Experiment: 20 hr rep1_No_BD100_grewON
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ybeF and lipB are separated by 258 nucleotides lipB and ybeD are separated by 100 nucleotides ybeD and dacA are separated by 109 nucleotides
b0629: ybeF - putative transcriptional regulator LYSR-type (VIMSS), at 659,648 to 660,601
ybeF
b0630: lipB - protein of lipoate biosynthesis (VIMSS), at 660,860 to 661,501
lipB
b0631: ybeD - hypothetical protein (NCBI), at 661,602 to 661,865
ybeD
b0632: dacA - D-alanyl-D-alanine carboxypeptidase (penicillin-binding protein 5) (NCBI), at 661,975 to 663,186
dacA
Position (kb) 660
661
662 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 660.067 kb on + strand, within ybeF at 660.067 kb on - strand, within ybeF at 660.116 kb on - strand, within ybeF at 660.209 kb on + strand, within ybeF at 660.233 kb on + strand, within ybeF at 660.260 kb on + strand, within ybeF at 660.260 kb on + strand, within ybeF at 660.273 kb on + strand, within ybeF at 660.281 kb on - strand, within ybeF at 660.281 kb on - strand, within ybeF at 660.298 kb on + strand, within ybeF at 660.298 kb on + strand, within ybeF at 660.308 kb on - strand, within ybeF at 660.310 kb on + strand, within ybeF at 660.317 kb on + strand, within ybeF at 660.322 kb on + strand, within ybeF at 660.326 kb on - strand, within ybeF at 660.327 kb on - strand, within ybeF at 660.327 kb on - strand, within ybeF at 660.330 kb on - strand, within ybeF at 660.343 kb on + strand, within ybeF at 660.343 kb on + strand, within ybeF at 660.349 kb on + strand, within ybeF at 660.350 kb on + strand, within ybeF at 660.351 kb on + strand, within ybeF at 660.351 kb on - strand, within ybeF at 660.351 kb on - strand, within ybeF at 660.354 kb on + strand, within ybeF at 660.354 kb on + strand, within ybeF at 660.356 kb on + strand, within ybeF at 660.356 kb on + strand, within ybeF at 660.359 kb on - strand, within ybeF at 660.359 kb on - strand, within ybeF at 660.364 kb on - strand, within ybeF at 660.401 kb on - strand, within ybeF at 660.401 kb on - strand, within ybeF at 660.403 kb on + strand, within ybeF at 660.403 kb on + strand, within ybeF at 660.403 kb on + strand, within ybeF at 660.403 kb on + strand, within ybeF at 660.411 kb on - strand, within ybeF at 660.417 kb on - strand, within ybeF at 660.420 kb on + strand, within ybeF at 660.432 kb on - strand, within ybeF at 660.439 kb on + strand, within ybeF at 660.476 kb on + strand, within ybeF at 660.476 kb on + strand, within ybeF at 660.476 kb on + strand, within ybeF at 660.476 kb on + strand, within ybeF at 660.479 kb on + strand, within ybeF at 660.479 kb on + strand, within ybeF at 660.505 kb on + strand, within ybeF at 660.505 kb on + strand, within ybeF at 660.506 kb on + strand at 660.527 kb on + strand at 660.543 kb on + strand at 660.543 kb on + strand at 660.552 kb on + strand at 660.596 kb on + strand at 660.730 kb on - strand at 660.850 kb on - strand at 660.850 kb on - strand at 661.084 kb on - strand, within lipB at 661.146 kb on + strand, within lipB at 661.264 kb on + strand, within lipB at 661.272 kb on - strand, within lipB at 661.509 kb on + strand at 661.519 kb on - strand at 661.957 kb on - strand at 661.957 kb on - strand at 662.131 kb on + strand, within dacA at 662.153 kb on - strand, within dacA at 662.155 kb on + strand, within dacA at 662.160 kb on + strand, within dacA at 662.160 kb on + strand, within dacA at 662.255 kb on + strand, within dacA at 662.380 kb on - strand, within dacA at 662.380 kb on - strand, within dacA at 662.462 kb on - strand, within dacA at 662.462 kb on - strand, within dacA
Per-strain Table
Position Strand Gene LocusTag Fraction 20 hr rep1_No_BD100_grewON remove 660,067 + ybeF b0629 0.44 -0.4 660,067 - ybeF b0629 0.44 +0.5 660,116 - ybeF b0629 0.49 +0.5 660,209 + ybeF b0629 0.59 -0.4 660,233 + ybeF b0629 0.61 +0.3 660,260 + ybeF b0629 0.64 +1.0 660,260 + ybeF b0629 0.64 +0.3 660,273 + ybeF b0629 0.66 +0.7 660,281 - ybeF b0629 0.66 +0.7 660,281 - ybeF b0629 0.66 -3.6 660,298 + ybeF b0629 0.68 -0.5 660,298 + ybeF b0629 0.68 -1.3 660,308 - ybeF b0629 0.69 +1.2 660,310 + ybeF b0629 0.69 +0.1 660,317 + ybeF b0629 0.70 -0.1 660,322 + ybeF b0629 0.71 +0.7 660,326 - ybeF b0629 0.71 +0.1 660,327 - ybeF b0629 0.71 +1.0 660,327 - ybeF b0629 0.71 +0.5 660,330 - ybeF b0629 0.71 +0.7 660,343 + ybeF b0629 0.73 -0.4 660,343 + ybeF b0629 0.73 -1.5 660,349 + ybeF b0629 0.73 -0.1 660,350 + ybeF b0629 0.74 +0.3 660,351 + ybeF b0629 0.74 +0.3 660,351 - ybeF b0629 0.74 -0.3 660,351 - ybeF b0629 0.74 -0.5 660,354 + ybeF b0629 0.74 +0.8 660,354 + ybeF b0629 0.74 -0.6 660,356 + ybeF b0629 0.74 +0.1 660,356 + ybeF b0629 0.74 -1.7 660,359 - ybeF b0629 0.75 -1.3 660,359 - ybeF b0629 0.75 -0.0 660,364 - ybeF b0629 0.75 -0.8 660,401 - ybeF b0629 0.79 +1.2 660,401 - ybeF b0629 0.79 +3.2 660,403 + ybeF b0629 0.79 -1.3 660,403 + ybeF b0629 0.79 -0.6 660,403 + ybeF b0629 0.79 +0.2 660,403 + ybeF b0629 0.79 +0.4 660,411 - ybeF b0629 0.80 +1.4 660,417 - ybeF b0629 0.81 +1.2 660,420 + ybeF b0629 0.81 +0.5 660,432 - ybeF b0629 0.82 +1.2 660,439 + ybeF b0629 0.83 +0.5 660,476 + ybeF b0629 0.87 +2.0 660,476 + ybeF b0629 0.87 +0.0 660,476 + ybeF b0629 0.87 -0.5 660,476 + ybeF b0629 0.87 +0.1 660,479 + ybeF b0629 0.87 +0.0 660,479 + ybeF b0629 0.87 +0.6 660,505 + ybeF b0629 0.90 +2.2 660,505 + ybeF b0629 0.90 -1.2 660,506 + +0.4 660,527 + -0.6 660,543 + -1.3 660,543 + -1.6 660,552 + -1.0 660,596 + -0.7 660,730 - +0.2 660,850 - -0.4 660,850 - +1.2 661,084 - lipB b0630 0.35 -1.0 661,146 + lipB b0630 0.45 -2.2 661,264 + lipB b0630 0.63 -1.5 661,272 - lipB b0630 0.64 -0.8 661,509 + +0.1 661,519 - +0.0 661,957 - -0.0 661,957 - +0.9 662,131 + dacA b0632 0.13 -0.5 662,153 - dacA b0632 0.15 -1.8 662,155 + dacA b0632 0.15 -2.5 662,160 + dacA b0632 0.15 +0.6 662,160 + dacA b0632 0.15 +0.4 662,255 + dacA b0632 0.23 -0.4 662,380 - dacA b0632 0.33 +0.8 662,380 - dacA b0632 0.33 +0.9 662,462 - dacA b0632 0.40 -0.5 662,462 - dacA b0632 0.40 +0.0
Or see this region's nucleotide sequence