Experiment: 20 hr rep1_No_BD100_grewON
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt miaA and hfq are separated by 85 nucleotides hfq and hflX are separated by 75 nucleotides
b4171: miaA - tRNA delta(2)-isopentenylpyrophosphate transferase (NCBI), at 4,397,275 to 4,398,225
miaA
b4172: hfq - RNA-binding protein Hfq (NCBI), at 4,398,311 to 4,398,619
hfq
b4173: hflX - predicted GTPase (NCBI), at 4,398,695 to 4,399,975
hflX
Position (kb)
4398
4399 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 4397.420 kb on + strand, within miaA at 4397.449 kb on + strand, within miaA at 4397.449 kb on + strand, within miaA at 4397.455 kb on + strand, within miaA at 4397.458 kb on - strand, within miaA at 4397.505 kb on + strand, within miaA at 4397.549 kb on - strand, within miaA at 4397.584 kb on + strand, within miaA at 4397.589 kb on - strand, within miaA at 4397.704 kb on - strand, within miaA at 4397.763 kb on - strand, within miaA at 4397.845 kb on - strand, within miaA at 4397.853 kb on + strand, within miaA at 4397.856 kb on + strand, within miaA at 4397.866 kb on + strand, within miaA at 4397.922 kb on + strand, within miaA at 4397.935 kb on + strand, within miaA at 4397.935 kb on + strand, within miaA at 4398.137 kb on + strand at 4398.137 kb on + strand at 4398.156 kb on + strand at 4398.233 kb on + strand at 4398.233 kb on + strand at 4398.433 kb on + strand, within hfq at 4398.562 kb on + strand, within hfq at 4398.565 kb on + strand, within hfq at 4398.593 kb on + strand at 4398.668 kb on - strand at 4398.675 kb on - strand at 4398.677 kb on + strand at 4398.677 kb on + strand at 4398.703 kb on + strand at 4398.714 kb on + strand at 4398.724 kb on - strand at 4398.728 kb on + strand at 4398.728 kb on + strand at 4398.793 kb on + strand at 4398.815 kb on - strand at 4398.815 kb on - strand at 4398.837 kb on + strand, within hflX at 4398.926 kb on - strand, within hflX at 4398.961 kb on + strand, within hflX at 4398.961 kb on + strand, within hflX at 4398.965 kb on + strand, within hflX at 4398.965 kb on + strand, within hflX at 4398.968 kb on - strand, within hflX at 4398.983 kb on - strand, within hflX at 4398.983 kb on - strand, within hflX at 4398.992 kb on - strand, within hflX at 4399.118 kb on + strand, within hflX at 4399.140 kb on + strand, within hflX at 4399.154 kb on + strand, within hflX at 4399.217 kb on - strand, within hflX at 4399.307 kb on + strand, within hflX at 4399.307 kb on + strand, within hflX at 4399.365 kb on - strand, within hflX at 4399.365 kb on - strand, within hflX at 4399.407 kb on + strand, within hflX at 4399.407 kb on + strand, within hflX at 4399.421 kb on - strand, within hflX at 4399.449 kb on - strand, within hflX at 4399.457 kb on - strand, within hflX at 4399.457 kb on - strand, within hflX at 4399.457 kb on - strand, within hflX at 4399.500 kb on - strand, within hflX at 4399.500 kb on - strand, within hflX at 4399.542 kb on + strand, within hflX
Per-strain Table
Position Strand Gene LocusTag Fraction 20 hr rep1_No_BD100_grewON remove 4,397,420 + miaA b4171 0.15 +0.6 4,397,449 + miaA b4171 0.18 +1.0 4,397,449 + miaA b4171 0.18 +0.7 4,397,455 + miaA b4171 0.19 -3.2 4,397,458 - miaA b4171 0.19 -3.2 4,397,505 + miaA b4171 0.24 -0.2 4,397,549 - miaA b4171 0.29 +1.7 4,397,584 + miaA b4171 0.32 +0.6 4,397,589 - miaA b4171 0.33 -1.4 4,397,704 - miaA b4171 0.45 +0.3 4,397,763 - miaA b4171 0.51 -0.3 4,397,845 - miaA b4171 0.60 +0.1 4,397,853 + miaA b4171 0.61 -0.3 4,397,856 + miaA b4171 0.61 -1.4 4,397,866 + miaA b4171 0.62 -2.0 4,397,922 + miaA b4171 0.68 -1.7 4,397,935 + miaA b4171 0.69 +1.3 4,397,935 + miaA b4171 0.69 -0.5 4,398,137 + +0.8 4,398,137 + -1.3 4,398,156 + +0.0 4,398,233 + -3.7 4,398,233 + -1.2 4,398,433 + hfq b4172 0.39 +0.7 4,398,562 + hfq b4172 0.81 -1.6 4,398,565 + hfq b4172 0.82 -0.1 4,398,593 + -0.9 4,398,668 - +0.9 4,398,675 - +1.6 4,398,677 + +0.7 4,398,677 + +1.0 4,398,703 + +0.3 4,398,714 + -1.4 4,398,724 - +0.4 4,398,728 + +0.5 4,398,728 + -0.3 4,398,793 + +0.2 4,398,815 - +0.9 4,398,815 - -0.7 4,398,837 + hflX b4173 0.11 +0.7 4,398,926 - hflX b4173 0.18 -1.1 4,398,961 + hflX b4173 0.21 +0.2 4,398,961 + hflX b4173 0.21 +0.4 4,398,965 + hflX b4173 0.21 -0.3 4,398,965 + hflX b4173 0.21 +0.4 4,398,968 - hflX b4173 0.21 -1.2 4,398,983 - hflX b4173 0.22 -1.4 4,398,983 - hflX b4173 0.22 -1.8 4,398,992 - hflX b4173 0.23 -0.1 4,399,118 + hflX b4173 0.33 +0.2 4,399,140 + hflX b4173 0.35 -0.5 4,399,154 + hflX b4173 0.36 -0.9 4,399,217 - hflX b4173 0.41 -2.3 4,399,307 + hflX b4173 0.48 -2.1 4,399,307 + hflX b4173 0.48 -0.6 4,399,365 - hflX b4173 0.52 -1.2 4,399,365 - hflX b4173 0.52 -0.5 4,399,407 + hflX b4173 0.56 +0.9 4,399,407 + hflX b4173 0.56 -0.3 4,399,421 - hflX b4173 0.57 +0.6 4,399,449 - hflX b4173 0.59 +0.2 4,399,457 - hflX b4173 0.59 +0.6 4,399,457 - hflX b4173 0.59 +0.5 4,399,457 - hflX b4173 0.59 -1.4 4,399,500 - hflX b4173 0.63 +0.3 4,399,500 - hflX b4173 0.63 +0.4 4,399,542 + hflX b4173 0.66 +0.2
Or see this region's nucleotide sequence