Experiment: 20 hr rep1_No_BD100_grewON
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ydhZ and pykF are separated by 556 nucleotides pykF and lpp are separated by 310 nucleotides lpp and ynhG are separated by 63 nucleotides
b1675: ydhZ - hypothetical protein (NCBI), at 1,752,956 to 1,753,165
ydhZ
b1676: pykF - pyruvate kinase (NCBI), at 1,753,722 to 1,755,134
pykF
b1677: lpp - murein lipoprotein (NCBI), at 1,755,445 to 1,755,681
lpp
b1678: ynhG - hypothetical protein (NCBI), at 1,755,745 to 1,756,749
ynhG
Position (kb)
1753
1754
1755
1756 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1752.920 kb on - strand at 1752.934 kb on - strand at 1753.041 kb on - strand, within ydhZ at 1753.125 kb on - strand, within ydhZ at 1753.126 kb on - strand, within ydhZ at 1753.141 kb on + strand, within ydhZ at 1753.300 kb on - strand at 1753.300 kb on - strand at 1753.313 kb on + strand at 1753.334 kb on + strand at 1753.334 kb on + strand at 1753.334 kb on + strand at 1753.342 kb on - strand at 1753.342 kb on - strand at 1753.342 kb on - strand at 1753.358 kb on + strand at 1753.500 kb on - strand at 1753.500 kb on - strand at 1753.563 kb on + strand at 1753.582 kb on + strand at 1753.615 kb on + strand at 1753.635 kb on - strand at 1753.696 kb on + strand at 1753.710 kb on - strand at 1753.710 kb on - strand at 1753.836 kb on - strand at 1753.836 kb on - strand at 1753.921 kb on + strand, within pykF at 1753.983 kb on + strand, within pykF at 1754.072 kb on + strand, within pykF at 1754.121 kb on - strand, within pykF at 1754.121 kb on - strand, within pykF at 1754.319 kb on - strand, within pykF at 1754.319 kb on - strand, within pykF at 1754.319 kb on - strand, within pykF at 1754.581 kb on + strand, within pykF at 1754.581 kb on + strand, within pykF at 1754.992 kb on + strand, within pykF at 1755.104 kb on + strand at 1755.375 kb on + strand at 1755.410 kb on + strand at 1755.417 kb on + strand at 1755.476 kb on - strand, within lpp at 1755.478 kb on + strand, within lpp at 1755.485 kb on + strand, within lpp at 1755.576 kb on + strand, within lpp at 1755.662 kb on + strand at 1755.782 kb on - strand at 1755.985 kb on - strand, within ynhG at 1755.985 kb on - strand, within ynhG at 1756.008 kb on + strand, within ynhG at 1756.008 kb on + strand, within ynhG at 1756.016 kb on + strand, within ynhG at 1756.016 kb on - strand, within ynhG at 1756.016 kb on - strand, within ynhG at 1756.080 kb on + strand, within ynhG at 1756.080 kb on + strand, within ynhG
Per-strain Table
Position Strand Gene LocusTag Fraction 20 hr rep1_No_BD100_grewON remove 1,752,920 - +0.2 1,752,934 - -0.1 1,753,041 - ydhZ b1675 0.40 +0.1 1,753,125 - ydhZ b1675 0.80 -0.7 1,753,126 - ydhZ b1675 0.81 +0.4 1,753,141 + ydhZ b1675 0.88 +0.7 1,753,300 - +0.4 1,753,300 - -0.3 1,753,313 + -0.3 1,753,334 + +2.2 1,753,334 + +0.3 1,753,334 + -0.9 1,753,342 - +0.4 1,753,342 - +0.0 1,753,342 - +0.1 1,753,358 + +0.6 1,753,500 - -1.9 1,753,500 - -0.0 1,753,563 + -1.3 1,753,582 + +0.2 1,753,615 + +0.2 1,753,635 - -1.2 1,753,696 + -0.5 1,753,710 - -1.0 1,753,710 - -0.5 1,753,836 - -1.5 1,753,836 - -0.7 1,753,921 + pykF b1676 0.14 -2.3 1,753,983 + pykF b1676 0.18 -0.9 1,754,072 + pykF b1676 0.25 -2.4 1,754,121 - pykF b1676 0.28 +0.0 1,754,121 - pykF b1676 0.28 -0.1 1,754,319 - pykF b1676 0.42 -1.9 1,754,319 - pykF b1676 0.42 -2.3 1,754,319 - pykF b1676 0.42 -2.8 1,754,581 + pykF b1676 0.61 -0.8 1,754,581 + pykF b1676 0.61 +1.0 1,754,992 + pykF b1676 0.90 -1.9 1,755,104 + -1.5 1,755,375 + +1.0 1,755,410 + +0.0 1,755,417 + -0.3 1,755,476 - lpp b1677 0.13 +0.5 1,755,478 + lpp b1677 0.14 +1.0 1,755,485 + lpp b1677 0.17 -0.1 1,755,576 + lpp b1677 0.55 -0.8 1,755,662 + -0.7 1,755,782 - +0.9 1,755,985 - ynhG b1678 0.24 -0.6 1,755,985 - ynhG b1678 0.24 -1.2 1,756,008 + ynhG b1678 0.26 +0.2 1,756,008 + ynhG b1678 0.26 -1.1 1,756,016 + ynhG b1678 0.27 -1.6 1,756,016 - ynhG b1678 0.27 +0.6 1,756,016 - ynhG b1678 0.27 +0.9 1,756,080 + ynhG b1678 0.33 -0.1 1,756,080 + ynhG b1678 0.33 -0.5
Or see this region's nucleotide sequence