Experiment: LB outgrowth control more dilute start
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_5233 and PP_5234 are separated by 46 nucleotides PP_5234 and PP_5723 are separated by 134 nucleotides PP_5723 and PP_5235 are separated by 24 nucleotides PP_5235 and PP_5724 are separated by 0 nucleotides PP_5724 and PP_5236 overlap by 47 nucleotides
PP_5233: PP_5233 - ammonium transporter AmtB, at 5,966,361 to 5,967,692
_5233
PP_5234: PP_5234 - activator of NRII(GlnL/NtrB) phosphatase, at 5,967,739 to 5,968,077
_5234
PP_5723: PP_5723 - protein of unknown function, at 5,968,212 to 5,968,571
_5723
PP_5235: PP_5235 - conserved protein of unknown function, at 5,968,596 to 5,968,862
_5235
PP_5724: PP_5724 - conserved protein of unknown function, at 5,968,863 to 5,969,216
_5724
PP_5236: PP_5236 - conserved protein of unknown function, at 5,969,170 to 5,971,293
_5236
Position (kb)
5968
5969 Strain fitness (log2 ratio)
-1
0
1
2 at 5967.433 kb on + strand, within PP_5233 at 5967.523 kb on + strand, within PP_5233 at 5967.523 kb on + strand, within PP_5233 at 5967.524 kb on - strand, within PP_5233 at 5967.524 kb on - strand, within PP_5233 at 5967.545 kb on + strand, within PP_5233 at 5967.546 kb on - strand, within PP_5233 at 5967.602 kb on + strand at 5967.603 kb on - strand at 5967.645 kb on + strand at 5967.645 kb on + strand at 5967.657 kb on + strand at 5967.658 kb on - strand at 5967.666 kb on + strand at 5967.667 kb on - strand at 5967.667 kb on - strand at 5967.740 kb on + strand at 5967.740 kb on + strand at 5967.740 kb on + strand at 5967.740 kb on + strand at 5967.740 kb on + strand at 5967.741 kb on - strand at 5967.741 kb on - strand at 5967.741 kb on - strand at 5968.135 kb on - strand at 5968.228 kb on - strand at 5968.283 kb on + strand, within PP_5723 at 5968.283 kb on + strand, within PP_5723 at 5968.284 kb on - strand at 5968.284 kb on - strand, within PP_5723 at 5968.470 kb on + strand, within PP_5723 at 5968.471 kb on - strand, within PP_5723 at 5968.471 kb on - strand, within PP_5723 at 5968.557 kb on + strand at 5968.557 kb on + strand at 5968.772 kb on - strand, within PP_5235 at 5968.881 kb on + strand at 5968.889 kb on - strand at 5968.889 kb on - strand at 5968.902 kb on + strand, within PP_5724 at 5968.902 kb on + strand, within PP_5724 at 5968.903 kb on - strand, within PP_5724 at 5968.973 kb on + strand, within PP_5724 at 5968.974 kb on - strand, within PP_5724 at 5968.974 kb on - strand, within PP_5724 at 5968.974 kb on - strand, within PP_5724 at 5969.154 kb on + strand, within PP_5724 at 5969.154 kb on + strand, within PP_5724 at 5969.155 kb on - strand, within PP_5724 at 5969.202 kb on + strand at 5969.202 kb on + strand at 5969.203 kb on - strand at 5969.203 kb on - strand at 5969.278 kb on + strand at 5969.278 kb on + strand at 5969.278 kb on + strand at 5969.279 kb on - strand at 5969.279 kb on - strand at 5969.279 kb on - strand at 5969.279 kb on - strand at 5969.328 kb on + strand at 5969.549 kb on + strand, within PP_5236 at 5969.550 kb on - strand, within PP_5236
Per-strain Table
Position Strand Gene LocusTag Fraction LB outgrowth control more dilute start remove 5,967,433 + PP_5233 0.80 +0.3 5,967,523 + PP_5233 0.87 +0.2 5,967,523 + PP_5233 0.87 -0.4 5,967,524 - PP_5233 0.87 -1.0 5,967,524 - PP_5233 0.87 -0.5 5,967,545 + PP_5233 0.89 +0.6 5,967,546 - PP_5233 0.89 +0.4 5,967,602 + -0.2 5,967,603 - +0.3 5,967,645 + -0.5 5,967,645 + +1.1 5,967,657 + -0.9 5,967,658 - +0.4 5,967,666 + -0.8 5,967,667 - +0.1 5,967,667 - -0.6 5,967,740 + +0.3 5,967,740 + -0.8 5,967,740 + -0.2 5,967,740 + +0.1 5,967,740 + -0.3 5,967,741 - +0.2 5,967,741 - +0.4 5,967,741 - -1.0 5,968,135 - -1.1 5,968,228 - -0.1 5,968,283 + PP_5723 0.20 +0.3 5,968,283 + PP_5723 0.20 +0.5 5,968,284 - +2.4 5,968,284 - PP_5723 0.20 +0.5 5,968,470 + PP_5723 0.72 +0.3 5,968,471 - PP_5723 0.72 +0.9 5,968,471 - PP_5723 0.72 -0.2 5,968,557 + +0.3 5,968,557 + +0.1 5,968,772 - PP_5235 0.66 +0.8 5,968,881 + -0.3 5,968,889 - -0.2 5,968,889 - -0.5 5,968,902 + PP_5724 0.11 +0.0 5,968,902 + PP_5724 0.11 +0.9 5,968,903 - PP_5724 0.11 -1.6 5,968,973 + PP_5724 0.31 +0.4 5,968,974 - PP_5724 0.31 +0.7 5,968,974 - PP_5724 0.31 +0.8 5,968,974 - PP_5724 0.31 -0.5 5,969,154 + PP_5724 0.82 +0.1 5,969,154 + PP_5724 0.82 -1.0 5,969,155 - PP_5724 0.82 +0.3 5,969,202 + -0.0 5,969,202 + -0.6 5,969,203 - +0.8 5,969,203 - -1.4 5,969,278 + -1.4 5,969,278 + -0.4 5,969,278 + -0.1 5,969,279 - +0.5 5,969,279 - +0.8 5,969,279 - -0.2 5,969,279 - -0.9 5,969,328 + +1.1 5,969,549 + PP_5236 0.18 +1.6 5,969,550 - PP_5236 0.18 +0.1
Or see this region's nucleotide sequence