Strain Fitness in Pseudomonas simiae WCS417 around PS417_15135

Experiment: 80% Methanol 2 mM; solid stress

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPS417_15120 and PS417_15125 overlap by 4 nucleotidesPS417_15125 and PS417_15135 are separated by 281 nucleotidesPS417_15135 and PS417_15140 are separated by 156 nucleotides PS417_15120: PS417_15120 - chromosome segregation protein SMC, at 3,307,470 to 3,311,108 _15120 PS417_15125: PS417_15125 - glutathione S-transferase, at 3,311,105 to 3,311,698 _15125 PS417_15135: PS417_15135 - hypothetical protein, at 3,311,980 to 3,312,159 _15135 PS417_15140: PS417_15140 - 3-carboxymuconate cyclase, at 3,312,316 to 3,313,491 _15140 Position (kb) 3311 3312 3313Strain fitness (log2 ratio) -2 -1 0 1at 3311.026 kb on + strandat 3311.123 kb on + strandat 3311.123 kb on + strandat 3311.124 kb on - strandat 3311.349 kb on + strand, within PS417_15125at 3311.409 kb on - strand, within PS417_15125at 3311.526 kb on - strand, within PS417_15125at 3311.696 kb on + strandat 3311.784 kb on - strandat 3312.047 kb on + strand, within PS417_15135at 3312.048 kb on - strand, within PS417_15135at 3312.267 kb on + strandat 3312.268 kb on - strandat 3312.590 kb on + strand, within PS417_15140at 3312.591 kb on - strand, within PS417_15140at 3312.861 kb on - strandat 3312.909 kb on - strand, within PS417_15140at 3313.012 kb on + strand, within PS417_15140at 3313.077 kb on - strand, within PS417_15140at 3313.077 kb on - strand, within PS417_15140

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Per-strain Table

Position Strand Gene LocusTag Fraction 80% Methanol 2 mM; solid stress
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3,311,026 + -0.3
3,311,123 + -0.3
3,311,123 + -0.8
3,311,124 - -0.5
3,311,349 + PS417_15125 0.41 +0.9
3,311,409 - PS417_15125 0.51 -0.0
3,311,526 - PS417_15125 0.71 +0.9
3,311,696 + +0.1
3,311,784 - -0.3
3,312,047 + PS417_15135 0.37 -0.4
3,312,048 - PS417_15135 0.38 -1.5
3,312,267 + +0.7
3,312,268 - -0.7
3,312,590 + PS417_15140 0.23 -2.1
3,312,591 - PS417_15140 0.23 -0.0
3,312,861 - +0.5
3,312,909 - PS417_15140 0.50 +0.3
3,313,012 + PS417_15140 0.59 -0.3
3,313,077 - PS417_15140 0.65 -0.4
3,313,077 - PS417_15140 0.65 +0.0

Or see this region's nucleotide sequence