Experiment: PUT 8 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0264 and PP_0265 are separated by 42 nucleotides PP_0265 and PP_0266 are separated by 277 nucleotides
PP_0264: PP_0264 - Sensor histidine kinase, at 317,927 to 319,741
_0264
PP_0265: PP_0265 - dTDP-4-dehydrorhamnose 3,5-epimerase, at 319,784 to 320,329
_0265
PP_0266: PP_0266 - Agmatine deiminase, at 320,607 to 321,713
_0266
Position (kb) 319
320
321 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4
5 at 318.816 kb on + strand, within PP_0264 at 318.816 kb on + strand, within PP_0264 at 318.816 kb on + strand, within PP_0264 at 318.817 kb on - strand, within PP_0264 at 318.817 kb on - strand, within PP_0264 at 318.860 kb on + strand, within PP_0264 at 318.861 kb on - strand, within PP_0264 at 318.890 kb on + strand, within PP_0264 at 319.191 kb on - strand, within PP_0264 at 319.324 kb on - strand, within PP_0264 at 319.324 kb on - strand, within PP_0264 at 319.413 kb on + strand, within PP_0264 at 319.413 kb on + strand, within PP_0264 at 319.414 kb on - strand, within PP_0264 at 319.414 kb on - strand, within PP_0264 at 319.414 kb on - strand, within PP_0264 at 319.584 kb on + strand at 319.584 kb on + strand at 319.584 kb on + strand at 319.584 kb on + strand at 319.585 kb on - strand at 319.585 kb on - strand at 319.585 kb on - strand at 319.585 kb on - strand at 319.629 kb on + strand at 319.629 kb on + strand at 319.629 kb on + strand at 319.632 kb on + strand at 319.632 kb on + strand at 319.632 kb on + strand at 319.633 kb on - strand at 319.633 kb on - strand at 319.662 kb on - strand at 319.662 kb on - strand at 319.662 kb on - strand at 319.664 kb on + strand at 319.664 kb on + strand at 319.664 kb on + strand at 319.664 kb on + strand at 319.665 kb on - strand at 319.774 kb on + strand at 319.775 kb on - strand at 319.775 kb on - strand at 319.775 kb on - strand at 319.781 kb on + strand at 319.781 kb on + strand at 319.782 kb on - strand at 319.782 kb on - strand at 319.786 kb on - strand at 319.786 kb on - strand at 319.897 kb on + strand, within PP_0265 at 319.898 kb on - strand, within PP_0265 at 319.920 kb on + strand, within PP_0265 at 319.920 kb on + strand, within PP_0265 at 319.920 kb on + strand, within PP_0265 at 319.923 kb on + strand, within PP_0265 at 319.924 kb on - strand, within PP_0265 at 319.924 kb on - strand, within PP_0265 at 319.938 kb on + strand, within PP_0265 at 320.143 kb on - strand, within PP_0265 at 320.297 kb on + strand at 320.298 kb on - strand at 320.298 kb on - strand at 320.298 kb on - strand at 320.298 kb on - strand at 320.314 kb on + strand at 320.356 kb on + strand at 320.357 kb on - strand at 320.357 kb on - strand at 320.357 kb on - strand at 320.357 kb on - strand at 320.366 kb on - strand at 320.366 kb on - strand at 320.395 kb on - strand at 320.412 kb on + strand at 320.412 kb on + strand at 320.412 kb on + strand at 320.412 kb on + strand at 320.413 kb on - strand at 320.413 kb on - strand at 320.413 kb on - strand at 320.413 kb on - strand at 320.413 kb on - strand at 320.423 kb on + strand at 320.423 kb on + strand at 320.424 kb on - strand at 320.424 kb on - strand at 320.428 kb on + strand at 320.681 kb on - strand at 320.681 kb on - strand at 320.681 kb on - strand at 320.749 kb on + strand, within PP_0266 at 320.900 kb on + strand, within PP_0266 at 320.900 kb on + strand, within PP_0266 at 320.938 kb on + strand, within PP_0266 at 320.938 kb on + strand, within PP_0266 at 320.938 kb on + strand, within PP_0266 at 320.939 kb on - strand, within PP_0266 at 320.982 kb on + strand, within PP_0266 at 320.982 kb on + strand, within PP_0266 at 320.982 kb on + strand, within PP_0266 at 321.091 kb on + strand, within PP_0266 at 321.091 kb on + strand, within PP_0266 at 321.092 kb on - strand at 321.214 kb on + strand, within PP_0266 at 321.214 kb on + strand, within PP_0266 at 321.215 kb on - strand, within PP_0266 at 321.234 kb on + strand, within PP_0266 at 321.234 kb on + strand, within PP_0266 at 321.235 kb on - strand, within PP_0266 at 321.276 kb on + strand, within PP_0266 at 321.276 kb on + strand, within PP_0266 at 321.299 kb on - strand, within PP_0266 at 321.299 kb on - strand, within PP_0266 at 321.299 kb on - strand, within PP_0266
Per-strain Table
Position Strand Gene LocusTag Fraction PUT 8 MOI 1 remove 318,816 + PP_0264 0.49 +2.7 318,816 + PP_0264 0.49 -1.0 318,816 + PP_0264 0.49 +0.4 318,817 - PP_0264 0.49 +2.0 318,817 - PP_0264 0.49 +0.4 318,860 + PP_0264 0.51 -0.5 318,861 - PP_0264 0.51 +0.3 318,890 + PP_0264 0.53 -1.0 319,191 - PP_0264 0.70 -4.0 319,324 - PP_0264 0.77 +1.2 319,324 - PP_0264 0.77 +0.8 319,413 + PP_0264 0.82 -1.9 319,413 + PP_0264 0.82 +0.0 319,414 - PP_0264 0.82 +0.5 319,414 - PP_0264 0.82 +0.9 319,414 - PP_0264 0.82 -0.6 319,584 + -3.8 319,584 + -4.0 319,584 + -0.7 319,584 + +1.8 319,585 - +0.2 319,585 - -0.2 319,585 - +0.2 319,585 - +0.8 319,629 + -3.9 319,629 + -0.2 319,629 + +0.9 319,632 + +1.5 319,632 + -0.4 319,632 + -3.8 319,633 - -0.5 319,633 - -2.5 319,662 - -4.2 319,662 - -0.3 319,662 - -0.5 319,664 + +0.3 319,664 + +1.0 319,664 + +0.1 319,664 + +0.2 319,665 - +0.8 319,774 + -3.8 319,775 - +0.7 319,775 - +1.1 319,775 - +1.2 319,781 + +0.2 319,781 + -0.2 319,782 - -2.4 319,782 - -0.4 319,786 - -0.2 319,786 - +0.2 319,897 + PP_0265 0.21 +1.3 319,898 - PP_0265 0.21 +0.4 319,920 + PP_0265 0.25 +0.8 319,920 + PP_0265 0.25 +1.5 319,920 + PP_0265 0.25 +0.4 319,923 + PP_0265 0.25 -0.9 319,924 - PP_0265 0.26 -0.1 319,924 - PP_0265 0.26 +1.1 319,938 + PP_0265 0.28 -1.0 320,143 - PP_0265 0.66 -0.3 320,297 + +1.2 320,298 - -1.6 320,298 - +2.0 320,298 - -2.3 320,298 - -0.7 320,314 + +1.7 320,356 + +2.0 320,357 - +1.3 320,357 - +0.1 320,357 - -0.6 320,357 - -0.1 320,366 - -2.5 320,366 - +0.2 320,395 - -4.1 320,412 + -3.9 320,412 + +2.0 320,412 + +0.0 320,412 + -0.6 320,413 - -0.5 320,413 - -0.1 320,413 - +1.1 320,413 - -2.2 320,413 - -1.4 320,423 + +0.2 320,423 + -0.5 320,424 - -0.4 320,424 - -0.4 320,428 + +0.4 320,681 - +0.5 320,681 - -1.7 320,681 - +3.0 320,749 + PP_0266 0.13 +0.5 320,900 + PP_0266 0.26 +1.4 320,900 + PP_0266 0.26 -0.1 320,938 + PP_0266 0.30 -0.7 320,938 + PP_0266 0.30 +0.5 320,938 + PP_0266 0.30 +0.3 320,939 - PP_0266 0.30 +2.3 320,982 + PP_0266 0.34 +0.7 320,982 + PP_0266 0.34 +0.8 320,982 + PP_0266 0.34 -0.7 321,091 + PP_0266 0.44 -0.5 321,091 + PP_0266 0.44 +1.2 321,092 - +2.7 321,214 + PP_0266 0.55 +1.2 321,214 + PP_0266 0.55 +0.1 321,215 - PP_0266 0.55 +0.3 321,234 + PP_0266 0.57 +0.7 321,234 + PP_0266 0.57 -0.6 321,235 - PP_0266 0.57 +3.3 321,276 + PP_0266 0.60 +0.0 321,276 + PP_0266 0.60 -1.6 321,299 - PP_0266 0.63 -0.6 321,299 - PP_0266 0.63 +4.4 321,299 - PP_0266 0.63 +5.3
Or see this region's nucleotide sequence