Experiment: Utah_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ribB and b3042 are separated by 373 nucleotides b3042 and ygiL are separated by 283 nucleotides ygiL and yqiG are separated by 95 nucleotides yqiG and insC are separated by 97 nucleotides insC and insD-5 overlap by 43 nucleotides
b3041: ribB - 3,4-dihydroxy-2-butanone 4-phosphate synthase (NCBI), at 3,181,835 to 3,182,488
ribB
b3042: b3042 - orf, hypothetical protein (VIMSS), at 3,182,862 to 3,183,152
b3042
b3043: ygiL - predicted fimbrial-like adhesin protein (NCBI), at 3,183,436 to 3,183,987
ygiL
b3046: yqiG - predicted outer membrane usher protein (RefSeq), at 3,184,083 to 3,184,111
yqiG
b3044: insC - KpLE2 phage-like element; IS2 insertion element repressor InsA (RefSeq), at 3,184,209 to 3,184,574
insC
b3045: insD-5 - IS2 insertion element transposase InsAB' (NCBI), at 3,184,532 to 3,185,437
insD-5
Position (kb)
3183
3184 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3182.734 kb on - strand at 3182.734 kb on - strand at 3182.755 kb on - strand at 3182.776 kb on + strand at 3182.776 kb on + strand at 3182.804 kb on + strand at 3182.804 kb on + strand at 3182.834 kb on + strand at 3182.839 kb on - strand at 3182.839 kb on - strand at 3182.846 kb on + strand at 3182.846 kb on + strand at 3182.847 kb on - strand at 3182.968 kb on + strand, within b3042 at 3182.968 kb on + strand, within b3042 at 3182.981 kb on + strand, within b3042 at 3183.100 kb on + strand, within b3042 at 3183.123 kb on + strand, within b3042 at 3183.123 kb on + strand, within b3042 at 3183.124 kb on + strand at 3183.131 kb on - strand at 3183.133 kb on + strand at 3183.137 kb on + strand at 3183.137 kb on + strand at 3183.137 kb on + strand at 3183.144 kb on + strand at 3183.146 kb on + strand at 3183.210 kb on + strand at 3183.260 kb on + strand at 3183.309 kb on + strand at 3183.482 kb on - strand at 3183.595 kb on - strand, within ygiL at 3183.612 kb on - strand, within ygiL at 3183.612 kb on - strand, within ygiL at 3183.614 kb on + strand, within ygiL at 3183.648 kb on + strand, within ygiL at 3183.648 kb on + strand, within ygiL at 3183.648 kb on + strand, within ygiL at 3183.666 kb on + strand, within ygiL at 3183.666 kb on + strand, within ygiL at 3183.677 kb on - strand, within ygiL at 3183.677 kb on - strand, within ygiL at 3183.682 kb on + strand, within ygiL at 3183.682 kb on + strand, within ygiL at 3183.687 kb on + strand, within ygiL at 3183.689 kb on + strand, within ygiL at 3183.689 kb on + strand, within ygiL at 3183.689 kb on - strand, within ygiL at 3183.735 kb on + strand, within ygiL at 3183.750 kb on + strand, within ygiL at 3183.802 kb on - strand, within ygiL at 3183.805 kb on + strand, within ygiL at 3183.809 kb on + strand, within ygiL at 3183.809 kb on + strand, within ygiL at 3183.813 kb on - strand, within ygiL at 3183.865 kb on + strand, within ygiL at 3183.892 kb on - strand, within ygiL at 3183.937 kb on + strand at 3183.948 kb on + strand at 3183.948 kb on + strand at 3183.950 kb on + strand at 3183.950 kb on + strand at 3183.960 kb on + strand at 3183.964 kb on + strand at 3183.964 kb on + strand at 3183.964 kb on + strand at 3183.964 kb on + strand at 3183.964 kb on + strand at 3183.979 kb on - strand at 3183.986 kb on + strand at 3184.001 kb on + strand at 3184.013 kb on - strand at 3184.015 kb on + strand at 3184.016 kb on + strand at 3184.016 kb on + strand at 3184.034 kb on - strand at 3184.067 kb on + strand at 3184.067 kb on + strand at 3184.122 kb on - strand at 3184.122 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Utah_phage 15 MOI remove 3,182,734 - +0.1 3,182,734 - -0.5 3,182,755 - +1.6 3,182,776 + +0.0 3,182,776 + +0.3 3,182,804 + +0.6 3,182,804 + +1.2 3,182,834 + +0.0 3,182,839 - -1.6 3,182,839 - -1.3 3,182,846 + +0.1 3,182,846 + +0.5 3,182,847 - +1.5 3,182,968 + b3042 b3042 0.36 +0.2 3,182,968 + b3042 b3042 0.36 +0.9 3,182,981 + b3042 b3042 0.41 +1.5 3,183,100 + b3042 b3042 0.82 -0.2 3,183,123 + b3042 b3042 0.90 -0.3 3,183,123 + b3042 b3042 0.90 +0.0 3,183,124 + -0.7 3,183,131 - -0.1 3,183,133 + -0.1 3,183,137 + -1.3 3,183,137 + -0.3 3,183,137 + +0.2 3,183,144 + +0.2 3,183,146 + +0.8 3,183,210 + +0.1 3,183,260 + +0.3 3,183,309 + +0.9 3,183,482 - +1.9 3,183,595 - ygiL b3043 0.29 +1.9 3,183,612 - ygiL b3043 0.32 -1.1 3,183,612 - ygiL b3043 0.32 +2.5 3,183,614 + ygiL b3043 0.32 +0.9 3,183,648 + ygiL b3043 0.38 -0.1 3,183,648 + ygiL b3043 0.38 -0.3 3,183,648 + ygiL b3043 0.38 +0.9 3,183,666 + ygiL b3043 0.42 -0.4 3,183,666 + ygiL b3043 0.42 -0.4 3,183,677 - ygiL b3043 0.44 +0.9 3,183,677 - ygiL b3043 0.44 +0.9 3,183,682 + ygiL b3043 0.45 -1.3 3,183,682 + ygiL b3043 0.45 -2.1 3,183,687 + ygiL b3043 0.45 -1.1 3,183,689 + ygiL b3043 0.46 -1.1 3,183,689 + ygiL b3043 0.46 -1.3 3,183,689 - ygiL b3043 0.46 +1.9 3,183,735 + ygiL b3043 0.54 -1.9 3,183,750 + ygiL b3043 0.57 -1.4 3,183,802 - ygiL b3043 0.66 +0.9 3,183,805 + ygiL b3043 0.67 +0.6 3,183,809 + ygiL b3043 0.68 -1.2 3,183,809 + ygiL b3043 0.68 -1.0 3,183,813 - ygiL b3043 0.68 -0.1 3,183,865 + ygiL b3043 0.78 -1.3 3,183,892 - ygiL b3043 0.83 +1.9 3,183,937 + +0.2 3,183,948 + +0.3 3,183,948 + +0.4 3,183,950 + +0.4 3,183,950 + -0.4 3,183,960 + -0.4 3,183,964 + -0.3 3,183,964 + +0.3 3,183,964 + -0.4 3,183,964 + +0.4 3,183,964 + -0.8 3,183,979 - +1.9 3,183,986 + +0.1 3,184,001 + -0.6 3,184,013 - +0.9 3,184,015 + -0.9 3,184,016 + +1.3 3,184,016 + +2.2 3,184,034 - +0.2 3,184,067 + -0.7 3,184,067 + +2.6 3,184,122 - +0.2 3,184,122 - +0.2
Or see this region's nucleotide sequence