Experiment: LB outgrowth control more dilute start
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0769 and PP_0770 are separated by 193 nucleotides PP_0770 and PP_0771 overlap by 4 nucleotides PP_0771 and PP_0772 are separated by 18 nucleotides PP_0772 and PP_0773 are separated by 118 nucleotides
PP_0769: PP_0769 - Sensor histidine kinase, at 888,571 to 889,263
_0769
PP_0770: PP_0770 - PemI-like protein, at 889,457 to 889,711
_0770
PP_0771: PP_0771 - mRNA interferase, at 889,708 to 890,055
_0771
PP_0772: PP_0772 - Metallo-beta-lactamase family protein, at 890,074 to 890,718
_0772
PP_0773: PP_0773 - OmpA/MotB domain-containing protein, at 890,837 to 891,547
_0773
Position (kb) 889
890
891 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 888.779 kb on - strand, within PP_0769 at 888.779 kb on - strand, within PP_0769 at 888.806 kb on + strand, within PP_0769 at 888.806 kb on + strand, within PP_0769 at 888.817 kb on + strand, within PP_0769 at 888.869 kb on + strand, within PP_0769 at 888.869 kb on + strand, within PP_0769 at 888.870 kb on - strand, within PP_0769 at 888.870 kb on - strand, within PP_0769 at 889.103 kb on - strand, within PP_0769 at 889.103 kb on - strand, within PP_0769 at 889.103 kb on - strand, within PP_0769 at 889.151 kb on - strand, within PP_0769 at 889.211 kb on + strand at 889.211 kb on + strand at 889.211 kb on + strand at 889.257 kb on - strand at 889.278 kb on + strand at 889.278 kb on + strand at 889.324 kb on + strand at 889.324 kb on + strand at 889.382 kb on + strand at 889.383 kb on - strand at 889.383 kb on - strand at 889.383 kb on - strand at 889.383 kb on - strand at 889.387 kb on - strand at 889.430 kb on - strand at 889.430 kb on - strand at 889.432 kb on + strand at 889.432 kb on + strand at 889.432 kb on + strand at 889.432 kb on + strand at 889.432 kb on + strand at 889.432 kb on + strand at 889.433 kb on - strand at 889.433 kb on - strand at 889.433 kb on - strand at 889.433 kb on - strand at 889.433 kb on - strand at 889.433 kb on - strand at 889.433 kb on - strand at 889.433 kb on - strand at 889.433 kb on - strand at 889.436 kb on + strand at 889.436 kb on + strand at 889.436 kb on + strand at 889.436 kb on + strand at 889.436 kb on + strand at 889.436 kb on + strand at 889.436 kb on + strand at 889.437 kb on - strand at 889.437 kb on - strand at 889.437 kb on - strand at 889.437 kb on - strand at 889.437 kb on - strand at 889.437 kb on - strand at 889.437 kb on - strand at 889.437 kb on - strand at 889.437 kb on - strand at 889.437 kb on - strand at 889.440 kb on + strand at 889.441 kb on - strand at 889.452 kb on + strand at 889.453 kb on - strand at 889.453 kb on - strand at 889.453 kb on - strand at 889.515 kb on + strand, within PP_0770 at 889.610 kb on + strand, within PP_0770 at 889.611 kb on - strand, within PP_0770 at 889.611 kb on - strand, within PP_0770 at 889.805 kb on + strand, within PP_0771 at 889.806 kb on - strand, within PP_0771 at 889.810 kb on - strand, within PP_0771 at 889.844 kb on + strand, within PP_0771 at 889.845 kb on - strand, within PP_0771 at 889.845 kb on - strand, within PP_0771 at 889.991 kb on + strand, within PP_0771 at 889.992 kb on - strand, within PP_0771 at 889.992 kb on - strand, within PP_0771 at 890.067 kb on - strand at 890.079 kb on + strand at 890.079 kb on + strand at 890.079 kb on + strand at 890.079 kb on + strand at 890.080 kb on - strand at 890.080 kb on - strand at 890.392 kb on + strand, within PP_0772 at 890.392 kb on + strand, within PP_0772 at 890.393 kb on - strand, within PP_0772 at 890.393 kb on - strand, within PP_0772 at 890.393 kb on - strand, within PP_0772 at 890.469 kb on + strand, within PP_0772 at 890.469 kb on + strand, within PP_0772 at 890.470 kb on - strand, within PP_0772 at 890.470 kb on - strand, within PP_0772 at 890.777 kb on + strand at 890.777 kb on + strand at 890.777 kb on + strand at 890.777 kb on + strand at 890.777 kb on + strand at 890.777 kb on + strand at 890.777 kb on + strand at 890.777 kb on + strand at 890.778 kb on - strand at 890.778 kb on - strand at 890.778 kb on - strand at 890.778 kb on - strand at 890.778 kb on - strand at 890.816 kb on - strand at 890.903 kb on + strand at 890.904 kb on - strand at 890.957 kb on + strand, within PP_0773
Per-strain Table
Position Strand Gene LocusTag Fraction LB outgrowth control more dilute start remove 888,779 - PP_0769 0.30 +0.8 888,779 - PP_0769 0.30 -0.4 888,806 + PP_0769 0.34 +0.9 888,806 + PP_0769 0.34 +0.8 888,817 + PP_0769 0.35 -0.5 888,869 + PP_0769 0.43 +0.8 888,869 + PP_0769 0.43 -0.8 888,870 - PP_0769 0.43 +0.3 888,870 - PP_0769 0.43 +0.6 889,103 - PP_0769 0.77 +0.8 889,103 - PP_0769 0.77 -0.5 889,103 - PP_0769 0.77 +0.2 889,151 - PP_0769 0.84 -0.4 889,211 + +0.1 889,211 + +0.1 889,211 + -0.5 889,257 - +0.5 889,278 + -0.3 889,278 + +0.0 889,324 + +0.3 889,324 + -0.2 889,382 + +0.3 889,383 - -0.7 889,383 - +2.1 889,383 - -0.0 889,383 - +0.2 889,387 - +0.3 889,430 - -2.8 889,430 - +0.3 889,432 + -0.6 889,432 + -0.9 889,432 + +1.1 889,432 + -0.8 889,432 + +0.0 889,432 + +0.2 889,433 - +0.2 889,433 - +0.2 889,433 - +0.2 889,433 - -0.5 889,433 - +1.1 889,433 - +0.5 889,433 - -0.7 889,433 - +0.2 889,433 - +0.9 889,436 + -0.2 889,436 + +0.1 889,436 + +1.0 889,436 + -0.3 889,436 + -2.3 889,436 + +0.7 889,436 + -0.2 889,437 - +0.2 889,437 - +0.5 889,437 - -0.3 889,437 - +0.7 889,437 - -0.1 889,437 - +0.3 889,437 - -0.1 889,437 - -0.0 889,437 - +0.4 889,437 - -0.1 889,440 + +0.2 889,441 - -0.9 889,452 + +0.1 889,453 - +0.1 889,453 - -0.1 889,453 - +0.4 889,515 + PP_0770 0.23 -0.3 889,610 + PP_0770 0.60 -0.3 889,611 - PP_0770 0.60 +0.4 889,611 - PP_0770 0.60 -0.2 889,805 + PP_0771 0.28 +0.3 889,806 - PP_0771 0.28 +0.4 889,810 - PP_0771 0.29 +0.5 889,844 + PP_0771 0.39 -0.2 889,845 - PP_0771 0.39 +1.0 889,845 - PP_0771 0.39 +0.9 889,991 + PP_0771 0.81 -0.2 889,992 - PP_0771 0.82 -0.1 889,992 - PP_0771 0.82 +0.6 890,067 - -0.1 890,079 + +0.2 890,079 + +2.4 890,079 + -0.6 890,079 + +0.4 890,080 - +0.7 890,080 - -0.0 890,392 + PP_0772 0.49 -1.2 890,392 + PP_0772 0.49 +1.2 890,393 - PP_0772 0.49 -0.9 890,393 - PP_0772 0.49 -0.2 890,393 - PP_0772 0.49 -0.4 890,469 + PP_0772 0.61 +0.1 890,469 + PP_0772 0.61 -0.1 890,470 - PP_0772 0.61 +0.1 890,470 - PP_0772 0.61 -0.5 890,777 + -0.9 890,777 + +0.1 890,777 + +0.2 890,777 + +0.3 890,777 + +0.5 890,777 + +0.9 890,777 + -0.4 890,777 + -0.3 890,778 - +0.0 890,778 - +0.9 890,778 - +0.0 890,778 - -1.3 890,778 - +0.7 890,816 - +1.2 890,903 + -0.6 890,904 - -0.6 890,957 + PP_0773 0.17 -0.2
Or see this region's nucleotide sequence