Experiment: 20 hr rep1_No_BD100_grewON
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt lepB and lepA are separated by 15 nucleotides lepA and rseC are separated by 197 nucleotides rseC and rseB overlap by 4 nucleotides
b2568: lepB - leader peptidase (signal peptidase I) (NCBI), at 2,702,357 to 2,703,331
lepB
b2569: lepA - GTP-binding protein LepA (NCBI), at 2,703,347 to 2,705,146
lepA
b2570: rseC - RseC protein involved in reduction of the SoxR iron-sulfur cluster (NCBI), at 2,705,344 to 2,705,823
rseC
b2571: rseB - periplasmic negative regulator of sigmaE (NCBI), at 2,705,820 to 2,706,776
rseB
Position (kb)
2703
2704
2705
2706 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2702.368 kb on + strand at 2702.368 kb on + strand at 2702.439 kb on - strand at 2702.448 kb on - strand at 2702.448 kb on - strand at 2703.342 kb on - strand at 2703.342 kb on - strand at 2703.413 kb on - strand at 2703.702 kb on - strand, within lepA at 2703.911 kb on - strand, within lepA at 2703.930 kb on - strand, within lepA at 2703.932 kb on - strand, within lepA at 2704.000 kb on - strand, within lepA at 2704.055 kb on + strand, within lepA at 2704.089 kb on + strand, within lepA at 2704.109 kb on - strand, within lepA at 2704.109 kb on - strand, within lepA at 2704.110 kb on - strand, within lepA at 2704.169 kb on + strand, within lepA at 2704.194 kb on - strand, within lepA at 2704.250 kb on - strand, within lepA at 2704.376 kb on - strand, within lepA at 2704.376 kb on - strand, within lepA at 2704.457 kb on - strand, within lepA at 2704.567 kb on - strand, within lepA at 2704.618 kb on - strand, within lepA at 2704.665 kb on - strand, within lepA at 2704.752 kb on + strand, within lepA at 2704.762 kb on - strand, within lepA at 2704.865 kb on - strand, within lepA at 2704.992 kb on + strand at 2705.007 kb on - strand at 2705.209 kb on - strand at 2705.227 kb on - strand at 2705.227 kb on - strand at 2705.249 kb on - strand at 2705.259 kb on - strand at 2705.268 kb on + strand at 2705.500 kb on + strand, within rseC at 2705.500 kb on + strand, within rseC at 2705.554 kb on - strand, within rseC at 2705.594 kb on + strand, within rseC at 2705.652 kb on + strand, within rseC at 2705.652 kb on + strand, within rseC at 2705.662 kb on + strand, within rseC at 2705.662 kb on + strand, within rseC at 2705.673 kb on + strand, within rseC at 2705.699 kb on - strand, within rseC at 2705.699 kb on - strand, within rseC at 2705.760 kb on + strand, within rseC at 2705.761 kb on - strand, within rseC at 2705.761 kb on - strand, within rseC at 2705.783 kb on - strand at 2705.783 kb on - strand at 2705.814 kb on - strand at 2705.915 kb on + strand at 2705.915 kb on + strand at 2705.923 kb on - strand, within rseB at 2705.987 kb on - strand, within rseB at 2706.013 kb on + strand, within rseB
Per-strain Table
Position Strand Gene LocusTag Fraction 20 hr rep1_No_BD100_grewON remove 2,702,368 + +0.1 2,702,368 + +1.1 2,702,439 - -2.2 2,702,448 - -0.7 2,702,448 - -1.3 2,703,342 - -0.9 2,703,342 - -0.3 2,703,413 - +0.6 2,703,702 - lepA b2569 0.20 -0.0 2,703,911 - lepA b2569 0.31 -0.3 2,703,930 - lepA b2569 0.32 -0.3 2,703,932 - lepA b2569 0.33 -0.7 2,704,000 - lepA b2569 0.36 -1.3 2,704,055 + lepA b2569 0.39 -1.0 2,704,089 + lepA b2569 0.41 -1.0 2,704,109 - lepA b2569 0.42 +1.5 2,704,109 - lepA b2569 0.42 -1.1 2,704,110 - lepA b2569 0.42 -0.7 2,704,169 + lepA b2569 0.46 -2.0 2,704,194 - lepA b2569 0.47 -0.6 2,704,250 - lepA b2569 0.50 -0.1 2,704,376 - lepA b2569 0.57 -1.5 2,704,376 - lepA b2569 0.57 -2.8 2,704,457 - lepA b2569 0.62 -0.4 2,704,567 - lepA b2569 0.68 -0.3 2,704,618 - lepA b2569 0.71 -1.7 2,704,665 - lepA b2569 0.73 -2.1 2,704,752 + lepA b2569 0.78 -0.8 2,704,762 - lepA b2569 0.79 -1.0 2,704,865 - lepA b2569 0.84 +1.3 2,704,992 + -1.0 2,705,007 - -0.6 2,705,209 - +0.2 2,705,227 - +0.3 2,705,227 - +0.8 2,705,249 - +0.7 2,705,259 - -0.4 2,705,268 + -0.3 2,705,500 + rseC b2570 0.33 +0.0 2,705,500 + rseC b2570 0.33 +0.2 2,705,554 - rseC b2570 0.44 +1.0 2,705,594 + rseC b2570 0.52 +0.1 2,705,652 + rseC b2570 0.64 +0.0 2,705,652 + rseC b2570 0.64 +0.3 2,705,662 + rseC b2570 0.66 +0.1 2,705,662 + rseC b2570 0.66 -0.3 2,705,673 + rseC b2570 0.69 -0.7 2,705,699 - rseC b2570 0.74 +0.2 2,705,699 - rseC b2570 0.74 -1.1 2,705,760 + rseC b2570 0.87 +0.3 2,705,761 - rseC b2570 0.87 +0.3 2,705,761 - rseC b2570 0.87 -1.4 2,705,783 - -0.0 2,705,783 - -1.4 2,705,814 - +0.2 2,705,915 + +0.7 2,705,915 + +1.0 2,705,923 - rseB b2571 0.11 -0.2 2,705,987 - rseB b2571 0.17 +0.2 2,706,013 + rseB b2571 0.20 +0.1
Or see this region's nucleotide sequence