Strain Fitness in Pseudomonas putida KT2440 around PP_0063

Experiment: PUT 8 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPP_0061 and PP_0062 are separated by 83 nucleotidesPP_0062 and PP_0063 are separated by 41 nucleotidesPP_0063 and PP_0064 are separated by 97 nucleotidesPP_0064 and PP_0065 are separated by 11 nucleotides PP_0061: PP_0061 - glycine-tRNA ligase alpha subunit, at 71,901 to 72,848 _0061 PP_0062: PP_0062 - 3-methyl-adenine DNA glycosylase I, at 72,932 to 73,483 _0062 PP_0063: PP_0063 - putative Lipid A biosynthesis lauroyl acyltransferase, at 73,525 to 74,412 _0063 PP_0064: PP_0064 - conserved protein of unknown function, at 74,510 to 74,824 _0064 PP_0065: PP_0065 - NAD-binding component of TrK potassium transporter, at 74,836 to 76,209 _0065 Position (kb) 73 74 75Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3 4at 72.893 kb on - strandat 72.893 kb on - strandat 72.893 kb on - strandat 72.895 kb on - strandat 72.917 kb on - strandat 72.932 kb on - strandat 72.932 kb on - strandat 72.969 kb on - strandat 73.073 kb on - strand, within PP_0062at 73.073 kb on - strand, within PP_0062at 73.112 kb on + strand, within PP_0062at 73.112 kb on + strand, within PP_0062at 73.113 kb on - strand, within PP_0062at 73.113 kb on - strand, within PP_0062at 73.113 kb on - strand, within PP_0062at 73.113 kb on - strand, within PP_0062at 73.128 kb on + strand, within PP_0062at 73.406 kb on + strand, within PP_0062at 73.406 kb on + strand, within PP_0062at 73.407 kb on - strand, within PP_0062at 73.489 kb on + strandat 73.489 kb on + strandat 73.490 kb on - strandat 73.490 kb on - strandat 73.490 kb on - strandat 73.512 kb on - strandat 73.662 kb on - strand, within PP_0063at 73.746 kb on + strandat 73.747 kb on - strand, within PP_0063at 73.858 kb on - strand, within PP_0063at 73.915 kb on + strand, within PP_0063at 73.915 kb on + strand, within PP_0063at 73.915 kb on + strand, within PP_0063at 73.915 kb on + strand, within PP_0063at 73.916 kb on - strand, within PP_0063at 74.082 kb on + strand, within PP_0063at 74.195 kb on + strand, within PP_0063at 74.196 kb on - strand, within PP_0063at 74.275 kb on + strand, within PP_0063at 74.275 kb on + strand, within PP_0063at 74.275 kb on + strand, within PP_0063at 74.276 kb on - strand, within PP_0063at 74.276 kb on - strand, within PP_0063at 74.276 kb on - strand, within PP_0063at 74.276 kb on - strand, within PP_0063at 74.276 kb on - strand, within PP_0063at 74.329 kb on + strandat 74.330 kb on - strandat 74.330 kb on - strandat 74.330 kb on - strandat 74.330 kb on - strandat 74.341 kb on + strandat 74.353 kb on + strandat 74.353 kb on + strandat 74.354 kb on - strandat 74.354 kb on - strandat 74.354 kb on - strandat 74.410 kb on + strandat 74.410 kb on + strandat 74.411 kb on - strandat 74.411 kb on - strandat 74.411 kb on - strandat 74.411 kb on - strandat 74.411 kb on - strandat 74.411 kb on - strandat 74.431 kb on + strandat 74.431 kb on + strandat 74.431 kb on + strandat 74.431 kb on + strandat 74.431 kb on + strandat 74.497 kb on + strandat 74.497 kb on + strandat 74.498 kb on - strandat 74.646 kb on + strand, within PP_0064at 74.647 kb on - strand, within PP_0064at 74.647 kb on - strand, within PP_0064at 74.647 kb on - strand, within PP_0064at 74.654 kb on - strand, within PP_0064at 74.679 kb on + strand, within PP_0064at 74.680 kb on - strand, within PP_0064at 74.696 kb on + strand, within PP_0064at 74.985 kb on + strand, within PP_0065at 75.196 kb on + strand, within PP_0065at 75.196 kb on + strand, within PP_0065at 75.209 kb on + strand, within PP_0065

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Per-strain Table

Position Strand Gene LocusTag Fraction PUT 8 MOI 1
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72,893 - +1.6
72,893 - -3.9
72,893 - +1.3
72,895 - -1.0
72,917 - +0.3
72,932 - +1.0
72,932 - -0.4
72,969 - -0.4
73,073 - PP_0062 0.26 -4.6
73,073 - PP_0062 0.26 -0.8
73,112 + PP_0062 0.33 +0.6
73,112 + PP_0062 0.33 +0.4
73,113 - PP_0062 0.33 -2.3
73,113 - PP_0062 0.33 -0.8
73,113 - PP_0062 0.33 +0.6
73,113 - PP_0062 0.33 +2.9
73,128 + PP_0062 0.36 +0.3
73,406 + PP_0062 0.86 +0.2
73,406 + PP_0062 0.86 -1.2
73,407 - PP_0062 0.86 +0.8
73,489 + -0.2
73,489 + +1.7
73,490 - +2.0
73,490 - +1.2
73,490 - +1.9
73,512 - -0.1
73,662 - PP_0063 0.15 +1.7
73,746 + -0.1
73,747 - PP_0063 0.25 +4.4
73,858 - PP_0063 0.38 -1.7
73,915 + PP_0063 0.44 +2.9
73,915 + PP_0063 0.44 -1.2
73,915 + PP_0063 0.44 +2.3
73,915 + PP_0063 0.44 +1.9
73,916 - PP_0063 0.44 +1.9
74,082 + PP_0063 0.63 +3.0
74,195 + PP_0063 0.75 +3.2
74,196 - PP_0063 0.76 +3.0
74,275 + PP_0063 0.84 -0.9
74,275 + PP_0063 0.84 -0.3
74,275 + PP_0063 0.84 +2.3
74,276 - PP_0063 0.85 -0.5
74,276 - PP_0063 0.85 +2.3
74,276 - PP_0063 0.85 +0.3
74,276 - PP_0063 0.85 +3.9
74,276 - PP_0063 0.85 -2.0
74,329 + -1.2
74,330 - -1.6
74,330 - +0.7
74,330 - -0.0
74,330 - -0.7
74,341 + +3.3
74,353 + -0.5
74,353 + -1.7
74,354 - -0.3
74,354 - +2.3
74,354 - +1.9
74,410 + +1.0
74,410 + -0.3
74,411 - -0.0
74,411 - +0.8
74,411 - +2.1
74,411 - -0.3
74,411 - -0.8
74,411 - +0.8
74,431 + +0.4
74,431 + +0.5
74,431 + +0.5
74,431 + -4.1
74,431 + -0.9
74,497 + -0.0
74,497 + -3.3
74,498 - +0.3
74,646 + PP_0064 0.43 +0.4
74,647 - PP_0064 0.43 +0.6
74,647 - PP_0064 0.43 +0.6
74,647 - PP_0064 0.43 +2.4
74,654 - PP_0064 0.46 +0.5
74,679 + PP_0064 0.54 -1.1
74,680 - PP_0064 0.54 -2.6
74,696 + PP_0064 0.59 -2.7
74,985 + PP_0065 0.11 +1.3
75,196 + PP_0065 0.26 -0.5
75,196 + PP_0065 0.26 -3.1
75,209 + PP_0065 0.27 -2.1

Or see this region's nucleotide sequence