Experiment: PUT8 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_2455 and PP_2456 overlap by 4 nucleotides PP_2456 and PP_2457 are separated by 4 nucleotides
PP_2455: PP_2455 - ribose ABC transporter - ATP-binding subunit, at 2,801,617 to 2,803,191
_2455
PP_2456: PP_2456 - D-ribose ABC transporter - permease subunit, at 2,803,188 to 2,804,183
_2456
PP_2457: PP_2457 - DNA-binding transcriptional repressor, at 2,804,188 to 2,805,210
_2457
Position (kb)
2803
2804
2805 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2802.235 kb on + strand, within PP_2455 at 2802.236 kb on - strand, within PP_2455 at 2802.236 kb on - strand, within PP_2455 at 2802.236 kb on - strand, within PP_2455 at 2802.237 kb on + strand, within PP_2455 at 2802.238 kb on - strand, within PP_2455 at 2802.332 kb on - strand, within PP_2455 at 2802.332 kb on - strand at 2802.384 kb on + strand, within PP_2455 at 2802.384 kb on + strand, within PP_2455 at 2802.385 kb on - strand, within PP_2455 at 2802.547 kb on + strand, within PP_2455 at 2802.548 kb on - strand, within PP_2455 at 2802.548 kb on - strand, within PP_2455 at 2802.958 kb on + strand, within PP_2455 at 2802.958 kb on + strand, within PP_2455 at 2802.959 kb on - strand, within PP_2455 at 2802.959 kb on - strand, within PP_2455 at 2803.246 kb on - strand at 2803.263 kb on + strand at 2803.264 kb on - strand at 2803.264 kb on - strand at 2803.335 kb on + strand, within PP_2456 at 2803.336 kb on - strand, within PP_2456 at 2803.636 kb on - strand, within PP_2456 at 2803.662 kb on + strand, within PP_2456 at 2803.662 kb on + strand, within PP_2456 at 2803.662 kb on + strand, within PP_2456 at 2803.663 kb on - strand, within PP_2456 at 2803.663 kb on - strand, within PP_2456 at 2803.762 kb on + strand, within PP_2456 at 2803.851 kb on + strand, within PP_2456 at 2803.852 kb on - strand, within PP_2456 at 2804.152 kb on - strand at 2804.156 kb on + strand at 2804.230 kb on + strand at 2804.230 kb on + strand at 2804.249 kb on + strand at 2804.249 kb on + strand at 2804.249 kb on + strand at 2804.250 kb on - strand at 2804.250 kb on - strand at 2804.250 kb on - strand at 2804.250 kb on - strand at 2804.456 kb on - strand, within PP_2457 at 2804.507 kb on - strand, within PP_2457 at 2804.546 kb on + strand, within PP_2457 at 2804.680 kb on + strand, within PP_2457 at 2804.681 kb on - strand, within PP_2457 at 2804.681 kb on - strand, within PP_2457 at 2804.728 kb on + strand, within PP_2457 at 2804.729 kb on - strand, within PP_2457 at 2804.730 kb on + strand, within PP_2457 at 2804.730 kb on - strand, within PP_2457 at 2804.828 kb on + strand, within PP_2457 at 2804.828 kb on + strand, within PP_2457 at 2804.829 kb on - strand at 2804.972 kb on + strand, within PP_2457 at 2805.022 kb on + strand, within PP_2457 at 2805.023 kb on - strand, within PP_2457 at 2805.023 kb on - strand, within PP_2457
Per-strain Table
Position Strand Gene LocusTag Fraction PUT8 MOI 10 remove 2,802,235 + PP_2455 0.39 +0.6 2,802,236 - PP_2455 0.39 +0.4 2,802,236 - PP_2455 0.39 -2.0 2,802,236 - PP_2455 0.39 -0.7 2,802,237 + PP_2455 0.39 -0.1 2,802,238 - PP_2455 0.39 +0.0 2,802,332 - PP_2455 0.45 +1.0 2,802,332 - +0.8 2,802,384 + PP_2455 0.49 +0.8 2,802,384 + PP_2455 0.49 +1.8 2,802,385 - PP_2455 0.49 +0.0 2,802,547 + PP_2455 0.59 -1.0 2,802,548 - PP_2455 0.59 +0.2 2,802,548 - PP_2455 0.59 +0.4 2,802,958 + PP_2455 0.85 +0.8 2,802,958 + PP_2455 0.85 +0.8 2,802,959 - PP_2455 0.85 +3.2 2,802,959 - PP_2455 0.85 +0.3 2,803,246 - -0.3 2,803,263 + +2.1 2,803,264 - +0.5 2,803,264 - +0.6 2,803,335 + PP_2456 0.15 +0.4 2,803,336 - PP_2456 0.15 +0.5 2,803,636 - PP_2456 0.45 +0.9 2,803,662 + PP_2456 0.48 -1.0 2,803,662 + PP_2456 0.48 +0.7 2,803,662 + PP_2456 0.48 +1.6 2,803,663 - PP_2456 0.48 +0.2 2,803,663 - PP_2456 0.48 +1.3 2,803,762 + PP_2456 0.58 +0.8 2,803,851 + PP_2456 0.67 +1.8 2,803,852 - PP_2456 0.67 +1.8 2,804,152 - +0.5 2,804,156 + -0.3 2,804,230 + -0.5 2,804,230 + +1.9 2,804,249 + -0.7 2,804,249 + -0.3 2,804,249 + +2.0 2,804,250 - +0.6 2,804,250 - +0.2 2,804,250 - -0.7 2,804,250 - -0.6 2,804,456 - PP_2457 0.26 +0.1 2,804,507 - PP_2457 0.31 +0.2 2,804,546 + PP_2457 0.35 -0.2 2,804,680 + PP_2457 0.48 +1.0 2,804,681 - PP_2457 0.48 -1.4 2,804,681 - PP_2457 0.48 -3.1 2,804,728 + PP_2457 0.53 -1.8 2,804,729 - PP_2457 0.53 +0.4 2,804,730 + PP_2457 0.53 -0.9 2,804,730 - PP_2457 0.53 +0.4 2,804,828 + PP_2457 0.63 +0.6 2,804,828 + PP_2457 0.63 +2.6 2,804,829 - +1.0 2,804,972 + PP_2457 0.77 +0.1 2,805,022 + PP_2457 0.82 +0.6 2,805,023 - PP_2457 0.82 +0.0 2,805,023 - PP_2457 0.82 -0.9
Or see this region's nucleotide sequence