Strain Fitness in Escherichia coli BW25113 around b4600

Experiment: 20 hr rep1_No_BD100_grewON

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntydfI and ydfJ are separated by 88 nucleotidesydfJ and ydfK are separated by 786 nucleotides b1542: ydfI - predicted mannonate dehydrogenase (NCBI), at 1,627,477 to 1,628,937 ydfI b4600: ydfJ - no description, at 1,629,026 to 1,630,309 ydfJ b1544: ydfK - cold shock protein, function unknown, Qin prophage (NCBI), at 1,631,096 to 1,631,329 ydfK Position (kb) 1629 1630 1631Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 1628.496 kb on + strand, within ydfIat 1628.633 kb on + strand, within ydfIat 1628.633 kb on + strand, within ydfIat 1628.637 kb on - strand, within ydfIat 1628.678 kb on + strand, within ydfIat 1628.757 kb on + strand, within ydfIat 1628.862 kb on + strandat 1629.011 kb on - strandat 1629.015 kb on - strandat 1629.024 kb on - strandat 1629.024 kb on - strandat 1629.029 kb on - strandat 1629.029 kb on - strandat 1629.049 kb on - strandat 1629.053 kb on + strandat 1629.054 kb on - strandat 1629.054 kb on - strandat 1629.060 kb on - strandat 1629.062 kb on - strandat 1629.091 kb on - strandat 1629.145 kb on - strandat 1629.145 kb on - strandat 1629.302 kb on - strand, within ydfJat 1629.312 kb on - strand, within ydfJat 1629.312 kb on - strand, within ydfJat 1629.312 kb on - strand, within ydfJat 1629.321 kb on - strand, within ydfJat 1629.434 kb on - strand, within ydfJat 1629.434 kb on - strand, within ydfJat 1629.460 kb on - strand, within ydfJat 1629.491 kb on - strand, within ydfJat 1629.583 kb on - strand, within ydfJat 1629.723 kb on + strand, within ydfJat 1629.793 kb on + strand, within ydfJat 1629.885 kb on + strand, within ydfJat 1629.902 kb on + strand, within ydfJat 1630.068 kb on - strand, within ydfJat 1630.070 kb on - strand, within ydfJat 1630.082 kb on + strand, within ydfJat 1630.092 kb on + strand, within ydfJat 1630.112 kb on - strand, within ydfJat 1630.203 kb on - strandat 1630.284 kb on - strandat 1630.284 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 20 hr rep1_No_BD100_grewON
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1,628,496 + ydfI b1542 0.70 -0.6
1,628,633 + ydfI b1542 0.79 -0.6
1,628,633 + ydfI b1542 0.79 -1.5
1,628,637 - ydfI b1542 0.79 -0.4
1,628,678 + ydfI b1542 0.82 +0.1
1,628,757 + ydfI b1542 0.88 -0.9
1,628,862 + +1.0
1,629,011 - +0.5
1,629,015 - +0.7
1,629,024 - +1.0
1,629,024 - -0.1
1,629,029 - +0.2
1,629,029 - +0.1
1,629,049 - +0.6
1,629,053 + +0.6
1,629,054 - +1.8
1,629,054 - -0.6
1,629,060 - +1.7
1,629,062 - -0.3
1,629,091 - -0.5
1,629,145 - +0.1
1,629,145 - +0.5
1,629,302 - ydfJ b4600 0.21 -1.6
1,629,312 - ydfJ b4600 0.22 +0.4
1,629,312 - ydfJ b4600 0.22 -2.9
1,629,312 - ydfJ b4600 0.22 -1.4
1,629,321 - ydfJ b4600 0.23 +0.3
1,629,434 - ydfJ b4600 0.32 -1.0
1,629,434 - ydfJ b4600 0.32 +0.7
1,629,460 - ydfJ b4600 0.34 -0.2
1,629,491 - ydfJ b4600 0.36 -0.2
1,629,583 - ydfJ b4600 0.43 -0.1
1,629,723 + ydfJ b4600 0.54 +0.6
1,629,793 + ydfJ b4600 0.60 +1.6
1,629,885 + ydfJ b4600 0.67 -1.4
1,629,902 + ydfJ b4600 0.68 -1.4
1,630,068 - ydfJ b4600 0.81 +0.6
1,630,070 - ydfJ b4600 0.81 +0.6
1,630,082 + ydfJ b4600 0.82 -0.2
1,630,092 + ydfJ b4600 0.83 +0.6
1,630,112 - ydfJ b4600 0.85 +0.6
1,630,203 - +0.6
1,630,284 - -1.4
1,630,284 - -0.4

Or see this region's nucleotide sequence