Experiment: 20 hr rep1_No_BD100_grewON
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yeeZ and yoeB are separated by 82 nucleotides yoeB and yefM overlap by 4 nucleotides yefM and hisL are separated by 282 nucleotides hisL and hisG are separated by 145 nucleotides
b2016: yeeZ - predicted epimerase, with NAD(P)-binding Rossmann-fold domain (NCBI), at 2,086,328 to 2,087,152
yeeZ
b4539: yoeB - toxin of the YoeB-YefM toxin-antitoxin system (NCBI), at 2,087,235 to 2,087,489
yoeB
b2017: yefM - orf, hypothetical protein (VIMSS), at 2,087,486 to 2,087,737
yefM
b2018: hisL - his operon leader peptide (NCBI), at 2,088,020 to 2,088,070
hisL
b2019: hisG - ATP phosphoribosyltransferase (NCBI), at 2,088,216 to 2,089,115
hisG
Position (kb)
2087
2088 Strain fitness (log2 ratio)
-1
0
1 at 2086.500 kb on + strand, within yeeZ at 2086.556 kb on - strand, within yeeZ at 2086.579 kb on + strand, within yeeZ at 2086.660 kb on + strand, within yeeZ at 2086.660 kb on + strand, within yeeZ at 2086.669 kb on + strand, within yeeZ at 2086.672 kb on - strand, within yeeZ at 2086.672 kb on - strand, within yeeZ at 2086.871 kb on - strand, within yeeZ at 2086.947 kb on - strand, within yeeZ at 2087.058 kb on + strand, within yeeZ at 2087.122 kb on + strand at 2087.178 kb on - strand at 2087.178 kb on - strand at 2087.187 kb on - strand at 2087.197 kb on - strand at 2087.364 kb on - strand, within yoeB at 2087.506 kb on - strand at 2087.506 kb on - strand at 2087.544 kb on + strand, within yefM at 2087.642 kb on - strand, within yefM at 2087.867 kb on + strand at 2088.113 kb on - strand at 2088.203 kb on - strand at 2088.203 kb on - strand at 2088.316 kb on + strand, within hisG at 2088.484 kb on - strand, within hisG at 2088.602 kb on + strand, within hisG at 2088.610 kb on - strand, within hisG at 2088.610 kb on - strand, within hisG at 2088.657 kb on - strand, within hisG at 2088.681 kb on - strand, within hisG
Per-strain Table
Position Strand Gene LocusTag Fraction 20 hr rep1_No_BD100_grewON remove 2,086,500 + yeeZ b2016 0.21 +0.3 2,086,556 - yeeZ b2016 0.28 +0.3 2,086,579 + yeeZ b2016 0.30 +0.4 2,086,660 + yeeZ b2016 0.40 +0.7 2,086,660 + yeeZ b2016 0.40 +0.8 2,086,669 + yeeZ b2016 0.41 -1.1 2,086,672 - yeeZ b2016 0.42 +0.8 2,086,672 - yeeZ b2016 0.42 -0.0 2,086,871 - yeeZ b2016 0.66 +0.6 2,086,947 - yeeZ b2016 0.75 -0.9 2,087,058 + yeeZ b2016 0.88 -1.1 2,087,122 + +0.5 2,087,178 - -0.7 2,087,178 - +0.5 2,087,187 - -0.5 2,087,197 - -0.2 2,087,364 - yoeB b4539 0.51 +0.4 2,087,506 - +0.5 2,087,506 - +1.6 2,087,544 + yefM b2017 0.23 +0.1 2,087,642 - yefM b2017 0.62 -1.5 2,087,867 + -0.7 2,088,113 - -0.4 2,088,203 - -0.1 2,088,203 - +0.4 2,088,316 + hisG b2019 0.11 +0.4 2,088,484 - hisG b2019 0.30 -0.0 2,088,602 + hisG b2019 0.43 +0.1 2,088,610 - hisG b2019 0.44 +0.2 2,088,610 - hisG b2019 0.44 +0.2 2,088,657 - hisG b2019 0.49 +1.5 2,088,681 - hisG b2019 0.52 -0.8
Or see this region's nucleotide sequence