Experiment: 6 hr rep2_HD100_10fold_dilution_grewON
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt b2074 and mdtB overlap by 1 nucleotides mdtB and mdtC are separated by 0 nucleotides
b2074: b2074 - putative membrane protein (VIMSS), at 2,152,040 to 2,153,287
b2074
b2075: mdtB - multidrug efflux system, subunit B (NCBI), at 2,153,287 to 2,156,409
mdtB
b2076: mdtC - multidrug efflux system, subunit C (NCBI), at 2,156,410 to 2,159,487
mdtC
Position (kb)
2153
2154
2155
2156
2157 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1
2 at 2152.447 kb on + strand, within b2074 at 2152.677 kb on - strand, within b2074 at 2152.677 kb on - strand, within b2074 at 2152.748 kb on + strand, within b2074 at 2152.748 kb on + strand, within b2074 at 2153.105 kb on + strand, within b2074 at 2153.105 kb on + strand, within b2074 at 2153.113 kb on + strand, within b2074 at 2153.113 kb on - strand, within b2074 at 2153.113 kb on - strand, within b2074 at 2153.115 kb on + strand, within b2074 at 2153.343 kb on - strand at 2153.359 kb on - strand at 2153.359 kb on - strand at 2153.415 kb on - strand at 2153.471 kb on + strand at 2153.581 kb on + strand at 2153.581 kb on + strand at 2153.695 kb on + strand, within mdtB at 2153.695 kb on + strand, within mdtB at 2153.753 kb on + strand, within mdtB at 2153.753 kb on + strand, within mdtB at 2153.984 kb on + strand, within mdtB at 2154.025 kb on + strand, within mdtB at 2154.086 kb on + strand, within mdtB at 2154.103 kb on - strand, within mdtB at 2154.231 kb on + strand, within mdtB at 2154.240 kb on - strand, within mdtB at 2154.260 kb on + strand, within mdtB at 2154.260 kb on + strand, within mdtB at 2154.419 kb on + strand, within mdtB at 2154.583 kb on + strand, within mdtB at 2154.606 kb on - strand, within mdtB at 2154.606 kb on - strand, within mdtB at 2154.663 kb on + strand, within mdtB at 2154.709 kb on + strand, within mdtB at 2154.863 kb on + strand, within mdtB at 2154.926 kb on - strand, within mdtB at 2155.098 kb on - strand, within mdtB at 2155.135 kb on + strand, within mdtB at 2155.143 kb on - strand, within mdtB at 2155.143 kb on - strand, within mdtB at 2155.213 kb on + strand, within mdtB at 2155.213 kb on + strand, within mdtB at 2155.357 kb on - strand, within mdtB at 2155.480 kb on - strand, within mdtB at 2155.514 kb on - strand, within mdtB at 2155.543 kb on + strand, within mdtB at 2155.550 kb on - strand, within mdtB at 2155.566 kb on - strand, within mdtB at 2155.594 kb on - strand, within mdtB at 2155.630 kb on - strand, within mdtB at 2155.630 kb on - strand, within mdtB at 2155.761 kb on - strand, within mdtB at 2155.885 kb on + strand, within mdtB at 2155.919 kb on + strand, within mdtB at 2156.112 kb on - strand at 2156.167 kb on + strand at 2156.248 kb on - strand at 2156.265 kb on - strand at 2156.330 kb on - strand at 2156.357 kb on + strand at 2156.478 kb on + strand at 2156.511 kb on - strand at 2156.564 kb on + strand at 2156.564 kb on + strand at 2156.991 kb on + strand, within mdtC at 2157.092 kb on - strand, within mdtC at 2157.092 kb on - strand, within mdtC at 2157.158 kb on - strand, within mdtC at 2157.214 kb on - strand, within mdtC at 2157.272 kb on - strand, within mdtC at 2157.330 kb on + strand, within mdtC at 2157.378 kb on + strand, within mdtC
Per-strain Table
Position Strand Gene LocusTag Fraction 6 hr rep2_HD100_10fold_dilution_grewON remove 2,152,447 + b2074 b2074 0.33 +0.4 2,152,677 - b2074 b2074 0.51 -2.9 2,152,677 - b2074 b2074 0.51 -4.4 2,152,748 + b2074 b2074 0.57 +0.3 2,152,748 + b2074 b2074 0.57 -0.7 2,153,105 + b2074 b2074 0.85 +1.3 2,153,105 + b2074 b2074 0.85 +2.3 2,153,113 + b2074 b2074 0.86 -3.1 2,153,113 - b2074 b2074 0.86 -2.4 2,153,113 - b2074 b2074 0.86 -4.0 2,153,115 + b2074 b2074 0.86 +2.0 2,153,343 - +1.3 2,153,359 - -0.8 2,153,359 - +1.1 2,153,415 - +0.4 2,153,471 + +0.9 2,153,581 + -4.4 2,153,581 + -4.1 2,153,695 + mdtB b2075 0.13 -4.3 2,153,695 + mdtB b2075 0.13 +0.4 2,153,753 + mdtB b2075 0.15 +1.0 2,153,753 + mdtB b2075 0.15 -0.3 2,153,984 + mdtB b2075 0.22 +0.1 2,154,025 + mdtB b2075 0.24 +0.4 2,154,086 + mdtB b2075 0.26 -1.1 2,154,103 - mdtB b2075 0.26 +0.7 2,154,231 + mdtB b2075 0.30 +0.6 2,154,240 - mdtB b2075 0.31 +2.5 2,154,260 + mdtB b2075 0.31 -3.4 2,154,260 + mdtB b2075 0.31 -3.6 2,154,419 + mdtB b2075 0.36 +0.1 2,154,583 + mdtB b2075 0.41 -4.5 2,154,606 - mdtB b2075 0.42 -4.9 2,154,606 - mdtB b2075 0.42 -0.4 2,154,663 + mdtB b2075 0.44 +0.8 2,154,709 + mdtB b2075 0.46 -5.2 2,154,863 + mdtB b2075 0.50 -1.6 2,154,926 - mdtB b2075 0.52 -4.1 2,155,098 - mdtB b2075 0.58 -0.3 2,155,135 + mdtB b2075 0.59 -2.0 2,155,143 - mdtB b2075 0.59 -2.1 2,155,143 - mdtB b2075 0.59 +0.6 2,155,213 + mdtB b2075 0.62 -0.5 2,155,213 + mdtB b2075 0.62 +1.7 2,155,357 - mdtB b2075 0.66 +0.9 2,155,480 - mdtB b2075 0.70 +1.4 2,155,514 - mdtB b2075 0.71 +1.5 2,155,543 + mdtB b2075 0.72 -1.2 2,155,550 - mdtB b2075 0.72 -0.5 2,155,566 - mdtB b2075 0.73 -4.9 2,155,594 - mdtB b2075 0.74 -1.5 2,155,630 - mdtB b2075 0.75 -0.2 2,155,630 - mdtB b2075 0.75 +0.9 2,155,761 - mdtB b2075 0.79 -5.7 2,155,885 + mdtB b2075 0.83 -0.5 2,155,919 + mdtB b2075 0.84 -4.5 2,156,112 - +1.2 2,156,167 + -0.9 2,156,248 - +0.7 2,156,265 - +0.2 2,156,330 - +0.3 2,156,357 + +1.5 2,156,478 + +1.8 2,156,511 - +0.6 2,156,564 + +1.1 2,156,564 + +1.0 2,156,991 + mdtC b2076 0.19 +0.4 2,157,092 - mdtC b2076 0.22 +0.2 2,157,092 - mdtC b2076 0.22 -2.8 2,157,158 - mdtC b2076 0.24 +0.9 2,157,214 - mdtC b2076 0.26 -5.9 2,157,272 - mdtC b2076 0.28 +1.1 2,157,330 + mdtC b2076 0.30 +0.4 2,157,378 + mdtC b2076 0.31 -2.7
Or see this region's nucleotide sequence