Strain Fitness in Escherichia coli BW25113 around b2918

Experiment: 6 hr rep2_HD100_10fold_dilution_grewON

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyliK and argK overlap by 8 nucleotidesargK and ygfG are separated by 10 nucleotidesygfG and ygfH are separated by 23 nucleotides b2917: yliK - methylmalonyl-CoA mutase (NCBI), at 3,058,872 to 3,061,016 yliK b2918: argK - arginine/ornithine transport system ATPase (NCBI), at 3,061,009 to 3,062,004 argK b2919: ygfG - putative enzyme (VIMSS), at 3,062,015 to 3,062,800 ygfG b2920: ygfH - propionyl-CoA:succinate-CoA transferase (NCBI), at 3,062,824 to 3,064,302 ygfH Position (kb) 3061 3062 3063Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1 2at 3060.026 kb on + strand, within yliKat 3060.064 kb on - strand, within yliKat 3060.068 kb on + strand, within yliKat 3060.151 kb on + strand, within yliKat 3060.215 kb on + strand, within yliKat 3060.350 kb on - strand, within yliKat 3060.369 kb on + strand, within yliKat 3060.445 kb on + strand, within yliKat 3060.458 kb on - strand, within yliKat 3060.479 kb on - strand, within yliKat 3060.515 kb on - strand, within yliKat 3060.592 kb on - strand, within yliKat 3060.592 kb on - strand, within yliKat 3060.662 kb on - strand, within yliKat 3060.741 kb on + strand, within yliKat 3060.741 kb on + strand, within yliKat 3060.826 kb on + strandat 3060.826 kb on + strandat 3060.926 kb on + strandat 3060.958 kb on + strandat 3060.958 kb on + strandat 3060.963 kb on + strandat 3061.019 kb on + strandat 3061.019 kb on + strandat 3061.082 kb on - strandat 3061.088 kb on - strandat 3061.127 kb on + strand, within argKat 3061.127 kb on + strand, within argKat 3061.292 kb on - strand, within argKat 3061.292 kb on - strand, within argKat 3061.372 kb on + strand, within argKat 3061.387 kb on + strand, within argKat 3061.397 kb on + strand, within argKat 3061.561 kb on - strand, within argKat 3061.654 kb on + strand, within argKat 3061.654 kb on + strand, within argKat 3062.118 kb on + strand, within ygfGat 3062.216 kb on + strand, within ygfGat 3062.251 kb on + strand, within ygfGat 3062.256 kb on + strand, within ygfGat 3062.256 kb on + strand, within ygfGat 3062.328 kb on + strand, within ygfGat 3062.328 kb on + strand, within ygfGat 3062.364 kb on + strand, within ygfGat 3062.390 kb on + strand, within ygfGat 3062.411 kb on + strand, within ygfGat 3062.438 kb on - strand, within ygfGat 3062.438 kb on - strand, within ygfGat 3062.608 kb on - strand, within ygfGat 3062.608 kb on - strand, within ygfGat 3062.651 kb on + strand, within ygfGat 3062.723 kb on + strandat 3062.731 kb on - strandat 3062.731 kb on - strandat 3062.785 kb on - strandat 3062.929 kb on + strandat 3062.937 kb on - strandat 3062.937 kb on - strandat 3062.937 kb on - strandat 3062.963 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 6 hr rep2_HD100_10fold_dilution_grewON
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3,060,026 + yliK b2917 0.54 -4.1
3,060,064 - yliK b2917 0.56 +0.4
3,060,068 + yliK b2917 0.56 -5.2
3,060,151 + yliK b2917 0.60 -0.2
3,060,215 + yliK b2917 0.63 +1.2
3,060,350 - yliK b2917 0.69 +1.0
3,060,369 + yliK b2917 0.70 -3.4
3,060,445 + yliK b2917 0.73 +0.3
3,060,458 - yliK b2917 0.74 +0.7
3,060,479 - yliK b2917 0.75 -0.3
3,060,515 - yliK b2917 0.77 +0.4
3,060,592 - yliK b2917 0.80 +1.4
3,060,592 - yliK b2917 0.80 -5.0
3,060,662 - yliK b2917 0.83 -5.1
3,060,741 + yliK b2917 0.87 -0.1
3,060,741 + yliK b2917 0.87 -0.5
3,060,826 + +1.6
3,060,826 + +0.3
3,060,926 + +0.7
3,060,958 + +1.8
3,060,958 + +1.4
3,060,963 + -0.1
3,061,019 + -3.9
3,061,019 + +1.1
3,061,082 - +1.2
3,061,088 - +0.8
3,061,127 + argK b2918 0.12 +0.9
3,061,127 + argK b2918 0.12 +0.7
3,061,292 - argK b2918 0.28 -0.2
3,061,292 - argK b2918 0.28 +2.2
3,061,372 + argK b2918 0.36 +1.5
3,061,387 + argK b2918 0.38 +1.8
3,061,397 + argK b2918 0.39 -0.2
3,061,561 - argK b2918 0.55 +2.1
3,061,654 + argK b2918 0.65 +0.6
3,061,654 + argK b2918 0.65 -4.6
3,062,118 + ygfG b2919 0.13 -4.1
3,062,216 + ygfG b2919 0.26 -4.4
3,062,251 + ygfG b2919 0.30 -4.2
3,062,256 + ygfG b2919 0.31 -4.9
3,062,256 + ygfG b2919 0.31 -4.6
3,062,328 + ygfG b2919 0.40 -4.1
3,062,328 + ygfG b2919 0.40 -3.5
3,062,364 + ygfG b2919 0.44 +0.4
3,062,390 + ygfG b2919 0.48 +0.8
3,062,411 + ygfG b2919 0.50 +1.2
3,062,438 - ygfG b2919 0.54 -2.1
3,062,438 - ygfG b2919 0.54 +0.6
3,062,608 - ygfG b2919 0.75 -2.1
3,062,608 - ygfG b2919 0.75 -4.1
3,062,651 + ygfG b2919 0.81 +0.0
3,062,723 + -0.7
3,062,731 - -0.5
3,062,731 - +0.9
3,062,785 - -0.3
3,062,929 + +0.3
3,062,937 - +0.7
3,062,937 - -1.1
3,062,937 - -0.9
3,062,963 - -0.2

Or see this region's nucleotide sequence