562 - PP_1185 0.70 -0.4 1,360,621 + PP_1185 0.80 +1.2 1,360,628 - PP_1185 0.81 -0.7 1,360,714 + -1.4 1,360,714 + -2.7 1,360,729 + +2.0 1,360,729 + -1.4 1,360,730 - -2.0 1,360,730 - -1.7 1,360,762 + -0.7 1,360,848 + -0.2 1,360,848 + +1.7 1,360,849 - -0.1 1,360,849 - +3.0 1,360,874 + +0.9 1,360,875 - -1.3 1,360,918 + +1.0 1,360,918 + -1.5 1,360,918 + -3.2 1,360,918 + -2.7 1,360,918 + -3.1 1,360,918 + -0.6 1,360,918 + -5.3 1,360,919 - -3.4 1,360,919 - -2.9 1,360,924 + -0.5 1,360,924 + -0.4 1,360,924 + -1.0 1,360,925 - -3.3 1,360,925 - -1.7 1,360,925 - -1.6 1,360,925 - -0.2 1,360,986 - -1.1
Or see this region's nucleotide sequence