Strain Fitness in Pseudomonas simiae WCS417 around PS417_00025

Experiment: 80% Methanol 3 mM; liquid stress

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPS417_00020 and PS417_00025 are separated by 106 nucleotidesPS417_00025 and PS417_00030 are separated by 12 nucleotidesPS417_00030 and PS417_00035 are separated by 57 nucleotides PS417_00020: PS417_00020 - DNA gyrase subunit B, at 4,013 to 6,430 _00020 PS417_00025: PS417_00025 - transcriptional regulator, at 6,537 to 7,259 _00025 PS417_00030: PS417_00030 - hypothetical protein, at 7,272 to 7,457 _00030 PS417_00035: PS417_00035 - hypothetical protein, at 7,515 to 8,432 _00035 Position (kb) 6 7 8Strain fitness (log2 ratio) -2 -1 0 1 2at 6.451 kb on + strandat 6.539 kb on - strandat 6.539 kb on - strandat 6.581 kb on - strandat 6.586 kb on + strandat 6.586 kb on + strandat 6.587 kb on - strandat 6.587 kb on - strandat 6.587 kb on - strandat 6.587 kb on - strandat 6.587 kb on - strandat 6.587 kb on - strandat 6.598 kb on - strandat 6.646 kb on + strand, within PS417_00025at 6.650 kb on + strand, within PS417_00025at 6.650 kb on + strand, within PS417_00025at 6.650 kb on + strand, within PS417_00025at 6.650 kb on + strand, within PS417_00025at 6.650 kb on + strand, within PS417_00025at 6.650 kb on + strand, within PS417_00025at 6.650 kb on + strand, within PS417_00025at 6.650 kb on + strand, within PS417_00025at 6.650 kb on + strand, within PS417_00025at 6.650 kb on + strand, within PS417_00025at 6.650 kb on + strand, within PS417_00025at 6.650 kb on + strand, within PS417_00025at 6.650 kb on + strand, within PS417_00025at 6.650 kb on + strand, within PS417_00025at 6.650 kb on + strand, within PS417_00025at 6.651 kb on - strand, within PS417_00025at 6.651 kb on - strand, within PS417_00025at 6.651 kb on - strand, within PS417_00025at 6.651 kb on - strand, within PS417_00025at 6.651 kb on - strand, within PS417_00025at 6.651 kb on - strand, within PS417_00025at 6.651 kb on - strand, within PS417_00025at 6.712 kb on + strand, within PS417_00025at 6.755 kb on + strand, within PS417_00025at 6.946 kb on + strand, within PS417_00025at 6.947 kb on - strand, within PS417_00025at 6.947 kb on - strandat 7.095 kb on + strand, within PS417_00025at 7.355 kb on + strand, within PS417_00030at 7.356 kb on - strand, within PS417_00030at 7.356 kb on - strand, within PS417_00030at 7.357 kb on + strand, within PS417_00030at 7.543 kb on - strandat 7.543 kb on - strandat 7.543 kb on - strandat 7.543 kb on - strandat 7.620 kb on + strand, within PS417_00035at 7.621 kb on - strand, within PS417_00035at 7.621 kb on - strand, within PS417_00035at 7.908 kb on + strand, within PS417_00035at 7.909 kb on - strand, within PS417_00035at 7.909 kb on - strand, within PS417_00035at 8.202 kb on + strand, within PS417_00035at 8.203 kb on - strand, within PS417_00035

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Per-strain Table

Position Strand Gene LocusTag Fraction 80% Methanol 3 mM; liquid stress
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6,451 + -0.4
6,539 - -0.0
6,539 - +0.9
6,581 - +0.7
6,586 + +1.0
6,586 + +1.1
6,587 - +1.3
6,587 - +1.8
6,587 - +0.1
6,587 - +1.1
6,587 - +1.1
6,587 - -0.9
6,598 - +1.0
6,646 + PS417_00025 0.15 +0.5
6,650 + PS417_00025 0.16 +0.6
6,650 + PS417_00025 0.16 +0.8
6,650 + PS417_00025 0.16 +1.4
6,650 + PS417_00025 0.16 +0.5
6,650 + PS417_00025 0.16 +0.0
6,650 + PS417_00025 0.16 +1.0
6,650 + PS417_00025 0.16 +0.7
6,650 + PS417_00025 0.16 +1.0
6,650 + PS417_00025 0.16 +0.6
6,650 + PS417_00025 0.16 +0.8
6,650 + PS417_00025 0.16 +2.5
6,650 + PS417_00025 0.16 +1.2
6,650 + PS417_00025 0.16 +1.4
6,650 + PS417_00025 0.16 +1.2
6,650 + PS417_00025 0.16 +0.5
6,651 - PS417_00025 0.16 +2.1
6,651 - PS417_00025 0.16 +0.0
6,651 - PS417_00025 0.16 +1.9
6,651 - PS417_00025 0.16 +1.5
6,651 - PS417_00025 0.16 +0.8
6,651 - PS417_00025 0.16 +0.6
6,651 - PS417_00025 0.16 +0.6
6,712 + PS417_00025 0.24 +1.0
6,755 + PS417_00025 0.30 +1.7
6,946 + PS417_00025 0.57 +1.7
6,947 - PS417_00025 0.57 +1.0
6,947 - +0.3
7,095 + PS417_00025 0.77 +0.7
7,355 + PS417_00030 0.45 +1.2
7,356 - PS417_00030 0.45 +0.5
7,356 - PS417_00030 0.45 -0.2
7,357 + PS417_00030 0.46 +0.7
7,543 - -0.3
7,543 - -0.1
7,543 - +0.2
7,543 - -0.1
7,620 + PS417_00035 0.11 -0.2
7,621 - PS417_00035 0.12 -1.0
7,621 - PS417_00035 0.12 -0.6
7,908 + PS417_00035 0.43 -0.4
7,909 - PS417_00035 0.43 -0.5
7,909 - PS417_00035 0.43 +0.0
8,202 + PS417_00035 0.75 +0.0
8,203 - PS417_00035 0.75 -1.9

Or see this region's nucleotide sequence