Experiment: 20 hr rep1_No_BD100_grewON
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt napA and napD overlap by 4 nucleotides napD and napF overlap by 11 nucleotides napF and yojO are separated by 108 nucleotides yojO and eco are separated by 134 nucleotides
b2206: napA - nitrate reductase, periplasmic, large subunit (NCBI), at 2,298,289 to 2,300,775
napA
b2207: napD - assembly protein for periplasmic nitrate reductase (NCBI), at 2,300,772 to 2,301,035
napD
b2208: napF - ferredoxin-type protein, predicted role in electron transfer to periplasmic nitrate reductase (NapA) (NCBI), at 2,301,025 to 2,301,519
napF
b4604: yojO - hypothetical protein (RefSeq), at 2,301,628 to 2,301,792
yojO
b2209: eco - ecotin precursor (NCBI), at 2,301,927 to 2,302,415
eco
Position (kb)
2300
2301
2302 Strain fitness (log2 ratio)
-1
0
1 at 2299.817 kb on - strand, within napA at 2299.859 kb on + strand, within napA at 2300.072 kb on + strand, within napA at 2300.072 kb on + strand, within napA at 2300.114 kb on + strand, within napA at 2300.239 kb on + strand, within napA at 2300.240 kb on - strand, within napA at 2300.245 kb on - strand, within napA at 2300.338 kb on + strand, within napA at 2300.365 kb on + strand, within napA at 2300.365 kb on + strand, within napA at 2300.421 kb on - strand, within napA at 2300.421 kb on - strand, within napA at 2300.605 kb on + strand at 2300.832 kb on + strand, within napD at 2300.841 kb on + strand, within napD at 2300.865 kb on - strand, within napD at 2300.965 kb on + strand, within napD at 2301.037 kb on + strand at 2301.037 kb on + strand at 2301.060 kb on + strand at 2301.060 kb on + strand at 2301.084 kb on + strand, within napF at 2301.183 kb on - strand, within napF at 2301.210 kb on - strand, within napF at 2301.210 kb on - strand, within napF at 2301.256 kb on - strand, within napF at 2301.257 kb on - strand, within napF at 2301.286 kb on + strand, within napF at 2301.415 kb on - strand, within napF at 2301.496 kb on - strand at 2301.620 kb on + strand at 2301.874 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 20 hr rep1_No_BD100_grewON remove 2,299,817 - napA b2206 0.61 -0.2 2,299,859 + napA b2206 0.63 +1.0 2,300,072 + napA b2206 0.72 -0.1 2,300,072 + napA b2206 0.72 -0.2 2,300,114 + napA b2206 0.73 -0.4 2,300,239 + napA b2206 0.78 -0.3 2,300,240 - napA b2206 0.78 +0.1 2,300,245 - napA b2206 0.79 -1.4 2,300,338 + napA b2206 0.82 -0.4 2,300,365 + napA b2206 0.83 +0.6 2,300,365 + napA b2206 0.83 -0.4 2,300,421 - napA b2206 0.86 +0.8 2,300,421 - napA b2206 0.86 +0.9 2,300,605 + -0.6 2,300,832 + napD b2207 0.23 -0.7 2,300,841 + napD b2207 0.26 -0.8 2,300,865 - napD b2207 0.35 -1.7 2,300,965 + napD b2207 0.73 +0.4 2,301,037 + +1.1 2,301,037 + +0.0 2,301,060 + -0.3 2,301,060 + -0.5 2,301,084 + napF b2208 0.12 -1.3 2,301,183 - napF b2208 0.32 +0.1 2,301,210 - napF b2208 0.37 -0.9 2,301,210 - napF b2208 0.37 +0.1 2,301,256 - napF b2208 0.47 -0.7 2,301,257 - napF b2208 0.47 +0.2 2,301,286 + napF b2208 0.53 -0.5 2,301,415 - napF b2208 0.79 +0.0 2,301,496 - +0.1 2,301,620 + +1.0 2,301,874 + -0.5
Or see this region's nucleotide sequence