Experiment: LB outgrowth control more dilute start
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_1027 and PP_1028 are separated by 94 nucleotides PP_1028 and PP_1029 are separated by 97 nucleotides PP_1029 and PP_1030 are separated by 47 nucleotides
PP_1027: PP_1027 - Exodeoxyribonuclease 7 large subunit, at 1,171,673 to 1,173,052
_1027
PP_1028: PP_1028 - Transcriptional regulator, LysR family, at 1,173,147 to 1,174,025
_1028
PP_1029: PP_1029 - conserved membrane protein of unknown function, at 1,174,123 to 1,174,878
_1029
PP_1030: PP_1030 - conserved protein of unknown function, at 1,174,926 to 1,175,483
_1030
Position (kb)
1173
1174
1175 Strain fitness (log2 ratio)
-2
-1
0
1 at 1172.507 kb on + strand, within PP_1027 at 1172.515 kb on + strand, within PP_1027 at 1172.516 kb on - strand, within PP_1027 at 1172.519 kb on + strand, within PP_1027 at 1172.520 kb on - strand, within PP_1027 at 1172.743 kb on + strand, within PP_1027 at 1172.744 kb on - strand, within PP_1027 at 1172.744 kb on - strand, within PP_1027 at 1172.744 kb on - strand, within PP_1027 at 1172.744 kb on - strand, within PP_1027 at 1172.744 kb on - strand, within PP_1027 at 1172.751 kb on + strand, within PP_1027 at 1172.752 kb on - strand, within PP_1027 at 1172.892 kb on + strand, within PP_1027 at 1172.892 kb on + strand, within PP_1027 at 1172.893 kb on - strand, within PP_1027 at 1172.893 kb on - strand, within PP_1027 at 1172.893 kb on - strand, within PP_1027 at 1173.005 kb on - strand at 1173.064 kb on + strand at 1173.064 kb on + strand at 1173.064 kb on + strand at 1173.065 kb on - strand at 1173.065 kb on - strand at 1173.099 kb on + strand at 1173.315 kb on + strand, within PP_1028 at 1173.316 kb on - strand, within PP_1028 at 1173.403 kb on + strand, within PP_1028 at 1173.403 kb on + strand, within PP_1028 at 1173.404 kb on - strand, within PP_1028 at 1173.682 kb on + strand, within PP_1028 at 1173.946 kb on + strand at 1173.946 kb on + strand at 1173.947 kb on - strand at 1174.063 kb on + strand at 1174.063 kb on + strand at 1174.066 kb on + strand at 1174.066 kb on + strand at 1174.090 kb on + strand at 1174.189 kb on + strand at 1174.189 kb on + strand at 1174.190 kb on - strand at 1174.190 kb on - strand at 1174.219 kb on - strand, within PP_1029 at 1174.389 kb on + strand, within PP_1029 at 1174.390 kb on - strand, within PP_1029 at 1174.390 kb on - strand, within PP_1029 at 1174.390 kb on - strand, within PP_1029 at 1174.465 kb on + strand, within PP_1029 at 1174.466 kb on - strand, within PP_1029 at 1174.466 kb on - strand, within PP_1029 at 1174.474 kb on + strand, within PP_1029 at 1174.585 kb on + strand, within PP_1029 at 1174.595 kb on - strand, within PP_1029 at 1174.629 kb on - strand, within PP_1029 at 1174.629 kb on - strand, within PP_1029 at 1174.629 kb on - strand, within PP_1029 at 1174.662 kb on + strand, within PP_1029 at 1174.663 kb on - strand, within PP_1029 at 1174.668 kb on - strand, within PP_1029 at 1174.702 kb on + strand, within PP_1029 at 1174.703 kb on - strand, within PP_1029 at 1174.703 kb on - strand, within PP_1029 at 1174.864 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB outgrowth control more dilute start remove 1,172,507 + PP_1027 0.60 +0.1 1,172,515 + PP_1027 0.61 +0.5 1,172,516 - PP_1027 0.61 -0.7 1,172,519 + PP_1027 0.61 -0.5 1,172,520 - PP_1027 0.61 +0.6 1,172,743 + PP_1027 0.78 -0.2 1,172,744 - PP_1027 0.78 -1.3 1,172,744 - PP_1027 0.78 +1.2 1,172,744 - PP_1027 0.78 +1.1 1,172,744 - PP_1027 0.78 +0.5 1,172,744 - PP_1027 0.78 +0.3 1,172,751 + PP_1027 0.78 -0.2 1,172,752 - PP_1027 0.78 -0.2 1,172,892 + PP_1027 0.88 -0.6 1,172,892 + PP_1027 0.88 -1.1 1,172,893 - PP_1027 0.88 +0.5 1,172,893 - PP_1027 0.88 -1.8 1,172,893 - PP_1027 0.88 +0.4 1,173,005 - +0.2 1,173,064 + -1.0 1,173,064 + +0.8 1,173,064 + +0.1 1,173,065 - +0.4 1,173,065 - +0.2 1,173,099 + -0.3 1,173,315 + PP_1028 0.19 +0.9 1,173,316 - PP_1028 0.19 +0.3 1,173,403 + PP_1028 0.29 +0.4 1,173,403 + PP_1028 0.29 +1.5 1,173,404 - PP_1028 0.29 -1.2 1,173,682 + PP_1028 0.61 +0.6 1,173,946 + +1.0 1,173,946 + -0.3 1,173,947 - -0.3 1,174,063 + -0.5 1,174,063 + -0.3 1,174,066 + -0.3 1,174,066 + -0.3 1,174,090 + -0.2 1,174,189 + -0.9 1,174,189 + +0.4 1,174,190 - +0.4 1,174,190 - -0.1 1,174,219 - PP_1029 0.13 -0.6 1,174,389 + PP_1029 0.35 -2.4 1,174,390 - PP_1029 0.35 +0.6 1,174,390 - PP_1029 0.35 -0.2 1,174,390 - PP_1029 0.35 +0.4 1,174,465 + PP_1029 0.45 +0.1 1,174,466 - PP_1029 0.45 +0.4 1,174,466 - PP_1029 0.45 -0.7 1,174,474 + PP_1029 0.46 +0.2 1,174,585 + PP_1029 0.61 +0.2 1,174,595 - PP_1029 0.62 -0.0 1,174,629 - PP_1029 0.67 -0.5 1,174,629 - PP_1029 0.67 -0.2 1,174,629 - PP_1029 0.67 +0.4 1,174,662 + PP_1029 0.71 +0.5 1,174,663 - PP_1029 0.71 -0.1 1,174,668 - PP_1029 0.72 +0.0 1,174,702 + PP_1029 0.77 -0.4 1,174,703 - PP_1029 0.77 +0.2 1,174,703 - PP_1029 0.77 +0.1 1,174,864 + -1.1
Or see this region's nucleotide sequence