Experiment: 20 hr rep1_No_BD100_grewON
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt wcaC and wcaB overlap by 4 nucleotides wcaB and wcaA are separated by 2 nucleotides wcaA and b2060 are separated by 92 nucleotides
b2057: wcaC - predicted glycosyl transferase (NCBI), at 2,128,877 to 2,130,094
wcaC
b2058: wcaB - predicted acyl transferase (NCBI), at 2,130,091 to 2,130,579
wcaB
b2059: wcaA - predicted glycosyl transferase (NCBI), at 2,130,582 to 2,131,421
wcaA
b2060: b2060 - orf, hypothetical protein (VIMSS), at 2,131,514 to 2,133,676
b2060
Position (kb)
2130
2131 Strain fitness (log2 ratio)
-1
0
1 at 2129.249 kb on - strand, within wcaC at 2129.249 kb on - strand, within wcaC at 2129.459 kb on + strand, within wcaC at 2129.459 kb on + strand, within wcaC at 2129.468 kb on + strand, within wcaC at 2129.473 kb on + strand, within wcaC at 2129.586 kb on + strand, within wcaC at 2129.690 kb on + strand, within wcaC at 2129.798 kb on + strand, within wcaC at 2129.803 kb on + strand, within wcaC at 2130.018 kb on - strand at 2130.025 kb on + strand at 2130.025 kb on + strand at 2130.046 kb on - strand at 2130.159 kb on + strand, within wcaB at 2130.625 kb on + strand at 2130.691 kb on - strand, within wcaA at 2130.691 kb on - strand, within wcaA at 2130.692 kb on - strand, within wcaA at 2130.813 kb on + strand, within wcaA at 2130.841 kb on + strand, within wcaA at 2130.889 kb on - strand, within wcaA at 2131.196 kb on - strand, within wcaA at 2131.212 kb on + strand, within wcaA at 2131.306 kb on - strand, within wcaA at 2131.332 kb on - strand, within wcaA at 2131.395 kb on + strand at 2131.395 kb on + strand at 2131.421 kb on - strand at 2131.519 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 20 hr rep1_No_BD100_grewON remove 2,129,249 - wcaC b2057 0.31 +0.4 2,129,249 - wcaC b2057 0.31 -1.7 2,129,459 + wcaC b2057 0.48 +0.3 2,129,459 + wcaC b2057 0.48 +0.3 2,129,468 + wcaC b2057 0.49 -0.1 2,129,473 + wcaC b2057 0.49 -0.1 2,129,586 + wcaC b2057 0.58 +0.2 2,129,690 + wcaC b2057 0.67 +0.5 2,129,798 + wcaC b2057 0.76 +0.4 2,129,803 + wcaC b2057 0.76 +0.5 2,130,018 - -1.4 2,130,025 + -1.1 2,130,025 + +0.1 2,130,046 - -0.3 2,130,159 + wcaB b2058 0.14 -0.9 2,130,625 + +0.6 2,130,691 - wcaA b2059 0.13 +0.8 2,130,691 - wcaA b2059 0.13 +0.3 2,130,692 - wcaA b2059 0.13 -0.0 2,130,813 + wcaA b2059 0.28 -0.1 2,130,841 + wcaA b2059 0.31 +0.8 2,130,889 - wcaA b2059 0.37 -0.1 2,131,196 - wcaA b2059 0.73 +0.8 2,131,212 + wcaA b2059 0.75 +0.5 2,131,306 - wcaA b2059 0.86 -0.1 2,131,332 - wcaA b2059 0.89 +0.6 2,131,395 + -0.2 2,131,395 + -0.5 2,131,421 - +0.2 2,131,519 - +0.7
Or see this region's nucleotide sequence