Experiment: 20 hr rep1_No_BD100_grewON
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt thiH and thiG overlap by 4 nucleotides thiG and thiS are separated by 1 nucleotides thiS and thiF overlap by 17 nucleotides thiF and thiE overlap by 8 nucleotides
b3990: thiH - thiamine biosynthesis protein ThiH (NCBI), at 4,188,758 to 4,189,891
thiH
b3991: thiG - thiamin biosynthesis, thiazole moiety (VIMSS), at 4,189,888 to 4,190,658
thiG
b4407: thiS - sulfur carrier protein ThiS (NCBI), at 4,190,660 to 4,190,860
thiS
b3992: thiF - thiamin biosynthesis, thiazole moiety (VIMSS), at 4,190,844 to 4,191,599
thiF
b3993: thiE - thiamine-phosphate pyrophosphorylase (NCBI), at 4,191,592 to 4,192,227
thiE
Position (kb)
4190
4191 Strain fitness (log2 ratio)
-2
-1
0
1 at 4189.724 kb on + strand, within thiH at 4189.737 kb on - strand, within thiH at 4189.737 kb on - strand, within thiH at 4189.759 kb on - strand, within thiH at 4189.759 kb on - strand, within thiH at 4190.005 kb on - strand, within thiG at 4190.005 kb on - strand, within thiG at 4190.187 kb on - strand, within thiG at 4190.187 kb on - strand, within thiG at 4190.253 kb on + strand, within thiG at 4190.267 kb on - strand, within thiG at 4190.267 kb on - strand, within thiG at 4190.270 kb on + strand, within thiG at 4190.270 kb on + strand, within thiG at 4190.278 kb on - strand, within thiG at 4190.278 kb on - strand, within thiG at 4190.287 kb on - strand, within thiG at 4190.306 kb on - strand, within thiG at 4190.306 kb on - strand, within thiG at 4190.404 kb on + strand, within thiG at 4190.412 kb on - strand, within thiG at 4190.528 kb on - strand, within thiG at 4190.579 kb on + strand, within thiG at 4190.581 kb on + strand, within thiG at 4190.581 kb on + strand, within thiG at 4190.584 kb on - strand at 4190.586 kb on - strand at 4190.791 kb on + strand, within thiS at 4190.820 kb on + strand, within thiS at 4190.830 kb on + strand, within thiS at 4190.860 kb on - strand at 4190.908 kb on - strand at 4190.968 kb on - strand, within thiF at 4190.968 kb on - strand, within thiF at 4191.030 kb on + strand, within thiF at 4191.121 kb on + strand, within thiF at 4191.121 kb on + strand, within thiF at 4191.129 kb on - strand, within thiF at 4191.234 kb on + strand, within thiF at 4191.236 kb on - strand, within thiF at 4191.248 kb on + strand, within thiF at 4191.269 kb on + strand, within thiF at 4191.282 kb on - strand, within thiF at 4191.310 kb on - strand, within thiF at 4191.368 kb on - strand, within thiF at 4191.376 kb on + strand, within thiF at 4191.468 kb on - strand, within thiF at 4191.475 kb on - strand, within thiF at 4191.475 kb on - strand, within thiF at 4191.483 kb on - strand, within thiF at 4191.483 kb on - strand, within thiF at 4191.489 kb on + strand, within thiF at 4191.489 kb on + strand, within thiF at 4191.489 kb on + strand, within thiF at 4191.489 kb on + strand, within thiF at 4191.680 kb on + strand, within thiE at 4191.690 kb on + strand, within thiE at 4191.731 kb on - strand, within thiE at 4191.755 kb on + strand, within thiE at 4191.755 kb on + strand, within thiE at 4191.859 kb on - strand, within thiE
Per-strain Table
Position Strand Gene LocusTag Fraction 20 hr rep1_No_BD100_grewON remove 4,189,724 + thiH b3990 0.85 +0.0 4,189,737 - thiH b3990 0.86 +0.7 4,189,737 - thiH b3990 0.86 -0.8 4,189,759 - thiH b3990 0.88 -1.1 4,189,759 - thiH b3990 0.88 +0.1 4,190,005 - thiG b3991 0.15 +0.3 4,190,005 - thiG b3991 0.15 -0.1 4,190,187 - thiG b3991 0.39 -0.5 4,190,187 - thiG b3991 0.39 -0.8 4,190,253 + thiG b3991 0.47 +1.0 4,190,267 - thiG b3991 0.49 -1.0 4,190,267 - thiG b3991 0.49 +0.5 4,190,270 + thiG b3991 0.50 +0.8 4,190,270 + thiG b3991 0.50 -0.1 4,190,278 - thiG b3991 0.51 +0.2 4,190,278 - thiG b3991 0.51 -0.1 4,190,287 - thiG b3991 0.52 -0.5 4,190,306 - thiG b3991 0.54 +1.1 4,190,306 - thiG b3991 0.54 -0.2 4,190,404 + thiG b3991 0.67 -1.2 4,190,412 - thiG b3991 0.68 +0.4 4,190,528 - thiG b3991 0.83 +0.3 4,190,579 + thiG b3991 0.90 -0.9 4,190,581 + thiG b3991 0.90 -0.2 4,190,581 + thiG b3991 0.90 +1.0 4,190,584 - +0.0 4,190,586 - +0.4 4,190,791 + thiS b4407 0.65 -1.0 4,190,820 + thiS b4407 0.80 -1.0 4,190,830 + thiS b4407 0.85 -0.3 4,190,860 - -1.6 4,190,908 - -0.2 4,190,968 - thiF b3992 0.16 +0.5 4,190,968 - thiF b3992 0.16 +0.3 4,191,030 + thiF b3992 0.25 +0.2 4,191,121 + thiF b3992 0.37 +0.4 4,191,121 + thiF b3992 0.37 +0.4 4,191,129 - thiF b3992 0.38 -0.5 4,191,234 + thiF b3992 0.52 +0.4 4,191,236 - thiF b3992 0.52 -0.1 4,191,248 + thiF b3992 0.53 +1.1 4,191,269 + thiF b3992 0.56 +0.0 4,191,282 - thiF b3992 0.58 -0.0 4,191,310 - thiF b3992 0.62 +0.8 4,191,368 - thiF b3992 0.69 -0.0 4,191,376 + thiF b3992 0.70 -0.9 4,191,468 - thiF b3992 0.83 +0.3 4,191,475 - thiF b3992 0.83 +0.7 4,191,475 - thiF b3992 0.83 +0.1 4,191,483 - thiF b3992 0.85 +0.6 4,191,483 - thiF b3992 0.85 +0.9 4,191,489 + thiF b3992 0.85 +0.6 4,191,489 + thiF b3992 0.85 -1.8 4,191,489 + thiF b3992 0.85 +0.6 4,191,489 + thiF b3992 0.85 +0.3 4,191,680 + thiE b3993 0.14 +0.5 4,191,690 + thiE b3993 0.15 -0.1 4,191,731 - thiE b3993 0.22 +1.3 4,191,755 + thiE b3993 0.26 +0.4 4,191,755 + thiE b3993 0.26 +0.0 4,191,859 - thiE b3993 0.42 -1.0
Or see this region's nucleotide sequence