Strain Fitness in Escherichia coli BW25113 around b2374

Experiment: 6 hr rep2_HD100_10fold_dilution_grewON

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntoxc and frc are separated by 53 nucleotidesfrc and yfdX are separated by 512 nucleotides b2373: oxc - hypothetical protein (NCBI), at 2,488,278 to 2,489,972 oxc b2374: frc - formyl-coenzyme A transferase (NCBI), at 2,490,026 to 2,491,276 frc b2375: yfdX - hypothetical protein (NCBI), at 2,491,789 to 2,492,424 yfdX Position (kb) 2490 2491 2492Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1 2 3at 2489.068 kb on - strand, within oxcat 2489.068 kb on - strand, within oxcat 2489.073 kb on - strand, within oxcat 2489.132 kb on + strand, within oxcat 2489.153 kb on - strand, within oxcat 2489.195 kb on + strand, within oxcat 2489.216 kb on - strand, within oxcat 2489.216 kb on - strand, within oxcat 2489.218 kb on - strand, within oxcat 2489.237 kb on + strand, within oxcat 2489.246 kb on - strand, within oxcat 2489.248 kb on + strand, within oxcat 2489.248 kb on + strand, within oxcat 2489.293 kb on - strand, within oxcat 2489.293 kb on - strand, within oxcat 2489.326 kb on + strand, within oxcat 2489.370 kb on - strand, within oxcat 2489.370 kb on - strand, within oxcat 2489.434 kb on - strand, within oxcat 2489.434 kb on - strand, within oxcat 2489.468 kb on - strand, within oxcat 2489.491 kb on + strand, within oxcat 2489.491 kb on + strand, within oxcat 2489.504 kb on + strand, within oxcat 2489.509 kb on + strand, within oxcat 2489.509 kb on + strand, within oxcat 2489.513 kb on - strand, within oxcat 2489.529 kb on + strand, within oxcat 2489.537 kb on - strand, within oxcat 2489.644 kb on + strand, within oxcat 2489.662 kb on - strand, within oxcat 2489.786 kb on + strand, within oxcat 2489.793 kb on + strand, within oxcat 2489.793 kb on + strand, within oxcat 2489.798 kb on + strand, within oxcat 2489.805 kb on + strandat 2489.810 kb on + strandat 2489.822 kb on + strandat 2489.850 kb on + strandat 2489.855 kb on + strandat 2489.855 kb on + strandat 2489.859 kb on + strandat 2489.859 kb on + strandat 2489.880 kb on + strandat 2489.880 kb on + strandat 2489.880 kb on + strandat 2489.934 kb on + strandat 2489.936 kb on + strandat 2489.939 kb on + strandat 2489.939 kb on + strandat 2489.948 kb on + strandat 2490.023 kb on + strandat 2490.029 kb on - strandat 2490.082 kb on - strandat 2490.178 kb on - strand, within frcat 2490.200 kb on + strand, within frcat 2490.208 kb on - strand, within frcat 2490.211 kb on - strand, within frcat 2490.276 kb on - strand, within frcat 2490.322 kb on - strand, within frcat 2490.322 kb on - strand, within frcat 2490.384 kb on + strand, within frcat 2490.390 kb on - strand, within frcat 2490.390 kb on - strand, within frcat 2490.390 kb on - strand, within frcat 2490.390 kb on - strand, within frcat 2490.392 kb on - strand, within frcat 2490.392 kb on - strand, within frcat 2490.392 kb on - strand, within frcat 2490.392 kb on - strand, within frcat 2490.397 kb on - strand, within frcat 2490.397 kb on - strand, within frcat 2490.397 kb on - strand, within frcat 2490.398 kb on - strandat 2490.398 kb on - strand, within frcat 2490.398 kb on - strand, within frcat 2490.400 kb on - strand, within frcat 2490.400 kb on - strand, within frcat 2490.400 kb on - strand, within frcat 2490.402 kb on - strand, within frcat 2490.402 kb on - strand, within frcat 2490.458 kb on - strand, within frcat 2490.527 kb on - strand, within frcat 2490.527 kb on - strand, within frcat 2490.529 kb on + strand, within frcat 2490.541 kb on + strand, within frcat 2490.541 kb on + strand, within frcat 2490.543 kb on + strand, within frcat 2490.546 kb on - strand, within frcat 2490.552 kb on - strand, within frcat 2490.552 kb on - strand, within frcat 2490.554 kb on - strand, within frcat 2490.579 kb on - strand, within frcat 2490.590 kb on + strand, within frcat 2490.600 kb on - strand, within frcat 2490.606 kb on + strand, within frcat 2490.615 kb on + strand, within frcat 2490.681 kb on + strand, within frcat 2490.681 kb on + strand, within frcat 2490.689 kb on - strand, within frcat 2490.692 kb on - strand, within frcat 2490.873 kb on - strand, within frcat 2490.875 kb on - strand, within frcat 2490.875 kb on - strand, within frcat 2490.953 kb on + strand, within frcat 2490.961 kb on - strand, within frcat 2490.962 kb on + strand, within frcat 2490.967 kb on + strand, within frcat 2490.976 kb on + strand, within frcat 2490.976 kb on + strand, within frcat 2491.026 kb on + strand, within frcat 2491.026 kb on + strand, within frcat 2491.034 kb on + strand, within frcat 2491.034 kb on + strand, within frcat 2491.082 kb on + strand, within frcat 2491.085 kb on + strand, within frcat 2491.093 kb on - strand, within frcat 2491.100 kb on + strand, within frcat 2491.100 kb on + strand, within frcat 2491.100 kb on + strand, within frcat 2491.124 kb on + strand, within frcat 2491.135 kb on + strand, within frcat 2491.135 kb on + strand, within frcat 2491.144 kb on + strand, within frcat 2491.147 kb on + strand, within frcat 2491.148 kb on + strand, within frcat 2491.158 kb on - strandat 2491.163 kb on - strandat 2491.180 kb on + strandat 2491.214 kb on + strandat 2491.214 kb on + strandat 2491.223 kb on + strandat 2491.223 kb on + strandat 2491.225 kb on + strandat 2491.225 kb on + strandat 2491.751 kb on - strandat 2491.751 kb on - strandat 2491.778 kb on - strandat 2491.778 kb on - strandat 2491.801 kb on - strandat 2491.821 kb on - strandat 2491.821 kb on - strandat 2491.905 kb on + strand, within yfdXat 2491.911 kb on - strand, within yfdXat 2491.925 kb on - strand, within yfdXat 2491.950 kb on + strand, within yfdXat 2491.956 kb on - strand, within yfdXat 2492.060 kb on - strand, within yfdXat 2492.060 kb on - strand, within yfdXat 2492.062 kb on - strand, within yfdXat 2492.102 kb on - strand, within yfdXat 2492.137 kb on - strand, within yfdXat 2492.153 kb on + strand, within yfdXat 2492.153 kb on + strand, within yfdXat 2492.159 kb on - strand, within yfdXat 2492.161 kb on - strand, within yfdXat 2492.183 kb on + strand, within yfdXat 2492.183 kb on + strand, within yfdXat 2492.221 kb on + strand, within yfdXat 2492.221 kb on + strand, within yfdXat 2492.227 kb on + strand, within yfdXat 2492.232 kb on + strand, within yfdXat 2492.269 kb on - strand, within yfdX

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Per-strain Table

Position Strand Gene LocusTag Fraction 6 hr rep2_HD100_10fold_dilution_grewON
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2,489,068 - oxc b2373 0.47 -1.1
2,489,068 - oxc b2373 0.47 -2.0
2,489,073 - oxc b2373 0.47 -2.4
2,489,132 + oxc b2373 0.50 +1.2
2,489,153 - oxc b2373 0.52 -4.2
2,489,195 + oxc b2373 0.54 -1.6
2,489,216 - oxc b2373 0.55 +2.1
2,489,216 - oxc b2373 0.55 -2.1
2,489,218 - oxc b2373 0.55 -3.4
2,489,237 + oxc b2373 0.57 +1.2
2,489,246 - oxc b2373 0.57 -1.1
2,489,248 + oxc b2373 0.57 +0.2
2,489,248 + oxc b2373 0.57 -2.0
2,489,293 - oxc b2373 0.60 +0.4
2,489,293 - oxc b2373 0.60 -3.8
2,489,326 + oxc b2373 0.62 -0.8
2,489,370 - oxc b2373 0.64 -3.6
2,489,370 - oxc b2373 0.64 -0.5
2,489,434 - oxc b2373 0.68 -4.2
2,489,434 - oxc b2373 0.68 +3.3
2,489,468 - oxc b2373 0.70 -4.1
2,489,491 + oxc b2373 0.72 -1.1
2,489,491 + oxc b2373 0.72 -0.4
2,489,504 + oxc b2373 0.72 -0.8
2,489,509 + oxc b2373 0.73 -1.6
2,489,509 + oxc b2373 0.73 -0.8
2,489,513 - oxc b2373 0.73 +2.2
2,489,529 + oxc b2373 0.74 -0.4
2,489,537 - oxc b2373 0.74 -4.0
2,489,644 + oxc b2373 0.81 -2.4
2,489,662 - oxc b2373 0.82 -2.1
2,489,786 + oxc b2373 0.89 -2.9
2,489,793 + oxc b2373 0.89 +1.1
2,489,793 + oxc b2373 0.89 -3.4
2,489,798 + oxc b2373 0.90 -2.4
2,489,805 + -1.4
2,489,810 + -2.7
2,489,822 + -0.0
2,489,850 + -2.1
2,489,855 + -3.4
2,489,855 + +1.3
2,489,859 + -3.1
2,489,859 + -2.8
2,489,880 + -3.1
2,489,880 + +1.3
2,489,880 + +1.3
2,489,934 + -3.6
2,489,936 + +1.0
2,489,939 + +0.6
2,489,939 + +1.1
2,489,948 + -3.0
2,490,023 + +1.2
2,490,029 - -2.3
2,490,082 - +0.2
2,490,178 - frc b2374 0.12 -3.5
2,490,200 + frc b2374 0.14 +0.2
2,490,208 - frc b2374 0.15 -1.4
2,490,211 - frc b2374 0.15 +1.1
2,490,276 - frc b2374 0.20 -1.6
2,490,322 - frc b2374 0.24 -1.8
2,490,322 - frc b2374 0.24 -2.9
2,490,384 + frc b2374 0.29 +1.2
2,490,390 - frc b2374 0.29 +1.1
2,490,390 - frc b2374 0.29 -3.5
2,490,390 - frc b2374 0.29 +2.0
2,490,390 - frc b2374 0.29 -4.9
2,490,392 - frc b2374 0.29 -4.5
2,490,392 - frc b2374 0.29 -4.5
2,490,392 - frc b2374 0.29 -2.5
2,490,392 - frc b2374 0.29 -1.7
2,490,397 - frc b2374 0.30 -1.3
2,490,397 - frc b2374 0.30 +0.1
2,490,397 - frc b2374 0.30 +1.4
2,490,398 - +1.2
2,490,398 - frc b2374 0.30 -3.6
2,490,398 - frc b2374 0.30 +0.9
2,490,400 - frc b2374 0.30 -1.4
2,490,400 - frc b2374 0.30 +0.2
2,490,400 - frc b2374 0.30 +0.2
2,490,402 - frc b2374 0.30 -1.4
2,490,402 - frc b2374 0.30 -0.2
2,490,458 - frc b2374 0.35 -4.1
2,490,527 - frc b2374 0.40 +0.0
2,490,527 - frc b2374 0.40 +1.1
2,490,529 + frc b2374 0.40 -0.8
2,490,541 + frc b2374 0.41 +0.2
2,490,541 + frc b2374 0.41 -3.1
2,490,543 + frc b2374 0.41 -0.4
2,490,546 - frc b2374 0.42 +0.8
2,490,552 - frc b2374 0.42 -1.3
2,490,552 - frc b2374 0.42 +0.4
2,490,554 - frc b2374 0.42 +0.1
2,490,579 - frc b2374 0.44 -4.7
2,490,590 + frc b2374 0.45 -4.0
2,490,600 - frc b2374 0.46 +0.4
2,490,606 + frc b2374 0.46 -2.0
2,490,615 + frc b2374 0.47 +2.2
2,490,681 + frc b2374 0.52 -2.6
2,490,681 + frc b2374 0.52 +0.5
2,490,689 - frc b2374 0.53 -4.8
2,490,692 - frc b2374 0.53 +0.3
2,490,873 - frc b2374 0.68 +1.1
2,490,875 - frc b2374 0.68 +0.4
2,490,875 - frc b2374 0.68 -4.6
2,490,953 + frc b2374 0.74 +0.9
2,490,961 - frc b2374 0.75 -3.8
2,490,962 + frc b2374 0.75 -1.3
2,490,967 + frc b2374 0.75 +0.3
2,490,976 + frc b2374 0.76 -2.7
2,490,976 + frc b2374 0.76 -3.3
2,491,026 + frc b2374 0.80 +1.9
2,491,026 + frc b2374 0.80 -0.4
2,491,034 + frc b2374 0.81 -1.1
2,491,034 + frc b2374 0.81 +0.9
2,491,082 + frc b2374 0.84 -2.0
2,491,085 + frc b2374 0.85 -0.8
2,491,093 - frc b2374 0.85 +0.2
2,491,100 + frc b2374 0.86 +0.2
2,491,100 + frc b2374 0.86 +1.2
2,491,100 + frc b2374 0.86 -1.8
2,491,124 + frc b2374 0.88 +0.2
2,491,135 + frc b2374 0.89 +0.2
2,491,135 + frc b2374 0.89 +1.2
2,491,144 + frc b2374 0.89 -0.0
2,491,147 + frc b2374 0.90 +0.2
2,491,148 + frc b2374 0.90 -1.1
2,491,158 - -0.4
2,491,163 - -2.3
2,491,180 + +1.2
2,491,214 + -0.8
2,491,214 + +1.2
2,491,223 + -3.4
2,491,223 + -0.4
2,491,225 + -0.4
2,491,225 + -0.4
2,491,751 - +0.2
2,491,751 - -1.8
2,491,778 - +2.4
2,491,778 - -2.4
2,491,801 - -1.1
2,491,821 - -3.1
2,491,821 - +1.4
2,491,905 + yfdX b2375 0.18 +1.2
2,491,911 - yfdX b2375 0.19 +1.3
2,491,925 - yfdX b2375 0.21 +1.2
2,491,950 + yfdX b2375 0.25 +1.2
2,491,956 - yfdX b2375 0.26 +2.2
2,492,060 - yfdX b2375 0.43 -0.4
2,492,060 - yfdX b2375 0.43 -1.4
2,492,062 - yfdX b2375 0.43 +1.2
2,492,102 - yfdX b2375 0.49 +0.2
2,492,137 - yfdX b2375 0.55 -3.9
2,492,153 + yfdX b2375 0.57 +1.2
2,492,153 + yfdX b2375 0.57 +1.2
2,492,159 - yfdX b2375 0.58 +2.2
2,492,161 - yfdX b2375 0.58 +2.4
2,492,183 + yfdX b2375 0.62 -0.4
2,492,183 + yfdX b2375 0.62 +1.2
2,492,221 + yfdX b2375 0.68 +0.2
2,492,221 + yfdX b2375 0.68 -0.8
2,492,227 + yfdX b2375 0.69 -0.8
2,492,232 + yfdX b2375 0.70 -4.3
2,492,269 - yfdX b2375 0.75 -2.1

Or see this region's nucleotide sequence