Experiment: 80% Methanol 2 mM; solid stress
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_00365 and PS417_00370 are separated by 98 nucleotides PS417_00370 and PS417_00375 are separated by 37 nucleotides PS417_00375 and PS417_00380 overlap by 8 nucleotides PS417_00380 and PS417_00385 overlap by 1 nucleotides
PS417_00365: PS417_00365 - hydroperoxidase, at 70,467 to 72,608
_00365
PS417_00370: PS417_00370 - hypothetical protein, at 72,707 to 73,426
_00370
PS417_00375: PS417_00375 - membrane protein, at 73,464 to 74,252
_00375
PS417_00380: PS417_00380 - zinc ABC transporter ATP-binding protein, at 74,245 to 75,030
_00380
PS417_00385: PS417_00385 - Fur family transcriptional regulator, at 75,030 to 75,512
_00385
Position (kb) 73
74
75 Strain fitness (log2 ratio) -1
0
1 at 72.584 kb on + strand at 72.585 kb on - strand at 72.591 kb on - strand at 73.183 kb on - strand, within PS417_00370 at 73.440 kb on + strand at 73.465 kb on + strand at 73.887 kb on - strand, within PS417_00375 at 73.913 kb on - strand, within PS417_00375 at 74.155 kb on + strand, within PS417_00375 at 74.262 kb on + strand at 74.348 kb on + strand, within PS417_00380 at 74.550 kb on - strand at 74.550 kb on - strand, within PS417_00380
Per-strain Table
Position Strand Gene LocusTag Fraction 80% Methanol 2 mM; solid stress remove 72,584 + +0.5 72,585 - +0.8 72,591 - -0.4 73,183 - PS417_00370 0.66 +0.1 73,440 + -0.1 73,465 + +0.8 73,887 - PS417_00375 0.54 -0.5 73,913 - PS417_00375 0.57 -0.4 74,155 + PS417_00375 0.88 -0.2 74,262 + -0.4 74,348 + PS417_00380 0.13 +0.4 74,550 - +1.0 74,550 - PS417_00380 0.39 -0.3
Or see this region's nucleotide sequence