Experiment: 20 hr rep2_No_BD100_grewON
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt tfaE and stfE overlap by 1 nucleotides stfE and pin are separated by 65 nucleotides pin and mcrA are separated by 106 nucleotides
b1156: tfaE - e14 prophage; predicted tail fiber assembly protein (NCBI), at 1,207,740 to 1,208,342
tfaE
b1157: stfE - no description, at 1,208,342 to 1,208,842
stfE
b1158: pin - e14 prophage; site-specific DNA recombinase (NCBI), at 1,208,908 to 1,209,462
pin
b1159: mcrA - e14 prophage; 5-methylcytosine-specific restriction endonuclease B (NCBI), at 1,209,569 to 1,210,402
mcrA
Position (kb)
1208
1209
1210 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1207.959 kb on + strand, within tfaE at 1207.962 kb on - strand, within tfaE at 1207.964 kb on - strand, within tfaE at 1207.967 kb on + strand, within tfaE at 1207.972 kb on + strand, within tfaE at 1208.100 kb on - strand, within tfaE at 1208.167 kb on - strand, within tfaE at 1208.167 kb on - strand, within tfaE at 1208.168 kb on - strand, within tfaE at 1208.168 kb on - strand, within tfaE at 1208.170 kb on + strand, within tfaE at 1208.172 kb on + strand, within tfaE at 1208.177 kb on + strand, within tfaE at 1208.178 kb on - strand, within tfaE at 1208.178 kb on - strand, within tfaE at 1208.180 kb on - strand, within tfaE at 1208.180 kb on - strand, within tfaE at 1208.184 kb on + strand, within tfaE at 1208.185 kb on - strand, within tfaE at 1208.187 kb on - strand, within tfaE at 1208.194 kb on + strand, within tfaE at 1208.194 kb on + strand, within tfaE at 1208.194 kb on + strand, within tfaE at 1208.352 kb on - strand at 1208.413 kb on - strand, within stfE at 1208.413 kb on - strand, within stfE at 1208.441 kb on - strand, within stfE at 1208.560 kb on - strand, within stfE at 1208.560 kb on - strand, within stfE at 1208.575 kb on - strand, within stfE at 1208.576 kb on - strand, within stfE at 1208.586 kb on - strand, within stfE at 1208.604 kb on - strand, within stfE at 1208.604 kb on - strand, within stfE at 1208.604 kb on - strand, within stfE at 1208.606 kb on - strand, within stfE at 1208.609 kb on - strand, within stfE at 1208.638 kb on - strand, within stfE at 1208.642 kb on - strand, within stfE at 1208.642 kb on - strand, within stfE at 1208.642 kb on - strand, within stfE at 1208.648 kb on + strand, within stfE at 1208.752 kb on + strand, within stfE at 1208.756 kb on - strand, within stfE at 1208.791 kb on - strand, within stfE at 1208.819 kb on + strand at 1208.838 kb on - strand at 1208.899 kb on - strand at 1208.946 kb on + strand at 1208.946 kb on + strand at 1208.964 kb on - strand, within pin at 1208.964 kb on - strand, within pin at 1208.968 kb on + strand, within pin at 1209.015 kb on - strand, within pin at 1209.110 kb on - strand, within pin at 1209.296 kb on + strand, within pin at 1209.296 kb on + strand, within pin at 1209.306 kb on + strand, within pin at 1209.306 kb on + strand at 1209.316 kb on + strand, within pin at 1209.316 kb on + strand, within pin at 1209.333 kb on + strand, within pin at 1209.348 kb on + strand, within pin at 1209.397 kb on + strand, within pin at 1209.408 kb on + strand at 1209.423 kb on + strand at 1209.612 kb on + strand at 1209.612 kb on + strand at 1209.620 kb on + strand at 1209.635 kb on + strand at 1209.648 kb on + strand at 1209.648 kb on + strand at 1209.663 kb on + strand, within mcrA at 1209.743 kb on + strand, within mcrA at 1209.769 kb on + strand, within mcrA at 1210.309 kb on + strand, within mcrA at 1210.442 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 20 hr rep2_No_BD100_grewON remove 1,207,959 + tfaE b1156 0.36 +1.9 1,207,962 - tfaE b1156 0.37 -1.3 1,207,964 - tfaE b1156 0.37 +0.1 1,207,967 + tfaE b1156 0.38 -1.1 1,207,972 + tfaE b1156 0.38 +0.7 1,208,100 - tfaE b1156 0.60 +0.3 1,208,167 - tfaE b1156 0.71 +0.7 1,208,167 - tfaE b1156 0.71 -0.9 1,208,168 - tfaE b1156 0.71 +0.9 1,208,168 - tfaE b1156 0.71 -0.2 1,208,170 + tfaE b1156 0.71 -1.7 1,208,172 + tfaE b1156 0.72 +0.9 1,208,177 + tfaE b1156 0.72 +0.9 1,208,178 - tfaE b1156 0.73 -0.1 1,208,178 - tfaE b1156 0.73 +1.3 1,208,180 - tfaE b1156 0.73 +1.6 1,208,180 - tfaE b1156 0.73 +0.9 1,208,184 + tfaE b1156 0.74 +0.7 1,208,185 - tfaE b1156 0.74 +1.1 1,208,187 - tfaE b1156 0.74 +2.0 1,208,194 + tfaE b1156 0.75 +1.9 1,208,194 + tfaE b1156 0.75 +0.9 1,208,194 + tfaE b1156 0.75 +0.9 1,208,352 - -2.7 1,208,413 - stfE b1157 0.14 -1.7 1,208,413 - stfE b1157 0.14 -0.4 1,208,441 - stfE b1157 0.20 +0.9 1,208,560 - stfE b1157 0.44 -0.7 1,208,560 - stfE b1157 0.44 -0.3 1,208,575 - stfE b1157 0.47 -0.1 1,208,576 - stfE b1157 0.47 -0.2 1,208,586 - stfE b1157 0.49 -0.4 1,208,604 - stfE b1157 0.52 -0.5 1,208,604 - stfE b1157 0.52 +0.6 1,208,604 - stfE b1157 0.52 +0.8 1,208,606 - stfE b1157 0.53 +0.4 1,208,609 - stfE b1157 0.53 +0.7 1,208,638 - stfE b1157 0.59 +0.0 1,208,642 - stfE b1157 0.60 +0.7 1,208,642 - stfE b1157 0.60 +0.7 1,208,642 - stfE b1157 0.60 +0.9 1,208,648 + stfE b1157 0.61 -2.7 1,208,752 + stfE b1157 0.82 -0.8 1,208,756 - stfE b1157 0.83 +0.4 1,208,791 - stfE b1157 0.90 -0.7 1,208,819 + -0.1 1,208,838 - +0.2 1,208,899 - -0.3 1,208,946 + +1.8 1,208,946 + +1.1 1,208,964 - pin b1158 0.10 +1.0 1,208,964 - pin b1158 0.10 -1.0 1,208,968 + pin b1158 0.11 +0.4 1,209,015 - pin b1158 0.19 -1.2 1,209,110 - pin b1158 0.36 -0.7 1,209,296 + pin b1158 0.70 -2.1 1,209,296 + pin b1158 0.70 -1.5 1,209,306 + pin b1158 0.72 -1.4 1,209,306 + -0.3 1,209,316 + pin b1158 0.74 -0.5 1,209,316 + pin b1158 0.74 +0.0 1,209,333 + pin b1158 0.77 +0.3 1,209,348 + pin b1158 0.79 -0.9 1,209,397 + pin b1158 0.88 +0.2 1,209,408 + -0.2 1,209,423 + +0.8 1,209,612 + +0.1 1,209,612 + -1.6 1,209,620 + -0.2 1,209,635 + -0.4 1,209,648 + -0.2 1,209,648 + +0.3 1,209,663 + mcrA b1159 0.11 -0.1 1,209,743 + mcrA b1159 0.21 -1.4 1,209,769 + mcrA b1159 0.24 -0.7 1,210,309 + mcrA b1159 0.89 +0.9 1,210,442 - +0.9
Or see this region's nucleotide sequence