Experiment: 20 hr rep1_No_BD100_grewON
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt mdtK and ydhQ are separated by 40 nucleotides ydhQ and valV are separated by 307 nucleotides valV and valW are separated by 4 nucleotides valW and ydhR are separated by 107 nucleotides
b1663: mdtK - multidrug efflux protein NorM (NCBI), at 1,741,481 to 1,742,854
mdtK
b1664: ydhQ - hypothetical protein (NCBI), at 1,742,895 to 1,744,151
ydhQ
b1665: valV - tRNA-Val (NCBI), at 1,744,459 to 1,744,535
valV
b1666: valW - tRNA-Val (NCBI), at 1,744,540 to 1,744,616
valW
b1667: ydhR - hypothetical protein (NCBI), at 1,744,724 to 1,745,029
ydhR
Position (kb)
1742
1743
1744
1745 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1741.928 kb on - strand, within mdtK at 1742.088 kb on - strand, within mdtK at 1742.235 kb on + strand, within mdtK at 1742.237 kb on + strand, within mdtK at 1742.253 kb on - strand, within mdtK at 1742.299 kb on + strand, within mdtK at 1742.419 kb on - strand, within mdtK at 1742.436 kb on - strand, within mdtK at 1742.514 kb on - strand, within mdtK at 1742.528 kb on + strand, within mdtK at 1742.696 kb on - strand, within mdtK at 1742.696 kb on - strand, within mdtK at 1742.902 kb on + strand at 1742.950 kb on + strand at 1743.088 kb on + strand, within ydhQ at 1743.088 kb on + strand, within ydhQ at 1743.220 kb on + strand, within ydhQ at 1743.220 kb on + strand, within ydhQ at 1743.225 kb on + strand, within ydhQ at 1743.230 kb on + strand, within ydhQ at 1743.233 kb on - strand, within ydhQ at 1743.380 kb on + strand, within ydhQ at 1743.398 kb on - strand, within ydhQ at 1743.446 kb on + strand, within ydhQ at 1743.446 kb on + strand, within ydhQ at 1743.596 kb on - strand, within ydhQ at 1743.597 kb on - strand, within ydhQ at 1743.597 kb on - strand, within ydhQ at 1743.666 kb on - strand, within ydhQ at 1743.892 kb on + strand, within ydhQ at 1744.114 kb on + strand at 1744.176 kb on - strand at 1744.194 kb on - strand at 1744.570 kb on + strand, within valW at 1744.671 kb on - strand at 1744.797 kb on - strand, within ydhR at 1744.953 kb on + strand, within ydhR
Per-strain Table
Position Strand Gene LocusTag Fraction 20 hr rep1_No_BD100_grewON remove 1,741,928 - mdtK b1663 0.33 +0.6 1,742,088 - mdtK b1663 0.44 +0.6 1,742,235 + mdtK b1663 0.55 +0.4 1,742,237 + mdtK b1663 0.55 +1.4 1,742,253 - mdtK b1663 0.56 +0.6 1,742,299 + mdtK b1663 0.60 +0.6 1,742,419 - mdtK b1663 0.68 -0.4 1,742,436 - mdtK b1663 0.70 -0.0 1,742,514 - mdtK b1663 0.75 -1.4 1,742,528 + mdtK b1663 0.76 +0.1 1,742,696 - mdtK b1663 0.88 -0.9 1,742,696 - mdtK b1663 0.88 +0.7 1,742,902 + -0.2 1,742,950 + -0.1 1,743,088 + ydhQ b1664 0.15 -1.3 1,743,088 + ydhQ b1664 0.15 -1.3 1,743,220 + ydhQ b1664 0.26 -2.9 1,743,220 + ydhQ b1664 0.26 -2.7 1,743,225 + ydhQ b1664 0.26 +0.9 1,743,230 + ydhQ b1664 0.27 -0.7 1,743,233 - ydhQ b1664 0.27 -1.3 1,743,380 + ydhQ b1664 0.39 +0.3 1,743,398 - ydhQ b1664 0.40 -0.9 1,743,446 + ydhQ b1664 0.44 +0.1 1,743,446 + ydhQ b1664 0.44 -3.3 1,743,596 - ydhQ b1664 0.56 +0.3 1,743,597 - ydhQ b1664 0.56 -0.4 1,743,597 - ydhQ b1664 0.56 +0.6 1,743,666 - ydhQ b1664 0.61 -1.9 1,743,892 + ydhQ b1664 0.79 -0.3 1,744,114 + +0.5 1,744,176 - +0.0 1,744,194 - -0.0 1,744,570 + valW b1666 0.39 +0.3 1,744,671 - +0.7 1,744,797 - ydhR b1667 0.24 +0.3 1,744,953 + ydhR b1667 0.75 +0.1
Or see this region's nucleotide sequence