Experiment: PUT 8 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0065 and PP_0066 are separated by 23 nucleotides PP_0066 and PP_0067 overlap by 4 nucleotides PP_0067 and PP_0068 are separated by 60 nucleotides
PP_0065: PP_0065 - NAD-binding component of TrK potassium transporter, at 74,836 to 76,209
_0065
PP_0066: PP_0066 - Ribosomal RNA small subunit methyltransferase B, at 76,233 to 77,543
_0066
PP_0067: PP_0067 - Methionyl-tRNA formyltransferase, at 77,540 to 78,472
_0067
PP_0068: PP_0068 - peptide deformylase, at 78,533 to 79,039
_0068
Position (kb) 76
77
78 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3 at 75.431 kb on + strand, within PP_0065 at 75.517 kb on - strand, within PP_0065 at 75.728 kb on + strand, within PP_0065 at 75.729 kb on - strand, within PP_0065 at 75.729 kb on - strand, within PP_0065 at 75.732 kb on - strand at 75.818 kb on + strand, within PP_0065 at 75.818 kb on + strand, within PP_0065 at 75.818 kb on + strand, within PP_0065 at 75.818 kb on + strand, within PP_0065 at 75.819 kb on - strand, within PP_0065 at 76.131 kb on + strand at 76.131 kb on + strand at 76.222 kb on - strand at 76.234 kb on + strand at 76.235 kb on - strand at 76.270 kb on + strand at 76.271 kb on - strand at 76.271 kb on - strand at 76.273 kb on + strand at 76.273 kb on + strand at 76.273 kb on + strand at 76.273 kb on + strand at 76.274 kb on - strand at 76.274 kb on - strand at 76.435 kb on + strand, within PP_0066 at 76.435 kb on + strand, within PP_0066 at 76.435 kb on + strand, within PP_0066 at 76.436 kb on - strand, within PP_0066 at 76.436 kb on - strand, within PP_0066 at 76.436 kb on - strand, within PP_0066 at 76.436 kb on - strand, within PP_0066 at 76.437 kb on + strand, within PP_0066 at 76.437 kb on + strand, within PP_0066 at 76.437 kb on + strand, within PP_0066 at 76.438 kb on - strand, within PP_0066 at 76.438 kb on - strand, within PP_0066 at 76.438 kb on - strand, within PP_0066 at 76.942 kb on + strand, within PP_0066 at 76.942 kb on + strand, within PP_0066 at 76.990 kb on + strand, within PP_0066 at 76.991 kb on - strand, within PP_0066 at 76.991 kb on - strand, within PP_0066 at 76.991 kb on - strand, within PP_0066 at 77.299 kb on + strand, within PP_0066 at 77.448 kb on + strand at 77.448 kb on + strand at 77.449 kb on - strand at 77.449 kb on - strand at 77.449 kb on - strand at 77.449 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction PUT 8 MOI 1 remove 75,431 + PP_0065 0.43 +2.6 75,517 - PP_0065 0.50 +1.9 75,728 + PP_0065 0.65 -2.1 75,729 - PP_0065 0.65 +2.8 75,729 - PP_0065 0.65 -2.3 75,732 - +0.3 75,818 + PP_0065 0.71 +1.8 75,818 + PP_0065 0.71 +3.2 75,818 + PP_0065 0.71 +1.3 75,818 + PP_0065 0.71 +1.2 75,819 - PP_0065 0.72 +3.0 76,131 + +2.6 76,131 + -0.8 76,222 - -2.8 76,234 + -2.1 76,235 - +1.0 76,270 + -0.7 76,271 - -4.7 76,271 - -5.3 76,273 + -4.4 76,273 + -3.9 76,273 + -4.4 76,273 + -5.1 76,274 - -4.9 76,274 - -4.9 76,435 + PP_0066 0.15 -4.2 76,435 + PP_0066 0.15 -1.7 76,435 + PP_0066 0.15 -3.6 76,436 - PP_0066 0.15 -4.9 76,436 - PP_0066 0.15 -4.6 76,436 - PP_0066 0.15 -5.2 76,436 - PP_0066 0.15 -3.6 76,437 + PP_0066 0.16 -4.5 76,437 + PP_0066 0.16 -3.1 76,437 + PP_0066 0.16 -3.0 76,438 - PP_0066 0.16 -4.4 76,438 - PP_0066 0.16 -4.8 76,438 - PP_0066 0.16 -4.4 76,942 + PP_0066 0.54 -2.8 76,942 + PP_0066 0.54 -3.7 76,990 + PP_0066 0.58 -1.3 76,991 - PP_0066 0.58 -2.9 76,991 - PP_0066 0.58 -3.6 76,991 - PP_0066 0.58 -1.2 77,299 + PP_0066 0.81 -2.5 77,448 + -2.5 77,448 + -4.2 77,449 - -4.6 77,449 - -4.0 77,449 - -4.5 77,449 - -3.5
Or see this region's nucleotide sequence