Experiment: 20 hr rep1_No_BD100_grewON
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt sdhB and sucA are separated by 300 nucleotides sucA and sucB are separated by 14 nucleotides
b0724: sdhB - succinate dehydrogenase, FeS subunit (NCBI), at 756,912 to 757,628
sdhB
b0726: sucA - alpha-ketoglutarate decarboxylase (NCBI), at 757,929 to 760,730
sucA
b0727: sucB - dihydrolipoamide acetyltransferase (NCBI), at 760,745 to 761,962
sucB
Position (kb) 757
758
759
760
761 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 757.053 kb on + strand, within sdhB at 757.138 kb on - strand, within sdhB at 757.143 kb on - strand, within sdhB at 757.184 kb on - strand, within sdhB at 757.198 kb on + strand, within sdhB at 757.198 kb on + strand, within sdhB at 757.229 kb on - strand, within sdhB at 757.233 kb on + strand, within sdhB at 757.241 kb on - strand, within sdhB at 757.381 kb on - strand, within sdhB at 757.392 kb on - strand, within sdhB at 757.392 kb on - strand, within sdhB at 757.488 kb on + strand, within sdhB at 757.517 kb on - strand, within sdhB at 757.517 kb on - strand, within sdhB at 757.536 kb on + strand at 757.536 kb on + strand, within sdhB at 757.536 kb on - strand, within sdhB at 757.544 kb on + strand, within sdhB at 757.544 kb on - strand, within sdhB at 757.566 kb on - strand at 757.566 kb on - strand at 757.673 kb on + strand at 757.681 kb on - strand at 757.703 kb on + strand at 757.713 kb on - strand at 757.713 kb on - strand at 757.791 kb on + strand at 757.929 kb on - strand at 758.303 kb on + strand, within sucA at 758.313 kb on + strand, within sucA at 759.115 kb on + strand, within sucA at 759.151 kb on + strand, within sucA at 759.205 kb on + strand, within sucA at 759.205 kb on + strand, within sucA at 759.270 kb on + strand, within sucA at 759.270 kb on + strand, within sucA at 759.633 kb on + strand, within sucA at 759.888 kb on + strand, within sucA at 759.888 kb on + strand, within sucA at 759.918 kb on + strand, within sucA at 759.959 kb on + strand, within sucA at 759.961 kb on + strand, within sucA at 759.961 kb on + strand, within sucA at 760.247 kb on + strand, within sucA at 760.469 kb on + strand at 760.665 kb on + strand at 760.667 kb on + strand at 760.880 kb on + strand at 760.880 kb on + strand, within sucB at 761.505 kb on + strand, within sucB at 761.728 kb on + strand, within sucB
Per-strain Table
Position Strand Gene LocusTag Fraction 20 hr rep1_No_BD100_grewON remove 757,053 + sdhB b0724 0.20 -0.9 757,138 - sdhB b0724 0.32 -0.1 757,143 - sdhB b0724 0.32 -0.5 757,184 - sdhB b0724 0.38 +0.4 757,198 + sdhB b0724 0.40 -0.6 757,198 + sdhB b0724 0.40 -1.3 757,229 - sdhB b0724 0.44 -1.0 757,233 + sdhB b0724 0.45 -1.4 757,241 - sdhB b0724 0.46 -0.4 757,381 - sdhB b0724 0.65 -1.2 757,392 - sdhB b0724 0.67 -1.3 757,392 - sdhB b0724 0.67 -0.2 757,488 + sdhB b0724 0.80 -0.8 757,517 - sdhB b0724 0.84 +0.0 757,517 - sdhB b0724 0.84 -0.6 757,536 + -0.5 757,536 + sdhB b0724 0.87 -1.2 757,536 - sdhB b0724 0.87 +0.0 757,544 + sdhB b0724 0.88 -1.3 757,544 - sdhB b0724 0.88 +0.0 757,566 - +1.3 757,566 - -1.5 757,673 + +0.0 757,681 - +0.2 757,703 + +0.3 757,713 - -1.1 757,713 - +0.6 757,791 + +0.3 757,929 - -2.2 758,303 + sucA b0726 0.13 -2.8 758,313 + sucA b0726 0.14 +0.3 759,115 + sucA b0726 0.42 -0.7 759,151 + sucA b0726 0.44 -1.8 759,205 + sucA b0726 0.46 -2.9 759,205 + sucA b0726 0.46 -0.3 759,270 + sucA b0726 0.48 -1.1 759,270 + sucA b0726 0.48 -2.5 759,633 + sucA b0726 0.61 -2.3 759,888 + sucA b0726 0.70 -0.9 759,888 + sucA b0726 0.70 -1.6 759,918 + sucA b0726 0.71 -4.6 759,959 + sucA b0726 0.72 -3.5 759,961 + sucA b0726 0.73 +1.1 759,961 + sucA b0726 0.73 -1.7 760,247 + sucA b0726 0.83 -0.2 760,469 + -0.7 760,665 + -0.7 760,667 + +0.4 760,880 + -0.7 760,880 + sucB b0727 0.11 -0.6 761,505 + sucB b0727 0.62 -0.7 761,728 + sucB b0727 0.81 -0.5
Or see this region's nucleotide sequence