Experiment: 20 hr rep1_No_BD100_grewON
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cmtA and cmtB are separated by 27 nucleotides cmtB and tktA are separated by 313 nucleotides tktA and yggG are separated by 277 nucleotides
b2933: cmtA - predicted fused mannitol-specific PTS enzymes: IIB component/IIC component (NCBI), at 3,075,493 to 3,076,881
cmtA
b2934: cmtB - predicted mannitol-specific enzyme IIA component of PTS (NCBI), at 3,076,909 to 3,077,352
cmtB
b2935: tktA - transketolase 1, thiamin-binding (NCBI), at 3,077,666 to 3,079,657
tktA
b2936: yggG - orf, hypothetical protein (VIMSS), at 3,079,935 to 3,080,693
yggG
Position (kb)
3077
3078
3079
3080 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 3076.668 kb on + strand, within cmtA at 3076.668 kb on + strand, within cmtA at 3076.676 kb on + strand, within cmtA at 3076.676 kb on + strand, within cmtA at 3076.676 kb on + strand, within cmtA at 3076.677 kb on + strand, within cmtA at 3076.677 kb on + strand, within cmtA at 3076.701 kb on + strand, within cmtA at 3076.750 kb on + strand at 3076.750 kb on + strand at 3076.754 kb on + strand at 3076.754 kb on + strand at 3076.813 kb on - strand at 3076.837 kb on + strand at 3076.837 kb on - strand at 3076.841 kb on + strand at 3076.853 kb on + strand at 3076.854 kb on + strand at 3076.854 kb on + strand at 3077.141 kb on - strand, within cmtB at 3077.180 kb on + strand, within cmtB at 3077.305 kb on + strand, within cmtB at 3077.305 kb on + strand, within cmtB at 3077.438 kb on - strand at 3077.496 kb on - strand at 3077.496 kb on - strand at 3077.516 kb on + strand at 3077.531 kb on + strand at 3077.531 kb on + strand at 3077.576 kb on + strand at 3077.596 kb on - strand at 3077.596 kb on - strand at 3077.617 kb on - strand at 3077.695 kb on - strand at 3077.707 kb on - strand at 3077.717 kb on - strand at 3077.778 kb on - strand at 3077.778 kb on - strand at 3078.018 kb on + strand, within tktA at 3078.067 kb on + strand, within tktA at 3078.104 kb on + strand, within tktA at 3078.233 kb on + strand, within tktA at 3078.406 kb on - strand, within tktA at 3078.580 kb on + strand, within tktA at 3078.776 kb on + strand, within tktA at 3079.066 kb on - strand, within tktA at 3079.341 kb on - strand, within tktA at 3079.494 kb on + strand at 3079.549 kb on - strand at 3079.762 kb on + strand at 3079.812 kb on - strand at 3079.870 kb on + strand at 3079.977 kb on + strand at 3079.985 kb on - strand at 3080.005 kb on + strand at 3080.126 kb on + strand, within yggG at 3080.177 kb on + strand, within yggG at 3080.197 kb on - strand, within yggG at 3080.220 kb on - strand, within yggG at 3080.220 kb on - strand, within yggG at 3080.246 kb on + strand, within yggG at 3080.275 kb on + strand, within yggG at 3080.302 kb on - strand, within yggG at 3080.340 kb on + strand, within yggG at 3080.348 kb on - strand, within yggG at 3080.540 kb on + strand, within yggG at 3080.553 kb on - strand, within yggG at 3080.626 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 20 hr rep1_No_BD100_grewON remove 3,076,668 + cmtA b2933 0.85 -0.3 3,076,668 + cmtA b2933 0.85 -0.5 3,076,676 + cmtA b2933 0.85 +0.3 3,076,676 + cmtA b2933 0.85 +1.3 3,076,676 + cmtA b2933 0.85 -0.5 3,076,677 + cmtA b2933 0.85 +0.0 3,076,677 + cmtA b2933 0.85 +0.2 3,076,701 + cmtA b2933 0.87 +0.4 3,076,750 + -0.9 3,076,750 + -0.4 3,076,754 + -0.2 3,076,754 + +0.4 3,076,813 - +1.7 3,076,837 + -1.8 3,076,837 - -0.5 3,076,841 + -1.9 3,076,853 + -0.5 3,076,854 + -0.4 3,076,854 + -0.0 3,077,141 - cmtB b2934 0.52 +1.4 3,077,180 + cmtB b2934 0.61 +1.4 3,077,305 + cmtB b2934 0.89 +1.8 3,077,305 + cmtB b2934 0.89 +3.0 3,077,438 - +0.1 3,077,496 - +1.0 3,077,496 - +0.4 3,077,516 + -0.9 3,077,531 + -0.4 3,077,531 + -2.5 3,077,576 + -0.1 3,077,596 - +4.7 3,077,596 - +0.2 3,077,617 - +0.3 3,077,695 - -1.1 3,077,707 - -1.6 3,077,717 - -0.4 3,077,778 - -0.2 3,077,778 - -1.6 3,078,018 + tktA b2935 0.18 -0.9 3,078,067 + tktA b2935 0.20 -0.6 3,078,104 + tktA b2935 0.22 +1.0 3,078,233 + tktA b2935 0.28 +0.8 3,078,406 - tktA b2935 0.37 -0.8 3,078,580 + tktA b2935 0.46 +0.6 3,078,776 + tktA b2935 0.56 +1.4 3,079,066 - tktA b2935 0.70 -0.9 3,079,341 - tktA b2935 0.84 -1.9 3,079,494 + -1.8 3,079,549 - -0.2 3,079,762 + -0.5 3,079,812 - -1.2 3,079,870 + -0.0 3,079,977 + -0.5 3,079,985 - +0.1 3,080,005 + +1.1 3,080,126 + yggG b2936 0.25 +0.3 3,080,177 + yggG b2936 0.32 +1.3 3,080,197 - yggG b2936 0.35 -1.5 3,080,220 - yggG b2936 0.38 +0.4 3,080,220 - yggG b2936 0.38 +0.6 3,080,246 + yggG b2936 0.41 +0.5 3,080,275 + yggG b2936 0.45 -0.1 3,080,302 - yggG b2936 0.48 +0.2 3,080,340 + yggG b2936 0.53 +0.6 3,080,348 - yggG b2936 0.54 +0.9 3,080,540 + yggG b2936 0.80 +0.1 3,080,553 - yggG b2936 0.81 +0.5 3,080,626 - -1.1
Or see this region's nucleotide sequence