Experiment: 20 hr rep1_No_BD100_grewON
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt mglA and mglB are separated by 60 nucleotides mglB and galS are separated by 279 nucleotides
b2149: mglA - fused methyl-galactoside transporter subunits of ABC superfamily: ATP-binding components (NCBI), at 2,235,791 to 2,237,311
mglA
b2150: mglB - methyl-galactoside transporter subunit (NCBI), at 2,237,372 to 2,238,370
mglB
b2151: galS - DNA-binding transcriptional repressor (NCBI), at 2,238,650 to 2,239,690
galS
Position (kb)
2237
2238
2239 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2236.387 kb on - strand, within mglA at 2236.421 kb on - strand, within mglA at 2236.446 kb on + strand, within mglA at 2236.466 kb on - strand, within mglA at 2236.478 kb on - strand, within mglA at 2236.510 kb on - strand, within mglA at 2236.510 kb on - strand, within mglA at 2236.559 kb on + strand, within mglA at 2236.579 kb on - strand, within mglA at 2236.579 kb on - strand, within mglA at 2236.626 kb on - strand, within mglA at 2236.697 kb on + strand, within mglA at 2236.697 kb on + strand, within mglA at 2236.895 kb on + strand, within mglA at 2236.901 kb on - strand, within mglA at 2236.943 kb on - strand, within mglA at 2236.947 kb on + strand, within mglA at 2236.959 kb on + strand, within mglA at 2236.962 kb on - strand, within mglA at 2236.962 kb on - strand, within mglA at 2236.988 kb on + strand, within mglA at 2236.988 kb on - strand, within mglA at 2237.034 kb on + strand, within mglA at 2237.079 kb on - strand, within mglA at 2237.084 kb on + strand, within mglA at 2237.193 kb on + strand at 2237.238 kb on - strand at 2237.249 kb on - strand at 2237.267 kb on - strand at 2237.291 kb on - strand at 2237.296 kb on - strand at 2237.296 kb on - strand at 2237.336 kb on - strand at 2237.356 kb on - strand at 2237.356 kb on - strand at 2237.356 kb on - strand at 2237.356 kb on - strand at 2237.407 kb on - strand at 2237.407 kb on - strand at 2237.422 kb on - strand at 2237.422 kb on - strand at 2237.430 kb on - strand at 2237.530 kb on - strand, within mglB at 2237.897 kb on - strand, within mglB at 2237.901 kb on + strand, within mglB at 2237.927 kb on - strand, within mglB at 2237.989 kb on - strand, within mglB at 2238.223 kb on - strand, within mglB at 2238.269 kb on + strand, within mglB at 2238.309 kb on + strand at 2238.309 kb on + strand at 2238.488 kb on + strand at 2238.488 kb on + strand at 2238.602 kb on + strand at 2238.602 kb on + strand at 2238.647 kb on + strand at 2238.762 kb on - strand, within galS at 2238.762 kb on - strand, within galS at 2238.839 kb on - strand, within galS at 2238.857 kb on - strand, within galS at 2239.012 kb on + strand, within galS at 2239.042 kb on - strand, within galS at 2239.066 kb on + strand, within galS at 2239.098 kb on - strand, within galS at 2239.098 kb on - strand, within galS at 2239.112 kb on - strand, within galS at 2239.267 kb on - strand, within galS
Per-strain Table
Position Strand Gene LocusTag Fraction 20 hr rep1_No_BD100_grewON remove 2,236,387 - mglA b2149 0.39 -1.2 2,236,421 - mglA b2149 0.41 +1.0 2,236,446 + mglA b2149 0.43 +1.0 2,236,466 - mglA b2149 0.44 +0.6 2,236,478 - mglA b2149 0.45 +0.2 2,236,510 - mglA b2149 0.47 -2.5 2,236,510 - mglA b2149 0.47 -1.6 2,236,559 + mglA b2149 0.50 +0.0 2,236,579 - mglA b2149 0.52 -0.7 2,236,579 - mglA b2149 0.52 +0.5 2,236,626 - mglA b2149 0.55 +0.2 2,236,697 + mglA b2149 0.60 -0.0 2,236,697 + mglA b2149 0.60 +1.0 2,236,895 + mglA b2149 0.73 +1.0 2,236,901 - mglA b2149 0.73 +0.4 2,236,943 - mglA b2149 0.76 +0.3 2,236,947 + mglA b2149 0.76 +2.5 2,236,959 + mglA b2149 0.77 +0.2 2,236,962 - mglA b2149 0.77 -0.0 2,236,962 - mglA b2149 0.77 +0.4 2,236,988 + mglA b2149 0.79 -0.0 2,236,988 - mglA b2149 0.79 -0.2 2,237,034 + mglA b2149 0.82 +1.8 2,237,079 - mglA b2149 0.85 +0.8 2,237,084 + mglA b2149 0.85 +1.5 2,237,193 + -1.4 2,237,238 - +1.2 2,237,249 - +0.8 2,237,267 - +0.4 2,237,291 - -0.7 2,237,296 - +0.4 2,237,296 - -0.0 2,237,336 - +0.1 2,237,356 - -0.5 2,237,356 - -0.3 2,237,356 - +0.5 2,237,356 - -1.0 2,237,407 - +0.1 2,237,407 - -0.1 2,237,422 - +0.4 2,237,422 - +0.4 2,237,430 - -0.3 2,237,530 - mglB b2150 0.16 -1.0 2,237,897 - mglB b2150 0.53 -1.6 2,237,901 + mglB b2150 0.53 +0.3 2,237,927 - mglB b2150 0.56 -0.6 2,237,989 - mglB b2150 0.62 -0.2 2,238,223 - mglB b2150 0.85 -2.1 2,238,269 + mglB b2150 0.90 -0.0 2,238,309 + +0.8 2,238,309 + +0.7 2,238,488 + +0.6 2,238,488 + -0.2 2,238,602 + +1.0 2,238,602 + +2.1 2,238,647 + +0.4 2,238,762 - galS b2151 0.11 +0.3 2,238,762 - galS b2151 0.11 +0.1 2,238,839 - galS b2151 0.18 +0.5 2,238,857 - galS b2151 0.20 -0.8 2,239,012 + galS b2151 0.35 +0.7 2,239,042 - galS b2151 0.38 +1.0 2,239,066 + galS b2151 0.40 -1.5 2,239,098 - galS b2151 0.43 +0.7 2,239,098 - galS b2151 0.43 +0.1 2,239,112 - galS b2151 0.44 -1.5 2,239,267 - galS b2151 0.59 -2.0
Or see this region's nucleotide sequence