Experiment: 20 hr rep1_No_BD100_grewON
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt aroC and yfcB are separated by 34 nucleotides yfcB and yfcN are separated by 165 nucleotides yfcN and b2332 are separated by 70 nucleotides
b2329: aroC - chorismate synthase (NCBI), at 2,444,410 to 2,445,495
aroC
b2330: yfcB - putative adenine-specific methylase (VIMSS), at 2,445,530 to 2,446,462
yfcB
b2331: yfcN - hypothetical protein (NCBI), at 2,446,628 to 2,447,179
yfcN
b2332: b2332 - orf, hypothetical protein (VIMSS), at 2,447,250 to 2,448,071
b2332
Position (kb)
2445
2446
2447 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2444.552 kb on - strand, within aroC at 2444.844 kb on + strand, within aroC at 2444.866 kb on - strand, within aroC at 2444.923 kb on - strand, within aroC at 2444.997 kb on - strand, within aroC at 2444.997 kb on - strand, within aroC at 2445.075 kb on + strand, within aroC at 2445.075 kb on + strand, within aroC at 2445.180 kb on + strand, within aroC at 2445.213 kb on + strand, within aroC at 2445.213 kb on + strand, within aroC at 2445.219 kb on - strand, within aroC at 2445.219 kb on - strand, within aroC at 2445.244 kb on + strand, within aroC at 2445.326 kb on + strand, within aroC at 2445.326 kb on + strand, within aroC at 2445.337 kb on - strand, within aroC at 2445.337 kb on - strand, within aroC at 2445.487 kb on - strand at 2445.492 kb on - strand at 2445.514 kb on - strand at 2445.514 kb on - strand at 2445.577 kb on + strand at 2445.660 kb on - strand, within yfcB at 2445.661 kb on + strand, within yfcB at 2445.661 kb on + strand, within yfcB at 2445.767 kb on - strand, within yfcB at 2446.057 kb on - strand, within yfcB at 2446.131 kb on + strand, within yfcB at 2446.369 kb on - strand at 2446.653 kb on - strand at 2446.706 kb on - strand, within yfcN at 2446.706 kb on - strand, within yfcN at 2446.713 kb on - strand, within yfcN at 2446.948 kb on - strand, within yfcN at 2446.955 kb on + strand, within yfcN at 2446.963 kb on - strand, within yfcN at 2446.963 kb on - strand, within yfcN at 2447.022 kb on + strand, within yfcN at 2447.073 kb on - strand, within yfcN at 2447.073 kb on - strand, within yfcN at 2447.202 kb on + strand at 2447.202 kb on + strand at 2447.217 kb on + strand at 2447.260 kb on - strand at 2447.305 kb on + strand at 2447.305 kb on - strand at 2447.305 kb on - strand at 2447.334 kb on + strand, within b2332 at 2447.350 kb on + strand, within b2332 at 2447.428 kb on + strand, within b2332 at 2447.428 kb on + strand, within b2332
Per-strain Table
Position Strand Gene LocusTag Fraction 20 hr rep1_No_BD100_grewON remove 2,444,552 - aroC b2329 0.13 -0.5 2,444,844 + aroC b2329 0.40 +0.4 2,444,866 - aroC b2329 0.42 -0.3 2,444,923 - aroC b2329 0.47 +0.3 2,444,997 - aroC b2329 0.54 +1.5 2,444,997 - aroC b2329 0.54 -3.0 2,445,075 + aroC b2329 0.61 +0.2 2,445,075 + aroC b2329 0.61 +0.6 2,445,180 + aroC b2329 0.71 +0.7 2,445,213 + aroC b2329 0.74 -1.0 2,445,213 + aroC b2329 0.74 -0.7 2,445,219 - aroC b2329 0.74 -1.9 2,445,219 - aroC b2329 0.74 -0.3 2,445,244 + aroC b2329 0.77 -0.0 2,445,326 + aroC b2329 0.84 -0.6 2,445,326 + aroC b2329 0.84 -2.6 2,445,337 - aroC b2329 0.85 -0.3 2,445,337 - aroC b2329 0.85 +1.8 2,445,487 - -1.9 2,445,492 - -0.2 2,445,514 - -0.8 2,445,514 - -1.7 2,445,577 + -0.5 2,445,660 - yfcB b2330 0.14 -2.0 2,445,661 + yfcB b2330 0.14 +1.7 2,445,661 + yfcB b2330 0.14 -0.5 2,445,767 - yfcB b2330 0.25 -1.5 2,446,057 - yfcB b2330 0.56 -2.0 2,446,131 + yfcB b2330 0.64 -0.5 2,446,369 - -3.1 2,446,653 - -1.2 2,446,706 - yfcN b2331 0.14 -0.6 2,446,706 - yfcN b2331 0.14 -1.4 2,446,713 - yfcN b2331 0.15 +1.7 2,446,948 - yfcN b2331 0.58 +0.6 2,446,955 + yfcN b2331 0.59 +0.4 2,446,963 - yfcN b2331 0.61 -0.6 2,446,963 - yfcN b2331 0.61 -0.3 2,447,022 + yfcN b2331 0.71 +0.2 2,447,073 - yfcN b2331 0.81 -1.1 2,447,073 - yfcN b2331 0.81 -0.3 2,447,202 + -0.1 2,447,202 + +0.8 2,447,217 + +0.5 2,447,260 - -0.3 2,447,305 + +0.6 2,447,305 - -0.7 2,447,305 - -0.9 2,447,334 + b2332 b2332 0.10 +0.1 2,447,350 + b2332 b2332 0.12 +0.3 2,447,428 + b2332 b2332 0.22 -0.2 2,447,428 + b2332 b2332 0.22 +0.0
Or see this region's nucleotide sequence