Experiment: LB outgrowth control more dilute start
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_2001 and PP_2002 overlap by 4 nucleotides PP_2002 and PP_t31 are separated by 219 nucleotides PP_t31 and PP_t32 are separated by 65 nucleotides PP_t32 and PP_t33 are separated by 99 nucleotides PP_t33 and PP_t34 are separated by 141 nucleotides PP_t34 and PP_2003 are separated by 83 nucleotides
PP_2001: PP_2001 - O-succinylhomoserine sulfhydrylase, at 2,269,198 to 2,270,409
_2001
PP_2002: PP_2002 - putative enzyme, at 2,270,406 to 2,271,179
_2002
PP_t31: PP_t31 - tRNA-Val, at 2,271,399 to 2,271,474
_t31
PP_t32: PP_t32 - tRNA-Asp, at 2,271,540 to 2,271,616
_t32
PP_t33: PP_t33 - tRNA-Asp, at 2,271,716 to 2,271,792
_t33
PP_t34: PP_t34 - tRNA-Asp, at 2,271,934 to 2,272,010
_t34
PP_2003: PP_2003 - conserved protein of unknown function, at 2,272,094 to 2,273,035
_2003
Position (kb)
2270
2271
2272 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2269.406 kb on - strand, within PP_2001 at 2269.477 kb on - strand, within PP_2001 at 2269.477 kb on - strand, within PP_2001 at 2269.591 kb on + strand, within PP_2001 at 2269.591 kb on + strand, within PP_2001 at 2269.591 kb on + strand, within PP_2001 at 2269.592 kb on - strand, within PP_2001 at 2269.592 kb on - strand, within PP_2001 at 2269.592 kb on - strand, within PP_2001 at 2269.592 kb on - strand, within PP_2001 at 2269.610 kb on + strand, within PP_2001 at 2269.611 kb on - strand, within PP_2001 at 2269.611 kb on - strand, within PP_2001 at 2269.621 kb on + strand, within PP_2001 at 2269.725 kb on + strand, within PP_2001 at 2269.726 kb on - strand, within PP_2001 at 2269.903 kb on - strand, within PP_2001 at 2270.010 kb on + strand, within PP_2001 at 2270.011 kb on - strand, within PP_2001 at 2270.011 kb on - strand, within PP_2001 at 2270.086 kb on + strand, within PP_2001 at 2270.087 kb on - strand, within PP_2001 at 2270.319 kb on + strand at 2270.805 kb on + strand, within PP_2002 at 2270.806 kb on - strand, within PP_2002 at 2270.847 kb on - strand, within PP_2002 at 2270.884 kb on - strand, within PP_2002 at 2270.884 kb on - strand, within PP_2002 at 2271.171 kb on + strand at 2271.171 kb on + strand at 2271.171 kb on + strand at 2271.171 kb on + strand at 2271.171 kb on + strand at 2271.171 kb on + strand at 2271.171 kb on + strand at 2271.171 kb on + strand at 2271.172 kb on - strand at 2271.172 kb on - strand at 2271.172 kb on - strand at 2271.172 kb on - strand at 2271.172 kb on - strand at 2271.172 kb on - strand at 2271.172 kb on - strand at 2271.172 kb on - strand at 2271.173 kb on + strand at 2271.173 kb on + strand at 2271.174 kb on - strand at 2271.227 kb on + strand at 2271.228 kb on - strand at 2271.236 kb on + strand at 2271.249 kb on + strand at 2271.249 kb on + strand at 2271.250 kb on - strand at 2271.724 kb on - strand, within PP_t33 at 2271.811 kb on + strand at 2271.812 kb on - strand at 2271.812 kb on - strand at 2271.896 kb on - strand at 2271.896 kb on - strand at 2271.942 kb on - strand, within PP_t34 at 2272.179 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB outgrowth control more dilute start remove 2,269,406 - PP_2001 0.17 -0.1 2,269,477 - PP_2001 0.23 -0.2 2,269,477 - PP_2001 0.23 +0.8 2,269,591 + PP_2001 0.32 +0.1 2,269,591 + PP_2001 0.32 +0.2 2,269,591 + PP_2001 0.32 -0.2 2,269,592 - PP_2001 0.33 -1.9 2,269,592 - PP_2001 0.33 -1.0 2,269,592 - PP_2001 0.33 -0.3 2,269,592 - PP_2001 0.33 -0.2 2,269,610 + PP_2001 0.34 +0.1 2,269,611 - PP_2001 0.34 -0.4 2,269,611 - PP_2001 0.34 +0.2 2,269,621 + PP_2001 0.35 -0.1 2,269,725 + PP_2001 0.43 +0.4 2,269,726 - PP_2001 0.44 -1.4 2,269,903 - PP_2001 0.58 -0.5 2,270,010 + PP_2001 0.67 +0.2 2,270,011 - PP_2001 0.67 -0.9 2,270,011 - PP_2001 0.67 -0.7 2,270,086 + PP_2001 0.73 -0.0 2,270,087 - PP_2001 0.73 -0.8 2,270,319 + -0.4 2,270,805 + PP_2002 0.52 +0.3 2,270,806 - PP_2002 0.52 +0.7 2,270,847 - PP_2002 0.57 +0.7 2,270,884 - PP_2002 0.62 +0.3 2,270,884 - PP_2002 0.62 +0.2 2,271,171 + -0.9 2,271,171 + -1.3 2,271,171 + -0.3 2,271,171 + +0.0 2,271,171 + +0.2 2,271,171 + +0.2 2,271,171 + +0.2 2,271,171 + +0.2 2,271,172 - +2.3 2,271,172 - -1.0 2,271,172 - -0.7 2,271,172 - +1.0 2,271,172 - -0.4 2,271,172 - +0.7 2,271,172 - +0.4 2,271,172 - +0.8 2,271,173 + +1.4 2,271,173 + +0.7 2,271,174 - -0.2 2,271,227 + -0.3 2,271,228 - -1.8 2,271,236 + -0.2 2,271,249 + -0.6 2,271,249 + -0.9 2,271,250 - +0.7 2,271,724 - PP_t33 0.10 +3.3 2,271,811 + -0.5 2,271,812 - +0.3 2,271,812 - -0.7 2,271,896 - -0.3 2,271,896 - -1.0 2,271,942 - PP_t34 0.10 +0.4 2,272,179 + +0.5
Or see this region's nucleotide sequence