Experiment: 80% Methanol 2 mM; solid stress
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_12515 and PS417_12520 are separated by 33 nucleotides PS417_12520 and PS417_12525 are separated by 163 nucleotides PS417_12525 and PS417_12530 overlap by 8 nucleotides PS417_12530 and PS417_12535 are separated by 81 nucleotides
PS417_12515: PS417_12515 - lactate dehydrogenase, at 2,724,045 to 2,725,082
_12515
PS417_12520: PS417_12520 - hypothetical protein, at 2,725,116 to 2,725,436
_12520
PS417_12525: PS417_12525 - hypothetical protein, at 2,725,600 to 2,725,854
_12525
PS417_12530: PS417_12530 - LysR family transcriptional regulator, at 2,725,847 to 2,726,740
_12530
PS417_12535: PS417_12535 - nitrilase, at 2,726,822 to 2,727,748
_12535
Position (kb)
2725
2726 Strain fitness (log2 ratio)
-1
0
1 at 2725.030 kb on + strand at 2725.122 kb on - strand at 2725.202 kb on - strand, within PS417_12520 at 2725.202 kb on - strand, within PS417_12520 at 2725.202 kb on - strand, within PS417_12520 at 2725.557 kb on + strand at 2725.557 kb on + strand at 2725.558 kb on - strand at 2725.558 kb on - strand at 2725.558 kb on - strand at 2725.558 kb on - strand at 2725.558 kb on - strand at 2725.564 kb on + strand at 2725.564 kb on + strand at 2725.565 kb on - strand at 2725.565 kb on - strand at 2725.565 kb on - strand at 2725.565 kb on - strand at 2725.565 kb on - strand at 2725.577 kb on - strand at 2725.592 kb on - strand at 2725.592 kb on - strand at 2725.669 kb on + strand, within PS417_12525 at 2725.669 kb on + strand, within PS417_12525 at 2725.669 kb on + strand, within PS417_12525 at 2725.744 kb on - strand, within PS417_12525 at 2725.852 kb on + strand at 2725.852 kb on + strand at 2725.853 kb on - strand at 2726.081 kb on + strand, within PS417_12530 at 2726.237 kb on + strand, within PS417_12530 at 2726.238 kb on - strand, within PS417_12530 at 2726.452 kb on - strand, within PS417_12530 at 2726.589 kb on + strand, within PS417_12530 at 2726.589 kb on + strand, within PS417_12530 at 2726.589 kb on + strand, within PS417_12530 at 2726.589 kb on + strand, within PS417_12530 at 2726.590 kb on - strand, within PS417_12530 at 2726.590 kb on - strand, within PS417_12530 at 2726.590 kb on - strand, within PS417_12530 at 2726.805 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 80% Methanol 2 mM; solid stress remove 2,725,030 + +0.5 2,725,122 - +1.1 2,725,202 - PS417_12520 0.27 +0.8 2,725,202 - PS417_12520 0.27 -0.1 2,725,202 - PS417_12520 0.27 +0.6 2,725,557 + +0.1 2,725,557 + -0.4 2,725,558 - -0.5 2,725,558 - -0.2 2,725,558 - -0.4 2,725,558 - +0.4 2,725,558 - -0.7 2,725,564 + -0.4 2,725,564 + -0.3 2,725,565 - -0.4 2,725,565 - -0.1 2,725,565 - -0.2 2,725,565 - -0.6 2,725,565 - +0.3 2,725,577 - -0.5 2,725,592 - -0.7 2,725,592 - -0.2 2,725,669 + PS417_12525 0.27 -0.7 2,725,669 + PS417_12525 0.27 -0.1 2,725,669 + PS417_12525 0.27 -0.5 2,725,744 - PS417_12525 0.56 -1.1 2,725,852 + -0.2 2,725,852 + -0.2 2,725,853 - +0.2 2,726,081 + PS417_12530 0.26 +0.1 2,726,237 + PS417_12530 0.44 +0.3 2,726,238 - PS417_12530 0.44 -0.1 2,726,452 - PS417_12530 0.68 +0.3 2,726,589 + PS417_12530 0.83 -0.0 2,726,589 + PS417_12530 0.83 -0.5 2,726,589 + PS417_12530 0.83 -0.5 2,726,589 + PS417_12530 0.83 -0.1 2,726,590 - PS417_12530 0.83 -0.3 2,726,590 - PS417_12530 0.83 +0.2 2,726,590 - PS417_12530 0.83 -0.7 2,726,805 + -0.7
Or see this region's nucleotide sequence