Experiment: 20 hr rep1_No_BD100_grewON
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yadN and folK are separated by 369 nucleotides folK and pcnB overlap by 4 nucleotides
b0141: yadN - predicted fimbrial-like adhesin protein (NCBI), at 156,299 to 156,883
yadN
b0142: folK - 2-amino-4-hydroxy-6-hydroxymethyldihyropteridine pyrophosphokinase (NCBI), at 157,253 to 157,732
folK
b0143: pcnB - poly(A) polymerase I (VIMSS), at 157,729 to 159,126
pcnB
Position (kb)
157
158 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 156.298 kb on - strand at 156.298 kb on - strand at 156.298 kb on - strand at 156.298 kb on - strand at 156.298 kb on - strand at 156.298 kb on - strand at 156.298 kb on - strand at 156.312 kb on - strand at 156.322 kb on - strand at 156.322 kb on - strand at 156.322 kb on - strand at 156.324 kb on - strand at 156.324 kb on - strand at 156.324 kb on - strand at 156.324 kb on - strand at 156.324 kb on - strand at 156.324 kb on - strand at 156.324 kb on - strand at 156.324 kb on - strand at 156.324 kb on - strand at 156.324 kb on - strand at 156.332 kb on - strand at 156.332 kb on - strand at 156.356 kb on - strand at 156.356 kb on - strand at 156.356 kb on - strand at 156.356 kb on - strand at 156.359 kb on - strand, within yadN at 156.361 kb on - strand, within yadN at 156.367 kb on - strand, within yadN at 156.376 kb on - strand, within yadN at 156.376 kb on - strand, within yadN at 156.427 kb on - strand, within yadN at 156.428 kb on - strand, within yadN at 156.428 kb on - strand, within yadN at 156.429 kb on + strand, within yadN at 156.429 kb on + strand, within yadN at 156.431 kb on + strand, within yadN at 156.431 kb on + strand, within yadN at 156.432 kb on + strand, within yadN at 156.432 kb on + strand, within yadN at 156.437 kb on - strand, within yadN at 156.437 kb on - strand, within yadN at 156.437 kb on - strand, within yadN at 156.437 kb on - strand, within yadN at 156.437 kb on - strand, within yadN at 156.437 kb on - strand, within yadN at 156.437 kb on - strand, within yadN at 156.439 kb on - strand, within yadN at 156.439 kb on - strand, within yadN at 156.439 kb on - strand, within yadN at 156.439 kb on - strand, within yadN at 156.439 kb on - strand, within yadN at 156.440 kb on - strand, within yadN at 156.486 kb on + strand, within yadN at 156.489 kb on - strand, within yadN at 156.489 kb on - strand, within yadN at 156.521 kb on - strand, within yadN at 156.533 kb on - strand, within yadN at 156.545 kb on - strand, within yadN at 156.547 kb on - strand, within yadN at 156.560 kb on - strand, within yadN at 156.600 kb on + strand, within yadN at 156.602 kb on + strand, within yadN at 156.605 kb on - strand, within yadN at 156.642 kb on + strand, within yadN at 156.661 kb on + strand, within yadN at 156.682 kb on - strand, within yadN at 156.682 kb on - strand, within yadN at 156.685 kb on - strand, within yadN at 156.685 kb on - strand, within yadN at 156.685 kb on - strand, within yadN at 156.687 kb on - strand, within yadN at 156.705 kb on + strand, within yadN at 156.711 kb on + strand, within yadN at 156.721 kb on + strand, within yadN at 156.721 kb on + strand, within yadN at 156.721 kb on + strand, within yadN at 156.722 kb on + strand, within yadN at 156.734 kb on + strand, within yadN at 156.735 kb on + strand, within yadN at 156.735 kb on + strand, within yadN at 156.744 kb on + strand, within yadN at 156.755 kb on + strand, within yadN at 156.757 kb on + strand, within yadN at 156.759 kb on + strand, within yadN at 156.786 kb on + strand, within yadN at 156.789 kb on + strand, within yadN at 156.793 kb on + strand, within yadN at 156.804 kb on + strand, within yadN at 156.823 kb on + strand, within yadN at 156.823 kb on + strand, within yadN at 156.825 kb on + strand at 156.827 kb on + strand at 156.827 kb on + strand at 156.834 kb on + strand at 156.849 kb on + strand at 156.849 kb on + strand at 156.849 kb on + strand at 156.850 kb on + strand at 156.854 kb on + strand at 156.854 kb on + strand at 157.218 kb on - strand at 157.279 kb on - strand at 157.291 kb on - strand at 157.301 kb on - strand, within folK at 157.745 kb on + strand at 157.870 kb on - strand, within pcnB at 157.906 kb on - strand, within pcnB at 157.906 kb on - strand, within pcnB at 157.923 kb on - strand, within pcnB at 158.143 kb on + strand, within pcnB at 158.156 kb on + strand, within pcnB at 158.227 kb on - strand, within pcnB at 158.249 kb on - strand, within pcnB at 158.249 kb on - strand, within pcnB at 158.312 kb on - strand, within pcnB at 158.342 kb on - strand, within pcnB at 158.378 kb on - strand, within pcnB at 158.400 kb on + strand, within pcnB at 158.408 kb on - strand, within pcnB at 158.408 kb on - strand, within pcnB at 158.426 kb on - strand, within pcnB at 158.442 kb on - strand, within pcnB at 158.442 kb on - strand, within pcnB at 158.447 kb on - strand, within pcnB at 158.516 kb on + strand, within pcnB at 158.522 kb on - strand, within pcnB
Per-strain Table
Position Strand Gene LocusTag Fraction 20 hr rep1_No_BD100_grewON remove 156,298 - -1.5 156,298 - +0.3 156,298 - +0.4 156,298 - +0.8 156,298 - -0.3 156,298 - -0.8 156,298 - +0.2 156,312 - -1.5 156,322 - -0.3 156,322 - -1.2 156,322 - +0.1 156,324 - +1.0 156,324 - +0.9 156,324 - +0.2 156,324 - +0.2 156,324 - -0.5 156,324 - -1.4 156,324 - +1.0 156,324 - -0.5 156,324 - -0.7 156,324 - -1.4 156,332 - -0.1 156,332 - -0.4 156,356 - -0.3 156,356 - +0.2 156,356 - -1.8 156,356 - -2.8 156,359 - yadN b0141 0.10 +0.6 156,361 - yadN b0141 0.11 -1.2 156,367 - yadN b0141 0.12 -0.7 156,376 - yadN b0141 0.13 -0.6 156,376 - yadN b0141 0.13 -0.1 156,427 - yadN b0141 0.22 -1.1 156,428 - yadN b0141 0.22 +1.6 156,428 - yadN b0141 0.22 -1.2 156,429 + yadN b0141 0.22 +0.4 156,429 + yadN b0141 0.22 +1.4 156,431 + yadN b0141 0.23 +1.4 156,431 + yadN b0141 0.23 +1.4 156,432 + yadN b0141 0.23 +2.1 156,432 + yadN b0141 0.23 -0.6 156,437 - yadN b0141 0.24 +0.4 156,437 - yadN b0141 0.24 +1.4 156,437 - yadN b0141 0.24 +1.0 156,437 - yadN b0141 0.24 +0.1 156,437 - yadN b0141 0.24 -0.4 156,437 - yadN b0141 0.24 -0.4 156,437 - yadN b0141 0.24 +1.4 156,439 - yadN b0141 0.24 -1.5 156,439 - yadN b0141 0.24 +0.0 156,439 - yadN b0141 0.24 +0.2 156,439 - yadN b0141 0.24 +2.5 156,439 - yadN b0141 0.24 -0.5 156,440 - yadN b0141 0.24 +1.4 156,486 + yadN b0141 0.32 +1.4 156,489 - yadN b0141 0.32 -2.4 156,489 - yadN b0141 0.32 +2.0 156,521 - yadN b0141 0.38 +1.0 156,533 - yadN b0141 0.40 +0.4 156,545 - yadN b0141 0.42 +0.9 156,547 - yadN b0141 0.42 -2.5 156,560 - yadN b0141 0.45 -2.4 156,600 + yadN b0141 0.51 +1.4 156,602 + yadN b0141 0.52 +1.4 156,605 - yadN b0141 0.52 +0.8 156,642 + yadN b0141 0.59 +1.4 156,661 + yadN b0141 0.62 -0.2 156,682 - yadN b0141 0.65 -1.2 156,682 - yadN b0141 0.65 +0.3 156,685 - yadN b0141 0.66 +1.4 156,685 - yadN b0141 0.66 -0.2 156,685 - yadN b0141 0.66 +0.3 156,687 - yadN b0141 0.66 -0.2 156,705 + yadN b0141 0.69 -1.7 156,711 + yadN b0141 0.70 +1.4 156,721 + yadN b0141 0.72 -1.8 156,721 + yadN b0141 0.72 -0.9 156,721 + yadN b0141 0.72 -0.6 156,722 + yadN b0141 0.72 +3.5 156,734 + yadN b0141 0.74 +2.0 156,735 + yadN b0141 0.75 +0.9 156,735 + yadN b0141 0.75 +0.3 156,744 + yadN b0141 0.76 +0.4 156,755 + yadN b0141 0.78 -0.2 156,757 + yadN b0141 0.78 +0.4 156,759 + yadN b0141 0.79 +0.7 156,786 + yadN b0141 0.83 +0.4 156,789 + yadN b0141 0.84 -0.5 156,793 + yadN b0141 0.84 -0.6 156,804 + yadN b0141 0.86 +0.3 156,823 + yadN b0141 0.90 +1.4 156,823 + yadN b0141 0.90 -1.2 156,825 + +0.8 156,827 + -0.0 156,827 + -0.4 156,834 + +2.1 156,849 + +1.9 156,849 + +1.2 156,849 + +0.3 156,850 + -0.4 156,854 + -0.0 156,854 + -0.6 157,218 - +0.4 157,279 - -0.8 157,291 - -2.9 157,301 - folK b0142 0.10 -2.1 157,745 + -1.3 157,870 - pcnB b0143 0.10 -0.1 157,906 - pcnB b0143 0.13 +0.8 157,906 - pcnB b0143 0.13 +1.6 157,923 - pcnB b0143 0.14 +0.5 158,143 + pcnB b0143 0.30 +0.1 158,156 + pcnB b0143 0.31 -0.6 158,227 - pcnB b0143 0.36 -0.2 158,249 - pcnB b0143 0.37 -1.8 158,249 - pcnB b0143 0.37 -0.7 158,312 - pcnB b0143 0.42 -2.0 158,342 - pcnB b0143 0.44 -0.3 158,378 - pcnB b0143 0.46 -0.9 158,400 + pcnB b0143 0.48 -2.2 158,408 - pcnB b0143 0.49 -3.1 158,408 - pcnB b0143 0.49 +2.1 158,426 - pcnB b0143 0.50 +0.4 158,442 - pcnB b0143 0.51 -2.4 158,442 - pcnB b0143 0.51 -0.1 158,447 - pcnB b0143 0.51 +0.2 158,516 + pcnB b0143 0.56 -0.7 158,522 - pcnB b0143 0.57 -0.3
Or see this region's nucleotide sequence