Experiment: PUT 8 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0218 and PP_0219 are separated by 28 nucleotides PP_0219 and PP_0220 overlap by 11 nucleotides
PP_0218: PP_0218 - Sensory box protein, at 270,100 to 271,995
_0218
PP_0219: PP_0219 - L,D-methionine D-methionine ABC transporter - permease subunit, at 272,024 to 272,668
_0219
PP_0220: PP_0220 - methionine import ATP-binding protein metN2, at 272,658 to 273,767
_0220
Position (kb) 272
273 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 271.047 kb on + strand, within PP_0218 at 271.047 kb on + strand, within PP_0218 at 271.048 kb on - strand, within PP_0218 at 271.171 kb on + strand, within PP_0218 at 271.171 kb on + strand, within PP_0218 at 271.171 kb on + strand, within PP_0218 at 271.171 kb on + strand, within PP_0218 at 271.172 kb on - strand, within PP_0218 at 271.172 kb on - strand, within PP_0218 at 271.201 kb on + strand, within PP_0218 at 271.201 kb on + strand, within PP_0218 at 271.201 kb on + strand, within PP_0218 at 271.201 kb on + strand, within PP_0218 at 271.202 kb on - strand, within PP_0218 at 271.202 kb on - strand, within PP_0218 at 271.211 kb on - strand, within PP_0218 at 271.211 kb on - strand, within PP_0218 at 271.300 kb on + strand, within PP_0218 at 271.300 kb on + strand, within PP_0218 at 271.446 kb on + strand, within PP_0218 at 271.562 kb on - strand, within PP_0218 at 271.857 kb on + strand at 271.948 kb on + strand at 272.082 kb on - strand at 272.136 kb on + strand, within PP_0219 at 272.221 kb on + strand, within PP_0219 at 272.221 kb on + strand, within PP_0219 at 272.221 kb on + strand, within PP_0219 at 272.267 kb on + strand, within PP_0219 at 272.267 kb on + strand, within PP_0219 at 272.267 kb on + strand, within PP_0219 at 272.267 kb on + strand, within PP_0219 at 272.267 kb on + strand, within PP_0219 at 272.267 kb on + strand, within PP_0219 at 272.267 kb on + strand, within PP_0219 at 272.267 kb on + strand, within PP_0219 at 272.268 kb on - strand, within PP_0219 at 272.268 kb on - strand, within PP_0219 at 272.268 kb on - strand, within PP_0219 at 272.418 kb on + strand, within PP_0219 at 272.418 kb on + strand, within PP_0219 at 272.418 kb on + strand, within PP_0219 at 272.418 kb on + strand, within PP_0219 at 272.418 kb on + strand, within PP_0219 at 272.418 kb on + strand, within PP_0219 at 272.419 kb on - strand, within PP_0219 at 272.419 kb on - strand, within PP_0219 at 272.419 kb on - strand, within PP_0219 at 272.419 kb on - strand, within PP_0219 at 272.582 kb on + strand, within PP_0219 at 272.582 kb on + strand, within PP_0219 at 272.583 kb on - strand, within PP_0219 at 272.632 kb on + strand at 272.633 kb on - strand at 272.633 kb on - strand at 272.676 kb on + strand at 272.677 kb on - strand at 273.271 kb on + strand, within PP_0220 at 273.495 kb on + strand, within PP_0220 at 273.551 kb on + strand, within PP_0220 at 273.552 kb on - strand at 273.552 kb on - strand, within PP_0220 at 273.644 kb on - strand, within PP_0220
Per-strain Table
Position Strand Gene LocusTag Fraction PUT 8 MOI 1 remove 271,047 + PP_0218 0.50 -0.9 271,047 + PP_0218 0.50 -3.9 271,048 - PP_0218 0.50 -3.6 271,171 + PP_0218 0.56 +0.2 271,171 + PP_0218 0.56 -1.7 271,171 + PP_0218 0.56 +0.5 271,171 + PP_0218 0.56 +1.1 271,172 - PP_0218 0.57 -0.6 271,172 - PP_0218 0.57 -1.1 271,201 + PP_0218 0.58 -3.8 271,201 + PP_0218 0.58 +0.7 271,201 + PP_0218 0.58 -4.3 271,201 + PP_0218 0.58 -3.4 271,202 - PP_0218 0.58 -2.0 271,202 - PP_0218 0.58 -4.3 271,211 - PP_0218 0.59 -3.9 271,211 - PP_0218 0.59 -0.6 271,300 + PP_0218 0.63 -2.5 271,300 + PP_0218 0.63 +1.4 271,446 + PP_0218 0.71 -2.3 271,562 - PP_0218 0.77 -4.5 271,857 + -3.6 271,948 + -3.2 272,082 - -3.1 272,136 + PP_0219 0.17 +0.7 272,221 + PP_0219 0.31 -0.8 272,221 + PP_0219 0.31 +0.5 272,221 + PP_0219 0.31 +1.3 272,267 + PP_0219 0.38 +0.7 272,267 + PP_0219 0.38 +1.1 272,267 + PP_0219 0.38 +0.8 272,267 + PP_0219 0.38 +1.4 272,267 + PP_0219 0.38 -3.9 272,267 + PP_0219 0.38 -3.6 272,267 + PP_0219 0.38 +1.7 272,267 + PP_0219 0.38 +1.3 272,268 - PP_0219 0.38 +0.6 272,268 - PP_0219 0.38 +0.5 272,268 - PP_0219 0.38 -3.8 272,418 + PP_0219 0.61 +0.6 272,418 + PP_0219 0.61 -1.0 272,418 + PP_0219 0.61 -4.2 272,418 + PP_0219 0.61 -0.7 272,418 + PP_0219 0.61 +1.6 272,418 + PP_0219 0.61 +1.4 272,419 - PP_0219 0.61 -4.9 272,419 - PP_0219 0.61 +1.6 272,419 - PP_0219 0.61 +1.8 272,419 - PP_0219 0.61 -4.3 272,582 + PP_0219 0.87 +1.2 272,582 + PP_0219 0.87 +0.6 272,583 - PP_0219 0.87 -1.2 272,632 + +1.0 272,633 - +0.1 272,633 - +0.5 272,676 + -0.8 272,677 - +0.9 273,271 + PP_0220 0.55 +0.7 273,495 + PP_0220 0.75 +1.0 273,551 + PP_0220 0.80 -0.3 273,552 - -0.9 273,552 - PP_0220 0.81 +0.7 273,644 - PP_0220 0.89 +1.4
Or see this region's nucleotide sequence