Experiment: LB outgrowth control more dilute start
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_5078 and PP_5079 are separated by 96 nucleotides PP_5079 and PP_5080 are separated by 4 nucleotides PP_5080 and PP_5081 overlap by 53 nucleotides PP_5081 and PP_5082 overlap by 4 nucleotides
PP_5078: PP_5078 - 3-dehydroquinate synthase, at 5,799,878 to 5,800,975
_5078
PP_5079: PP_5079 - Shikimate kinase, at 5,801,072 to 5,801,590
_5079
PP_5080: PP_5080 - type 4 pili biogenesis protein, at 5,801,595 to 5,802,833
_5080
PP_5081: PP_5081 - Type IV pili biogenesis protein, at 5,802,781 to 5,803,797
_5081
PP_5082: PP_5082 - Type IV pili biogenesis protein PilN, at 5,803,794 to 5,804,330
_5082
Position (kb) 5801
5802
5803 Strain fitness (log2 ratio) -2
-1
0
1 at 5800.965 kb on - strand at 5800.978 kb on - strand at 5801.062 kb on + strand at 5801.062 kb on + strand at 5801.674 kb on - strand at 5801.704 kb on + strand at 5801.704 kb on + strand at 5801.705 kb on - strand at 5801.705 kb on - strand at 5801.838 kb on + strand, within PP_5080 at 5801.839 kb on - strand, within PP_5080 at 5801.928 kb on + strand, within PP_5080 at 5801.929 kb on - strand, within PP_5080 at 5801.995 kb on + strand, within PP_5080 at 5802.215 kb on - strand, within PP_5080 at 5802.254 kb on - strand, within PP_5080 at 5802.275 kb on + strand, within PP_5080 at 5802.685 kb on - strand, within PP_5080 at 5802.685 kb on - strand, within PP_5080 at 5802.685 kb on - strand, within PP_5080 at 5802.718 kb on + strand at 5802.719 kb on - strand at 5802.838 kb on + strand at 5802.839 kb on - strand at 5802.839 kb on - strand at 5802.839 kb on - strand at 5802.970 kb on + strand, within PP_5081 at 5802.970 kb on + strand, within PP_5081 at 5802.970 kb on + strand, within PP_5081 at 5802.970 kb on + strand, within PP_5081 at 5802.970 kb on + strand, within PP_5081 at 5802.971 kb on - strand, within PP_5081 at 5802.971 kb on - strand, within PP_5081 at 5802.971 kb on - strand, within PP_5081 at 5802.971 kb on - strand, within PP_5081 at 5802.971 kb on - strand, within PP_5081 at 5802.971 kb on - strand, within PP_5081 at 5802.971 kb on - strand, within PP_5081 at 5802.971 kb on - strand, within PP_5081 at 5802.971 kb on - strand, within PP_5081 at 5802.971 kb on - strand, within PP_5081 at 5802.971 kb on - strand, within PP_5081 at 5802.972 kb on + strand, within PP_5081 at 5802.972 kb on + strand, within PP_5081 at 5802.972 kb on + strand, within PP_5081 at 5802.972 kb on + strand, within PP_5081 at 5802.972 kb on + strand, within PP_5081 at 5802.973 kb on - strand, within PP_5081 at 5802.973 kb on - strand, within PP_5081 at 5802.973 kb on - strand, within PP_5081 at 5802.973 kb on - strand, within PP_5081 at 5802.973 kb on - strand, within PP_5081 at 5803.230 kb on + strand, within PP_5081 at 5803.231 kb on - strand, within PP_5081 at 5803.399 kb on + strand, within PP_5081 at 5803.435 kb on + strand, within PP_5081 at 5803.435 kb on + strand, within PP_5081 at 5803.436 kb on - strand, within PP_5081 at 5803.436 kb on - strand, within PP_5081 at 5803.526 kb on + strand, within PP_5081 at 5803.526 kb on + strand, within PP_5081 at 5803.567 kb on + strand, within PP_5081 at 5803.568 kb on - strand, within PP_5081 at 5803.568 kb on - strand, within PP_5081 at 5803.666 kb on + strand, within PP_5081 at 5803.666 kb on + strand, within PP_5081 at 5803.667 kb on - strand, within PP_5081
Per-strain Table
Position Strand Gene LocusTag Fraction LB outgrowth control more dilute start remove 5,800,965 - -1.0 5,800,978 - +0.4 5,801,062 + -1.5 5,801,062 + -0.1 5,801,674 - +0.8 5,801,704 + -0.7 5,801,704 + -2.5 5,801,705 - +0.6 5,801,705 - -0.1 5,801,838 + PP_5080 0.20 -1.3 5,801,839 - PP_5080 0.20 +0.2 5,801,928 + PP_5080 0.27 -0.4 5,801,929 - PP_5080 0.27 +0.4 5,801,995 + PP_5080 0.32 +1.1 5,802,215 - PP_5080 0.50 +1.4 5,802,254 - PP_5080 0.53 +0.8 5,802,275 + PP_5080 0.55 +0.6 5,802,685 - PP_5080 0.88 +0.9 5,802,685 - PP_5080 0.88 +0.7 5,802,685 - PP_5080 0.88 +0.1 5,802,718 + +0.2 5,802,719 - +0.1 5,802,838 + -0.0 5,802,839 - +0.3 5,802,839 - +0.1 5,802,839 - +1.1 5,802,970 + PP_5081 0.19 +0.6 5,802,970 + PP_5081 0.19 -0.1 5,802,970 + PP_5081 0.19 -0.1 5,802,970 + PP_5081 0.19 -0.3 5,802,970 + PP_5081 0.19 -0.1 5,802,971 - PP_5081 0.19 -1.3 5,802,971 - PP_5081 0.19 +0.4 5,802,971 - PP_5081 0.19 +0.2 5,802,971 - PP_5081 0.19 -1.2 5,802,971 - PP_5081 0.19 +0.3 5,802,971 - PP_5081 0.19 -0.7 5,802,971 - PP_5081 0.19 -0.9 5,802,971 - PP_5081 0.19 +0.8 5,802,971 - PP_5081 0.19 +0.2 5,802,971 - PP_5081 0.19 -0.2 5,802,971 - PP_5081 0.19 -0.2 5,802,972 + PP_5081 0.19 +0.9 5,802,972 + PP_5081 0.19 -0.0 5,802,972 + PP_5081 0.19 +0.0 5,802,972 + PP_5081 0.19 -0.2 5,802,972 + PP_5081 0.19 +0.0 5,802,973 - PP_5081 0.19 -0.4 5,802,973 - PP_5081 0.19 +0.7 5,802,973 - PP_5081 0.19 +0.0 5,802,973 - PP_5081 0.19 -0.1 5,802,973 - PP_5081 0.19 +0.7 5,803,230 + PP_5081 0.44 +0.5 5,803,231 - PP_5081 0.44 +0.3 5,803,399 + PP_5081 0.61 +0.6 5,803,435 + PP_5081 0.64 +1.2 5,803,435 + PP_5081 0.64 +0.7 5,803,436 - PP_5081 0.64 -0.8 5,803,436 - PP_5081 0.64 +0.1 5,803,526 + PP_5081 0.73 -0.7 5,803,526 + PP_5081 0.73 -1.3 5,803,567 + PP_5081 0.77 +0.9 5,803,568 - PP_5081 0.77 -0.4 5,803,568 - PP_5081 0.77 -0.3 5,803,666 + PP_5081 0.87 +0.6 5,803,666 + PP_5081 0.87 -0.0 5,803,667 - PP_5081 0.87 -0.8
Or see this region's nucleotide sequence