Experiment: LB outgrowth control more dilute start
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_2501 and PP_2502 are separated by 522 nucleotides PP_2502 and PP_2503 are separated by 113 nucleotides PP_2503 and PP_2504 are separated by 69 nucleotides
PP_2501: PP_2501 - putative phage integrase, at 2,847,237 to 2,848,646
_2501
PP_2502: PP_2502 - Transcriptional regulator, LysR family, at 2,849,169 to 2,850,095
_2502
PP_2503: PP_2503 - putative 3-oxoacyl-(Acyl-carrier-protein) reductase, at 2,850,209 to 2,850,940
_2503
PP_2504: PP_2504 - putative tautomerase K2, at 2,851,010 to 2,851,219
_2504
Position (kb)
2849
2850
2851 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2848.172 kb on + strand, within PP_2501 at 2848.172 kb on + strand, within PP_2501 at 2848.173 kb on - strand, within PP_2501 at 2848.209 kb on + strand, within PP_2501 at 2848.209 kb on + strand, within PP_2501 at 2848.210 kb on - strand, within PP_2501 at 2848.210 kb on - strand, within PP_2501 at 2848.210 kb on - strand, within PP_2501 at 2848.272 kb on + strand, within PP_2501 at 2848.337 kb on + strand, within PP_2501 at 2848.338 kb on - strand at 2848.473 kb on - strand, within PP_2501 at 2848.488 kb on - strand, within PP_2501 at 2848.516 kb on + strand at 2848.516 kb on + strand at 2848.636 kb on - strand at 2848.651 kb on + strand at 2848.739 kb on - strand at 2848.770 kb on - strand at 2848.784 kb on - strand at 2848.784 kb on - strand at 2848.796 kb on - strand at 2848.813 kb on - strand at 2848.822 kb on + strand at 2848.829 kb on + strand at 2848.883 kb on + strand at 2848.883 kb on + strand at 2848.884 kb on - strand at 2848.884 kb on - strand at 2848.961 kb on + strand at 2848.961 kb on + strand at 2848.962 kb on - strand at 2849.000 kb on - strand at 2849.013 kb on - strand at 2849.013 kb on - strand at 2849.082 kb on + strand at 2849.109 kb on + strand at 2849.109 kb on + strand at 2849.109 kb on + strand at 2849.110 kb on - strand at 2849.110 kb on - strand at 2849.110 kb on - strand at 2849.110 kb on - strand at 2849.110 kb on - strand at 2849.110 kb on - strand at 2849.258 kb on - strand at 2849.350 kb on + strand, within PP_2502 at 2849.350 kb on + strand, within PP_2502 at 2849.420 kb on - strand, within PP_2502 at 2849.567 kb on - strand, within PP_2502 at 2849.567 kb on - strand, within PP_2502 at 2849.567 kb on - strand, within PP_2502 at 2849.567 kb on - strand, within PP_2502 at 2849.774 kb on - strand, within PP_2502 at 2849.774 kb on - strand, within PP_2502 at 2849.774 kb on - strand, within PP_2502 at 2849.881 kb on + strand, within PP_2502 at 2849.881 kb on + strand, within PP_2502 at 2849.881 kb on + strand, within PP_2502 at 2849.881 kb on + strand, within PP_2502 at 2849.882 kb on - strand, within PP_2502 at 2849.882 kb on - strand, within PP_2502 at 2850.137 kb on + strand at 2850.143 kb on + strand at 2850.159 kb on - strand at 2850.501 kb on - strand, within PP_2503 at 2850.555 kb on - strand, within PP_2503 at 2850.555 kb on - strand, within PP_2503 at 2850.867 kb on - strand at 2850.867 kb on - strand at 2850.867 kb on - strand at 2850.919 kb on + strand at 2850.920 kb on - strand at 2850.920 kb on - strand at 2850.938 kb on + strand at 2850.938 kb on + strand at 2850.941 kb on + strand at 2850.941 kb on + strand at 2850.941 kb on + strand at 2850.942 kb on - strand at 2850.942 kb on - strand at 2850.942 kb on - strand at 2851.003 kb on + strand at 2851.003 kb on + strand at 2851.003 kb on + strand at 2851.003 kb on + strand at 2851.003 kb on + strand at 2851.004 kb on - strand at 2851.004 kb on - strand at 2851.004 kb on - strand at 2851.006 kb on + strand at 2851.006 kb on + strand at 2851.006 kb on + strand at 2851.007 kb on - strand at 2851.007 kb on - strand at 2851.007 kb on - strand at 2851.007 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB outgrowth control more dilute start remove 2,848,172 + PP_2501 0.66 -0.7 2,848,172 + PP_2501 0.66 -0.1 2,848,173 - PP_2501 0.66 -1.0 2,848,209 + PP_2501 0.69 +0.6 2,848,209 + PP_2501 0.69 +0.0 2,848,210 - PP_2501 0.69 -0.5 2,848,210 - PP_2501 0.69 +0.3 2,848,210 - PP_2501 0.69 +0.1 2,848,272 + PP_2501 0.73 -1.3 2,848,337 + PP_2501 0.78 -0.8 2,848,338 - +0.6 2,848,473 - PP_2501 0.88 -0.5 2,848,488 - PP_2501 0.89 -0.2 2,848,516 + +0.1 2,848,516 + +0.6 2,848,636 - +0.0 2,848,651 + -0.1 2,848,739 - +0.4 2,848,770 - -0.3 2,848,784 - -0.2 2,848,784 - -0.2 2,848,796 - -0.4 2,848,813 - -0.7 2,848,822 + -0.2 2,848,829 + -1.1 2,848,883 + -1.4 2,848,883 + +0.1 2,848,884 - -0.3 2,848,884 - -0.1 2,848,961 + +0.4 2,848,961 + -0.9 2,848,962 - +0.6 2,849,000 - +0.5 2,849,013 - +1.2 2,849,013 - -1.1 2,849,082 + -1.0 2,849,109 + -0.0 2,849,109 + +0.4 2,849,109 + +1.0 2,849,110 - -0.2 2,849,110 - +0.8 2,849,110 - +0.2 2,849,110 - +0.4 2,849,110 - -0.1 2,849,110 - +0.1 2,849,258 - -1.8 2,849,350 + PP_2502 0.20 +0.7 2,849,350 + PP_2502 0.20 +0.4 2,849,420 - PP_2502 0.27 +0.4 2,849,567 - PP_2502 0.43 +0.3 2,849,567 - PP_2502 0.43 -0.3 2,849,567 - PP_2502 0.43 +0.3 2,849,567 - PP_2502 0.43 +0.8 2,849,774 - PP_2502 0.65 +0.7 2,849,774 - PP_2502 0.65 +0.5 2,849,774 - PP_2502 0.65 -0.5 2,849,881 + PP_2502 0.77 +0.8 2,849,881 + PP_2502 0.77 +1.0 2,849,881 + PP_2502 0.77 -0.7 2,849,881 + PP_2502 0.77 +0.2 2,849,882 - PP_2502 0.77 -1.5 2,849,882 - PP_2502 0.77 +1.9 2,850,137 + +0.8 2,850,143 + +0.4 2,850,159 - -0.3 2,850,501 - PP_2503 0.40 +0.7 2,850,555 - PP_2503 0.47 -0.2 2,850,555 - PP_2503 0.47 -0.1 2,850,867 - -0.0 2,850,867 - +1.1 2,850,867 - +0.8 2,850,919 + -0.2 2,850,920 - -0.3 2,850,920 - -0.8 2,850,938 + +0.4 2,850,938 + +0.1 2,850,941 + -0.6 2,850,941 + -0.3 2,850,941 + -0.5 2,850,942 - +0.4 2,850,942 - +0.2 2,850,942 - -0.5 2,851,003 + +0.2 2,851,003 + -1.0 2,851,003 + -0.3 2,851,003 + -0.4 2,851,003 + -0.3 2,851,004 - +0.3 2,851,004 - -0.7 2,851,004 - -0.3 2,851,006 + -1.0 2,851,006 + -0.4 2,851,006 + -0.1 2,851,007 - +0.7 2,851,007 - -0.8 2,851,007 - -0.7 2,851,007 - +1.2
Or see this region's nucleotide sequence